Protein post-translational modification by ubiquitination is essential for the activity and stability of proteins in the eukaryotic life cycle. In the past few years, it has been found that ubiquitination subtly modulates the abscisic acid (ABA) signaling pathway to regulate plant growth, development and stress responses, such as drought, salinity and cold stress responses. In this review, how the ubiquitin-proteasome system and ubiquitination-related membrane trafficking pathway affect ABA synthesis and signal transduction will be addressed and analysed. Also, the challenging questions in this field will be raised. These comprehensive views on the regulatory role of ubiquitination modification in the ABA pathway will shed light on future researches on how the ubiquitination-related process affects other hormone signaling pathways.