We obtained a glabrous leaf and hull mutant from a population of radiation mutagenesis of an indica rice cultivar R401. The mutant produced smooth leaves and hairless glumes under normal growth conditions. An F2 population was developed from a cross between a japonica cultivar Nipponbare and the glabrous leaf and hull mutant. By investigating the performance of the F2 population, we found that the mutant phenotype was controlled by a single recessive gene, temporarily designated GLR3. Bulked segregant analysis (BSA) based on the F2 mapping population revealed that GLR3 is located on chromosome 6. By analyzing 417 typical glabrous leaf F2 plants using molecular markers, GLR3 was mapped to a 0.2 cM interval between InDel markers ID27101 and ID27199, and the physical distance between the two markers is 98 kb. Thus we have mapped the gene GLR3, and our work will provide basis for future mechanistic analysis of GLR3 function.
Haibing Song, Bin Wang, Renjie Chen, Xiaoya Zheng, Shibo Yu, Tao Lan
. Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)[J]. Hereditas(Beijing), 2016
, 38(11)
: 1012
-1019
.
DOI: 10.16288/j.yczz.16-114
[1] Zhao CZ, Yang CD, Wu LB, Qi XF, Huang FS, Hu PS, Luo J. Studies of culture character in glabrous rice ( Oryza sativa L.). Acta Agron Sin , 1999, 25(1): 82-85. 赵成章, 杨长登, 吴连斌, 戚秀芳, 黄发松, 胡培松, 罗矩. 光身稻的培养特性研究. 作物学报, 1999, 25(1): 82-85.
[2] Johnson HB. Plant pubescence: An ecological perspective. Bot Rev , 1975, 41(3): 233-258.
[3] Szymanski DB, Lloyd AM, Marks MD. Progress in the molecular genetic analysis of trichome initiation and morphogenesis in Arabidopsis . Trends Plant Sci , 2000, 5(5): 214-219.
[4] Pu L, Suo JF, Xue YB. Molecular control of plant trichome development. Acta Genet Sin , 2003, 30(11): 1078-1084. 普莉, 索金凤, 薛勇彪. 植物表皮毛发育的分子遗传控制. 遗传学报, 2003, 30(11): 1078-1084.
[5] Serna L, Martin C. Trichomes: different regulatory networks lead to convergent structures. Trends Plant Sci , 2006, 11(6): 274-280.
[6] Ishida T, Kurata T, Okada K, Wada T. A genetic regulatory network in the development of trichomes and root hairs. Annu Rev Plant Biol , 2008, 59: 365-386.
[7] Yan A, Pan JB, An LZ, Gan YB, Feng HY. The responses of trichome mutants to enhanced ultraviolet-B radiation in Arabidopsis thaliana . J Photochem Photobiol B , 2012, 113: 29-35.
[8] Herman PL, Marks MD. Trichome development in Arabidopsis thaliana . II. Isolation and complementation of the GLABROUS1 gene. Plant Cell , 1989, 1(11): 1051-1055.
[9] Rerie WG, Feldmann KA, Marks MD. The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis . Genes Dev , 1994, 8(12): 1388-1399.
[10] Zhao HT, Wang XX, Zhu DD, Cui SJ, Li X, Cao Y, Ma LG. A single amino acid substitution in IIIf subfamily of basic Helix-Loop-Helix transcription factor AtMYC1 leads to trichome and root hair patterning defects by abolishing its interaction with partner proteins in Arabidopsis . J Biol Chem , 2012, 287(17): 14109-14121.
[11] Walker AR, Davison PA, Bolognesi-Winfield AC, James CM, Srinivasan N, Blundell TL, Esch JJ, Marks MD, Gray JC. The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis , encodes a WD40 repeat protein. Plant Cell , 1999, 11(7): 1337-1350.
[12] Di Cristina M, Sessa G, Dolan L, Linstead P, Baima S, Ruberti I, Morelli G. The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development. Plant J , 2002, 10(3): 393-402.
[13] Johnson CS, Kolevski B, Smyth DR. TRANSPARENT TESTA GLABRA2 , a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor. Plant Cell , 2002, 14(6): 1359-1375.
[14] Hülskamp M, Miséra S, Jürgens G. Genetic dissection of trichome cell development in Arabidopsis . Cell , 1994, 76(3): 555-566.
[15] Yu N, Cai WJ, Wang SC, Shan CM, Wang LJ, Chen XY. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana . Plant Cell , 2010, 22(7): 2322-2335.
[16] Gan LJ, Xia K, Chen JG, Wang SC. Functional characterization of TRICHOMELESS2, a new single-repeat R3 MYB transcription factor in the regulation of trichome patterning in Arabidopsis . BMC Plant Biol , 2011, 11: 176.
[17] Matías-Hernández L, Aguilar-Jaramillo AE, Osnato M, Weinstain R, Shani E, Suárez-López P, Pelaz S. TEMPRANILLO reveals the mesophyll as crucial for epidermal trichome formation. Plant Physiol , 2016, 170(3): 1624-1639.
[18] Gao Y, Gong XM, Cao WH, Zhao JF, Fu LQ, Wang XC, Schumaker KS, Guo Y. SAD2 in Arabidopsis functions in trichome initiation through mediating GL3 function and regulating GL1 , TTG1 and GL2 expression. J Integr Plant Biol , 2008, 50(7): 906-917.
[19] Reiner T, Hoefle C, Huesmann C, Ménesi D, Fehér A, Hückelhoven R. The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching. Plant Cell Rep , 2015, 34(3): 457-468.
[20] Yu DS, Yu
