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Hereditas(Beijing) >

2017 , Vol. 39 >Issue 9: 828 - 836

DOI: https://doi.org/10.16288/j.yczz.16-403

miR-101a targeting EZH2 promotes the differentiation of goat skeletal muscle satellite cells

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  • 1 Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu 611130, China
    2 Institute of Animal Science, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850009, China

Received date: 2017-02-22

  Revised date: 2017-04-25

  Online published: 2017-10-21

Supported by

the Science and Technology Program of Sichuan Province(2016NYZ0045);Tibet Autonomous Region Science and Technology Major Project(2015, Z2016B01N04-06)

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Abstract

miR-101a promotes the differentiation of goat skeletal muscle satellite cells (SMSCs), as we previously reported, but the underpinning mechanism remains to be illuminated. In this study, we predicted the target gene of miR-101a by employing online softwares PicTar, TargetScan and miRanda, and found that enhancer of zeste homologue 2 (EZH2) was targeted by miR-101a. Further we identified that EZH2 contained miR-101a binding sites at its 3'UTR by using the dual-luciferase reporter assay system. In addition, we showed that during SMSC differentiation, the downregulated levels of EZH2 mRNA and protein were accompanied by increasing miR-101a expression via qRT-PCR and Western blot. Additionally, the expression of EZH2 significantly increased (P<0.01) when miR-101a was suppressed, whereas overexpressing miR-101a almost had no effect on EZH2 expression (P>0.05). These data demonstrated that miR-101a promotes SMSC differentiation directly through EZH2, which provides a theoretical reference for further elucidating the mechanism of miR-101a in SMSC differentiation.

Key words： miR-101a; goat; skeletal muscle satellite cells (SMSCs); enhancer of zeste homologue 2(EZH2)

Cite this article

Juntao Li,Wei Zhao,Dandan Li,Jing Feng,Gui Ba,Tianzeng Song,Hongping Zhang . miR-101a targeting EZH2 promotes the differentiation of goat skeletal muscle satellite cells[J]. Hereditas(Beijing), 2017 , 39(9) : 828 -836 . DOI: 10.16288/j.yczz.16-403

References

[1] Fujiwara T, Yada T. miRNA-target prediction based on transcriptional regulation. BMC Genomics, 2013, 14(S2): S3.
[2] Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight. Nat Rev Genet, 2008, 9(2): 102-114.
[3] Zhao Y, Srivastava D. A developmental view of microRNA function. Trends Biochem Sci, 2007, 32(4): 189-197.
[4] Chen JF, Tao YZ, Li J, Deng ZL, Yan Z, Xiao X, Wang DZ. MicroRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. J Cell Biol, 2010, 190(5): 867-879.
[5] Wright WE, Sassoon DA, Lin VK. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell, 1989, 56(4): 607-617.
[6] Anderson C, Catoe H, Werner R. MIR-206 regulates connexin43 expression during skeletal muscle development. Nucleic Acids Res, 2006, 34(20): 5863-5871.
[7] Goljanek-Whysall K, Sweetman D, Münsterberg AE. microRNAs in skeletal muscle differentiation and disease. Clin Sci, 2012, 123(11): 611-625.
[8] Crist CG, Montarras D, Pallafacchina G, Rocancourt D, Cumano A, Conway SJ, Buckingham M. Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. Proc Natl Acad Sci USA, 2009, 106(32): 13383-13397.
[9] Kuehbacher A, Urbich C, Zeiher AM, Dimmeler S. Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis. Circ Res, 2007, 101(1): 59-68.
[10] Obernosterer G, Leuschner PJF, Alenius M, Martinez J. Post-transcriptional regulation of microRNA expression. RNA, 2006, 12(7): 1161-1167.
[11] Hao YB, Gu XB, Zhao Y, Greene S, Sha W, Smoot DT, Cailfano J, Wu TC, Pang XW. Enforced expression of miR-101 inhibits prostate cancer cell growth by modulating the COX-2 pathway in vivo. Cancer Prev Res, 2011, 4(7): 1073-1083.
[12] Buechner J, T?mte E, Haug BH, Henriksen JR, L?kke C, Fl?gstad T, Einvik C. Tumour-suppressor microRNAs let-7 and mir-101 target the proto-oncogene MYCN and inhibit cell proliferation in MYCN-amplified neuroblastoma. Br J Cancer, 2011, 105(2): 296-303.
[13] Wang HJ, Ruan HJ, He XJ, Ma YY, Jiang XT, Xia YJ, Ye ZY, Tao HQ. MicroRNA-101 is down-regulated in gastric cancer and involved in cell migration and invasion. Eur J Cancer, 2010, 46(12): 2295-2303.
[14] Pan ZW, Sun XL, Shan HL, Wang N, Wang JH, Ren JS, Feng SY, Xie LJ, Lu CY, Yuan Y, Zhang Y, Wang Y, Lu YJ, Yang BF. MicroRNA-101 inhibited postinfarct cardiac fibrosis and improved left ventricular compliance via the FBJ osteosarc
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