Screening and identification of <i>CKI1</i> upstream transcription regulators in <i>Arabidopsis</i>

Zhenning Liu, Li Yuan, Venkatesan Sundaresan, Xiaolin Yu

doi:10.16288/j.yczz.18-314

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Hereditas(Beijing) >

2019 , Vol. 41 >Issue 5: 430 - 438

DOI: https://doi.org/10.16288/j.yczz.18-314

Research Article

Screening and identification of CKI1 upstream transcription regulators in Arabidopsis

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^1. College of Agriculture and Forestry Sciences, Linyi University, Linyi 276000, China
^2. Department of Plant Biology, University of California, Davis CA 95616, USA
^3. Institute of Vegetable Sciences, Zhejiang University, Hangzhou 310058, China
^4. College of Horticulture, Northwest A & F University, Yangling 712100, China;

Received date: 2018-11-21

Revised date: 2019-02-28

Online published: 2019-04-02

Supported by

National Natural Science Foundation of China(31700272);National Natural Science Foundation of China(31460521);National Natural Science Foundation of China(31872110);the Natural Science Foundation of Shandong Province(ZR2017PC012);the Breeding Project of the Sci-tech Foundation of Zhejiang Province(2016C02051-6-1)

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Abstract

Arabidopsis CKI1 (cytokinin independent 1) is a histidine kinase protein involved in the two-component system, which can activate two-component signaling via the downstream histidine phospho-transfer proteins, playing the essential roles in central cell fate determination and development regulation in embryo sacs. However, studies on CKI1 upstream transcription regulators are still limited. In the present study, promoter activities with varying fragments were investigated, and CKI1 upstream transcription regulators were screened and identified by the yeast-one hybrid technique. Results indicated F5/R2 fragments located in the intron region showed promoter activities in embryo sacs, which is consistent with CKI1 full-length promoters. Then three tandem repeats of F5/R2 fragments were used to construct the bait expression vector, and Arabidopsis pistils were collected for cDNA library construction. Totally, 226 positive clones were screened by the yeast-one hybrid technique, 66 readable sequences were retrieved after removing sequences with low quality and redundant repeats, among which eight proteins could act as DNA-binding proteins. These results provided some important clues to study the molecular function of CKI1 in the transcription regulation network.

Key words： CKI1; transcription regulation; yeast-one hybrid; Arabidopsis thaliana

Cite this article

Zhenning Liu, Li Yuan, Venkatesan Sundaresan, Xiaolin Yu . Screening and identification of CKI1 upstream transcription regulators in Arabidopsis[J]. Hereditas(Beijing), 2019 , 41(5) : 430 -438 . DOI: 10.16288/j.yczz.18-314

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