Biogenesis, research methods, and functions of circular RNAs
Received date: 2019-04-09
Revised date: 2019-05-07
Online published: 2019-05-07
Supported by
Supported by the National Natural Science Foundation of China(31800566);Fujian Provincial Natural Science Foundation(2018J01608);The National Key R&D Program of China(2016YFD0600106);The National Key R&D Program of China(2018YFD0600101);The International Science and Technology Cooperation and Exchange Fund(KXGH17016);Fujian Provincial Science and Technology Innovation Team Project(118/KLA18069A))
The field of circular non-coding RNAs have been gradually attracted wide attention with the developments of high-throughput sequencing. In this review, we systematically summarize three driving models for circRNAs biogenesis: intron-pairing-driven, RNA binding protein-driven and lariat-driven. In addition, we also briefly introduce the current research methods of circRNAs, which include high-throughput library construction methods, identification through bioinformatics and common experimental verification. Here, we also systematically summarize the functions of circRNAs, including microRNA (miRNA) or protein sponges, regulating the alternative splicing (AS) and expression of host genes, and extensive translation. Finally, we provide a systematic characterization and the latest research progress of circRNAs, which provide a new perspective for further studies of circRNAs in plants.
Key words： circular RNAs; back-splicing; alternative splicing; sponge
Xuqing Liu,Yubang Gao,Liangzhen Zhao,Yuchen Cai,Huiyuan Wang,Miao Miao,Lianfeng Gu,Hangxiao Zhang . Biogenesis, research methods, and functions of circular RNAs[J]. Hereditas(Beijing), 2019 , 41(6) : 469 -485 . DOI: 10.16288/j.yczz.19-061
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