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Hereditas(Beijing) >

2017 , Vol. 39 >Issue 2: 156 - 165

DOI: https://doi.org/10.16288/j.yczz.16-327

Original article

Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis

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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2016-09-22

  Revised date: 2016-12-04

  Online published: 2017-02-10

Supported by

Key Scientific and Technological Innovation Team Project of Zhejiang Province Department of Science and Technology(2012R10029-07, 2010R50029);Foundation of Ningbo Science and Technology(2013C10018);the Outstanding (Postgraduate) Dissertation Growth Foundation of Ningbo University(py2014020)

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Abstract

β-carotenoid hydroxylase (CHYB) is an important rate-limiting enzyme in the biosynthesis of plant carotenoid. In this study, chyb1 and chyb2, two gene families in Dunaliella viridis were obtained by RNA-seq. The fragment of promoters of CHYB family genes, 1 080 bp for chyb1 (GenBank No. KY012338) and 1 155 bp for chyb2 (GenBank No. KY012339) were cloned by the Genome Walking Technology, respectively. Cis-acting elements of two promoters were analyzed by Plantcare soft. The results show that the chyb1 gene promoter contains more cis-acting elements in responses to abiotic stresses, such as methyl jasmonate, arachidonic acid, acetylsalicylic acid, and so on. On the other hand, the chyb2 promoter contains more cis-acting elements in response to light stress. qRT-PCR results show that the mRNA expression levels of CHYBs are modulated by their promoters, and different CHYB gene families response to distinct stresses.

Key words： Dunaliella viridis; β-carotenoid hydroxylase; gene family; promoter; stress

Cite this article

Shuaiqi Zhu,Yifu Gong,Li Zhang,Kai Yu,Heyu Wang,Xiaojun Yan . Expression analysis of different β-carotenoid hydroxylase gene families in stress response in Dunaliella viridis[J]. Hereditas(Beijing), 2017 , 39(2) : 156 -165 . DOI: 10.16288/j.yczz.16-327

References

[1] Liu HY, Xin NH. Research progress on β-carotene. J Sault Chem Ind, 2013, 42(1): 18-21.
[1] 刘洪岩, 辛乃宏. β-胡萝卜素的研究进展. 盐业与化工, 2013, 42(1): 18-21.
[2] Lin FM, Chen LR, Lin EH, Ke FC, Chen HY, Tsai MJ, Hsiao PW. Compounds from Wedelia chinensis synergistically suppress androgen activity and growth in prostate cancer cells. Carcinogenesis, 2007, 28(12): 2521-2529.
[3] Mathews-Roth MM. Beta carotene therapy for erythropoietic protoporphyria and other photosensitivity diseases. Arch Dermatol, 1977, 113(9): 1229-1232.
[4] Cui GH, Huang LQ, Tang XJ, Qiu D, Wang XY, Fu GF. Functional genomics studies of Salvia miltiorrhiza I establish cDNA microarray of S. miltiorrhiza. China J Chin Mater Med, 2007, 32(12): 1137-1141.
[4] 崔光红, 黄璐琦, 唐晓晶, 邱德, 王学勇, 付桂芳. 丹参功能基因组学研究I-cDNA芯片的构建. 中国中药杂志, 2007, 32(12): 1137-1141.
[5] Kato M, Ikoma Y, Matsumoto H, Sugiura M, Hyodo H, Yano M. Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit. Plant Physiol, 2004, 134(2): 824-837.
[6] Zhang L, Gong YF, Liu XD, Zhang WQ. Cloning and expression analysis of β-carotene hydroxylase gene (chyb) from Dunaliella salina. J Agric Biotechnol, 2013, 21(8): 920-930.
[6] 章丽, 龚一富, 刘晓丹, 张文青. 盐生杜氏藻β-胡萝卜素羟化酶基因(chyb)的克隆及表达分析. 农业生物技术学报, 2013, 21(8): 920-930.
[7] Linden H. Carotenoid hydroxylase from Haematococcus pluvialis: cDNA sequence, regulation and functional complementation. Biochim Biophys Acta, 1999, 1446(3): 203-212.
[8] Li YT, Huang JC, Sandmann G, Chen F. Glucose sensing and the mitochondrial alternative pathway are involved in the regulation of astaxanthin biosynthesis in the dark- grown Chlorella zofingiensis (Chlorophyceae). Planta, 2008, 228(5): 735-743.
[9] Li QL, Farre G, Naqvi S, Breitenbach J, Sanahuja G, Bai C, Sandmann G, Capell T, Christou P, Zhu CF. Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation. Transgenic Res, 2010, 19(6): 1053-1068.
[10] Bhosale P, Teredesai PV, Jin LH, Ermakov IV, Gellermann W, Bernstein PS. Production of deuterated zeaxanthin by Flavobacterium Multivorum and its detection by resonance Raman and mass spectrometric methods. Biotechnol Lett, 2005, 27(21): 1719-1723.
[11] Du H, Wang NL, Cui F, Li XH, Xiao JH, Xiong LZ. Characterization of the β-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Plant Physiol, 2010, 154(3): 1304-1318.
[12] Tian L, Musetti V, Ki
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