The “new favorite” of gene editing technology—single base editors
Received date: 2017-11-27
Revised date: 2017-12-08
Online published: 2017-12-20
Supported by
the Natural Science Foundation of China(81670470)
Programmable nucleases are cutting edge genetic technology which edits targeted DNA sequences through generation of site-specific double-strand DNA breaks (DSBs). To improve the efficiency and precision of genetic modification, scientists have developed a single-base editing system (base editor) through combining of CRISPR/Cas9 system with cytosine deaminase. Compared with Cas9 system, this base editor can convert cytosine to thymine (C > T) at specific site more efficiently without inducing DSBs to avoid generation of indels. However, the base editor can only generate transition of pyrimidine but could not modify purines. Recently, Nature published a novel base editing system to convert adenine to guanine (ABEs, adenine base editors) through fusion of Cas9 nickase to a modified deaminase which is evolved through screening of random library based on tRNA adenine deaminase from E. coli. Here, we summarize the development of single-base editing tools and the latest research progress, especially the optimization process of ABEs, as well as the potential directions of the base editors.
Yu Wei,Xiaohui Zhang,Dali Li . The “new favorite” of gene editing technology—single base editors[J]. Hereditas(Beijing), 2017 , 39(12) : 1115 -1121 . DOI: 10.16288/j.yczz.17-389
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