Analyses on distribution and structure of <i>bla</i><sub>CARB-2</sub> in Klebsiella pneumoniae

Licheng Zhu,Junwan Lu,Jian Wang,Teng Xu,Juanhua Xu

doi:10.16288/j.yczz.18-034

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Hereditas(Beijing) >

2018 , Vol. 40 >Issue 7: 593 - 600

DOI: https://doi.org/10.16288/j.yczz.18-034

Research Articles

Analyses on distribution and structure of bla_CARB-2 in Klebsiella pneumoniae

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^1. College of Pharmaceutical Sciences,Zhejiang University, Hangzhou 310000, China
^2. College of Medicine and Health, Lishui University, Lishui 323000, China
^3. School of Laboratory and Life Sciences, Wenzhou Medical University, Wenzhou 325000, China
^4. Department of Laboratory Sciences, Ningbo First Hospital, Ningbo 315000, China

Received date: 2018-03-27

Revised date: 2018-05-31

Online published: 2018-05-31

Supported by

Supported by National Natural Science Foundation of China(80215049)

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Abstract

In order to characterize the structure of the beta-lactamase gene and its corresponding mobile genetic elements in Klebsiella pneumoniae, the beta-lactamase genes from 240 clinical Klebsiella pneumoniae isolates were studied. bla_CARB-2, a newly characterized gene, was extensively investigated utilizing next-generation sequencing, PCR, molecular cloning, conjugation, and comparative genomics analysis. We identified 11 beta-lactamase genes among the 240 clinical Klebsiella pneumoniae isolates; the bla_CARB-2 gene exists only in one specific isolate (Klebsiella pneumoniae KP1276) (1/240, 0.42%). The bla_CARB-2 gene lies on a conjugative plasmid pKP1276-82, a 182,450-bp plasmid, which encodes 222 open reading frames. The plasmid has seven resistance genes, termed bla_CARB-2, bla_KLUC, aadA1, aadA2, cmlA1, dfrA1, and sul2. Among these genes, bla_CARB-2 was identified for the first time in Klebsiella pneumoniae. Four of these resistance genes and an int gene form a class 1 integron (int-bla_CARB-2-aadA2-cmlA1-aadA1). Further studies show that the bla_CARB-2, aadA2, and cmlA1 genes are resistant to their corresponding antibiotics and the bla_CARB-2 exhibits higher resistance activities to penicillin beta-lactams. These results reveal the possibility of horizontal transfer of the resistance genes and dissemination of resistance among bacteria of different genera or species of Enterobacteriaceae.

Key words： Klebsiella pneumoniae; bla_CARB-2; class 1 integron; cloning; antibiotics resistance

Cite this article

Licheng Zhu,Junwan Lu,Jian Wang,Teng Xu,Juanhua Xu . Analyses on distribution and structure of bla_CARB-2 in Klebsiella pneumoniae[J]. Hereditas(Beijing), 2018 , 40(7) : 593 -600 . DOI: 10.16288/j.yczz.18-034

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