Progress and application of genome-edited pigs in biomedical research
Received date: 2018-01-06
Revised date: 2018-05-18
Online published: 2018-07-05
Supported by
Supported by the National Natural Science Foundation of China(1772555)
Genome editing technologies (GETs) can precisely alter the genomic sequences and modify the genetic information at the target site of an organism. Since the beginning of the 21st century, the GETs, including zinc finger nucleases (ZFN), transcription-activating-like receptor factor (TALEN), and clustered regularly interspaced short palindromic repeats/Cas endonucleases (CRISPR/Cas), have been successively developed. The GETs can easily engineer the targeted genomic site of animals to exhibit a desired phenotype(s), thereby providing valuable tools in biomedical research. The pigs are closely related to human, in terms of similarities in physiological properties and pathogenic characters. Thus, pigs have been used as important animal models in studies of human disease, xenotransplantation, and humanized organs regeneration. In this review, we summarize the development of the three GETs, research progress of genome-edited pigs as disease models and organ donors for xenotransplantation, and the prospects of their applications in future biomedical research.
Yaoqiang Huang,Guoling Li,Huaqiang Yang,Zhenfang Wu . Progress and application of genome-edited pigs in biomedical research[J]. Hereditas(Beijing), 2018 , 40(8) : 632 -646 . DOI: 10.16288/j.yczz.18-026
| [1] | Hinnen A, Hicks JB, Fink GR . Transformation of yeast. Proc Natl Acad Sci USA, 1978,75(4):1929-1933. |
| [2] | Orr-Weaver TL, Szostak JW, Rothstein RJ . Yeast transformation: a model system for the study of recombination. Proc Natl Acad Sci USA, 1981,78(10):6354-6358. |
| [3] | Rothestein RJ . One step gene disruption in yeast. Methods Enzymol, 1983,101:202-211. |
| [4] | Thomas KR, Folger KR , Capecchi MR . High frequency targeting of genes to specific sites in the mammalian genome.Cell, 1986, 14; 44(3):419-428. |
| [5] | Rouet P, Smih F, Jasin ME . Expression of a site-specific endonuclease stimulates homologous recombination in mammalian cells. Proc Natl Acad Sci USA, 1994,91(13):6064-6068. |
| [6] | Smih F, Rouet P, Romanienko PJ, Jasin M . Double- strand breaks at the target locus stimulate gene targeting in embryonic stem cells. Nucleic Acids Res, 1995,23(24):5012-5019. |
| [7] | Kim YG, Cha J, Chandrasegaran S . Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.Proc Natl Acad Sci USA, 1996,93(3):1156-1160. |
| [8] | Christian M, Cermak T, Doyle EL, Schmidt C, Zhang F, Hummel A, Bogdanove AJ, Voytas DF . Targeting DNA double-strand breaks with TAL effector nucleases. Genetics, 2010,186(2):757-761. |
| [9] | Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A . Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol, 1987,169(12):5429-5433. |
| [10] | Mojica FJ, Díez-Villaseñor C, Soria E, Juez G . Biological significance of a family of regularly spaced repeats in the genomes of Archaea, Bacteria and mitochondria. Mol Microbiol, 2000,36(1):244-246. |
| [11] | Jansen R, Embden JD, Gaastra W, Schouls LM . Identification of genes that are associated with DNA repeats in prokaryotes. Mol Microbiol, 2002,43(6):1565-1575. |
| [12] | Vergunst AC, Hooykaas PJJ . Recombination in the plant genome and its application in biotechnology. Crit Rev Plant Sci, 1999,18(1):1-31. |
| [13] | Mengiste T, Paszkowski J . Prospects for the precise engineering of plant genomes by homologous recombination. Biol Chem, 1999,380(7-8):749-758. |
| [14] | Lieber MR . The mechanism of double-strand DNA break repair by the nonhomologous DNA end-joining pathway. Annu Rev Biochem, 2010,79:181-211. |
| [15] | Krejci L, Altmannova V, Spirek M, Zhao X . Homologous recombination and its regulation. Nucleic Acids Res, 2012,40(13):5795-5818. |
| [16] |