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Research Articles

Effects of starvation on the expression of feeding related neuropeptides in the larval zebrafish hypothalamus

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  • Institute of Brain Science, Fudan University, Shanghai 200032, China

Received date: 2016-03-14

  Online published: 2016-09-20

Supported by

[Supported by the National Nature Science Foundation of China(No; 31171074)]

Abstract

Vertebrate feeding behavior is regulated by neuropeptide Y (NPY), GALANIN and GMAP prepropeptide (GAL), agouti related neuropeptide (AGRP) and proopiomelanocortin (POMC) in the hypothalamus. However, there are few studies on the relationship between these neuropeptides and feeding in zebrafish larvae. In the present study, real-time quantitative PCR and in situ hybridization were applied to examine the expression levels of npy, galanin, agrp and pomca in the hypothalamus of zebrafish larvae after starvation and re-feeding. The results showed the expression of agrp and galanin increased significantly after starvation compared to the control group, whilst the expression of pomca decreased significantly compared to control. If the animals were re-fed for two days after starvation, the expression of pomca, agrp and galanin showed no significant difference from the control. Expression of npy did not alter in either condition. These results indicate that starvation increases expression levels of agrp and galanin, and reduces the pomca expression. In addition, these starvation-induced changes can be reversed by re-feeding.

Key words: zebrafish; npy; galanin; agrp; pomca

Cite this article

Shanshan Liu, Cuizhen Zhang, Gang Peng . Effects of starvation on the expression of feeding related neuropeptides in the larval zebrafish hypothalamus[J]. Hereditas(Beijing), 2016 , 38(9) : 821 -830 . DOI: 10.16288/j.yczz.16-087

References

[1] Bolborea M, Dale N. Hypothalamic tanycytes: potential roles in the control of feeding and energy balance. Trends Neurosci , 2013, 36(2): 91-100.
[2] Larhammar D. Evolution of neuropeptide Y, peptide YY and pancreatic polypeptide. Regul Pept , 1996, 62(1): 1- 11.
[3] Tatemoto K, Carlquist M, Mutt V. Neuropeptide Y-a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature , 1982, 296(5858): 659-660.
[4] Dumont Y, Martel JC, Fournier A, St-Pierre S, Quirion R. Neuropeptide Y and neuropeptide Y receptor subtypes in brain and peripheral tissues. Prog Neurobiol , 1992, 38(2): 125-167.
[5] Williams G, Harrold JA, Cutler DJ. The hypothalamus and the regulation of energy homeostasis: lifting the lid on a black box. Proc Nutr Soc , 2000, 59(3): 385-396.
[6] Yoshitake T, Wang FH, Kuteeva E, Holmberg K, Yamaguchi M, Crawley JN, Steiner R, Bartfai T, Ögren SO, Hökfelt T, Kehr J. Enhanced hippocampal noradrenaline and serotonin release in galanin-overexpressing mice after repeated forced swimming test. Proc Natl Acad Sci USA , 2004, 101(1): 354-359.
[7] Millón C, Flores-Burgess A, Narváez M, Borroto-Escuela DO, Santín L, Parrado C, Narváez JA, Fuxe K, Díaz- Cabiale Z. A role for galanin N-terminal fragment (1-15) in anxiety- and depression-related behaviors in rats. Int J Neuropsychopharmacol , 2015, 18(3), doi:10.1093/ijnp/ pyu064.
[8] Misawa K, Misawa Y, Kanazawa T, Mochizuki D, Imai A, Endo S, Carey TE, Mineta H. Epigenetic inactivation of galanin and GALR1/2 is associated with early recurrence in head and neck cancer. Clin Exp Metastasis , 2016, 33(2): 187-195.
[9] Zafar MI, Hu CN, Liu DF, Shafqat RA, Gao F. Insulin detemir causes lesser weight gain in comparison to insulin glargine: role on hypothalamic NPY and galanin. J Diabetes Res , 2014, 2014: 458104.
[10] Shukla C, Basheer R. Metabolic signals in sleep regulation: recent insights. Nat Sci Sleep , 2016, 8(1): 9-20.
[11] Huang H, Lee SH, Ye CP, Lima IS, Oh BC, Lowell BB, Zabolotny JM, Kim YB. ROCK1 in AgRP neurons regulates energy expenditure and locomotor activity in male mice. Endocrinology , 2013, 154(10): 3660-3670.
[12] Wu YH, Patchev AV, Daniel G, Almeida OFX, Spengler D. Early-life stress reduces DNA methylation of the Pomc gene in male mice. Endocrinology , 2014, 155(5): 1751- 1762.
[13] Anderson EJ, Çakir I, Carrington S, Cone R, Ghamari- Langroudi M, Gillyard T, Gimenez LE, Litt MJ. 60 years of POMC: Regulation of feeding and energy homeostasis by α-MSH. J Mol Endocrinol , 2016, 56(4): T157- T174.
[14] Li L, Luo LF. Zebrafish as the model system to study organogenesis and regeneration. Hereditas ( Beijing ), 2013, 35(4): 421-432. 李礼, 罗凌飞. 以斑马鱼为模式动物研究器官的发育与再生. 遗传, 2013, 35(4): 421-432.
[15] Parker MO, Brock AJ, Walton RT, Brennan CH. The role of zebrafish ( Danio rerio ) in dissecting the genetics and neural circuits of executive function. Front Neural Circuits , 2013, 7: 63.
[16] Morin C, de Souza MA, Müller CP, Hardigan P, Spieler RE. Active avoidance learning in zebrafish ( Danio rerio )- the role of sensory modality and inter-stimulus interval. Behav Brain Res , 2013, 248: 141-143.
[17] Li HH, Huang P, Dong W, Zhu ZY, Liu D. A brief history of zebrafish research-toward biomedicine. Hereditas ( Beijing ), 2013, 35(4): 310-320. 李辉辉, 黄萍, 董巍, 朱作言, 刘东. 斑马鱼研究走向生物医学. 遗传, 2013, 35(4): 310-320.
[18] Kalueff AV, Gebhardt M, Stewart AM, Cachat JM, Brimmer M, Chawla JS, Craddock C, Kyzar EJ, Roth A, Landsman S, Gaikwad S, Robinson K, Baatrup E, Tierney K, Shamchuk A, Norton W, Miller N, Nicolson T, Braubach O, Gilman CP, Pittman J, Rosemberg DB, Gerlai R, Echevarria D, Lamb E, Neuhauss SCF, Weng W, Bally-Cuif L, Schneider H. Towards a comprehensive catalog of zebrafish behavior 1.0 and beyond. Zebra
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