CRISPR/Cas systems in genome engineering of bacteriophages
Received date: 2017-12-25
Revised date: 2018-02-06
Online published: 2018-04-04
Supported by
Supported by the National Science and Technology Major Project of China(2014ZX0801105B002);and the National High Technology Research & Development Project(2006AA09Z420)
Researches on CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated genes) systems, that are adaptive immunity systems encoded by prokaryotes, have promoted the development of new genome-editing tools. Bacteriophages are not only the driving elements for the evolution of prokaryotes’ CRISPR arrays, but also the targets of the CRISPR/Cas systems. Studies on functional genomics of bacteriophages have been lagging behind the discovery of new phage strains and the sequencing of their genomes. CRISPR/Cas systems-driven genome engineering of bacteriophages provides a novel approach for bacteriophage functional genomics. This review comments on a few profound cases of genome engineering of bacteriophages that employed the CRISPR/Cas systems, and compares multiple procedures illustrating common or distinct features as well as advantages and disadvantages underlying each procedure. We design new applications of the CRISPR/Cas systems coupled with bacteriophage recombination systems, discuss their potential constraints, and offer suggestions for each option.
Caijiao Liang, Fanmei Meng, Yuncan Ai . CRISPR/Cas systems in genome engineering of bacteriophages[J]. Hereditas(Beijing), 2018 , 40(5) : 378 -389 . DOI: 10.16288/j.yczz.17-419
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