Design and exploration of genetic comprehensive experiments based on Ds insertion mutants
Received date: 2019-09-26
Revised date: 2019-11-01
Online published: 2019-11-06
Supported by
Supported by the National Natural Science Foundation of China Nos(30900884);Supported by the National Natural Science Foundation of China Nos(31671594);Teaching Reform and Research Projects of SCAU in 2017 No(JG17093)
The cultivation of innovative abilities has become an important guide for higher education in China. Strengthening the integrated knowledge to design experiments is an effective way to improve undergraduate students’ innovative abilities. Herein we designed a comprehensive experiment for molecular genetics by utilizing a rice Ds insertion mutant identified previously in our research project. In the comprehensive experiment, we adopt the method of scientific research as the main line of teaching and take the interesting phenotype of the rice mutant as the breakthrough point to reform and innovate genetics laboratory teaching. On the basis of this, we combined the progressive teaching method and guided the students to learn the TAIL-PCR skill and conduct an innovative experiment through expanding their knowledge. The comprehensive experiment will deepen students’ understandings of the relationship between genotypes and phenotypes, help them master the effective way of thinking and technologies for scientific research to further improve their ability of the integrated application capability of theory and practices.
Key words: comprehensive experiment; experimental teaching; gene mutation
Nan Li, Yajuan Li, Haibin Guo, Xiangqian Zhang . Design and exploration of genetic comprehensive experiments based on Ds insertion mutants[J]. Hereditas(Beijing), 2019 , 41(12) : 1148 -1155 . DOI: 10.16288/j.yczz.19-149
| [1] | Chen DF, Lu DR, Zhang FX, Zhang GF . The development of genetics teaching in China in the last four decades and its future prospect. Hereditas(Beijing), 2018,40(10):916-932. |
| [1] | 陈德富, 卢大儒, 张飞雄, 张根发 . 中国遗传学教学40年发展及展望. 遗传, 2018,40(10):916-932. |
| [2] | Li N, Guo HB, Li YJ, Lao MM . Design and practice of comprehensive experiment for cytogenetics. Res Explor Lab, 2019,38(9): 200-203,227. |
| [2] | 李楠, 郭海滨, 李亚娟, 劳媚媚 . 细胞遗传学综合性实验的设计与实践. 实验室研究与探索, 2019,38(09):200-203, 227. |
| [3] | Liu ZQ, Zhao YX, Fu XL, Li N . Design and exploration of genetic experiments for non-mendelian segregation. Hereditas(Beijing), 2019,41(3):262-270. |
| [3] | 刘自强, 赵苑秀, 傅雪琳, 李楠 . 偏孟德尔分离的遗传学实验设计与探讨. 遗传, 2019,41(3):262-270. |
| [4] | Qi P, Lin YS, Song XJ, Shen JB, Huang W, Shan JX, Zhu MZ, Jiang LW, Gao JP, Lin HX . The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1;3. Cell Res, 2012,22(12):1666-1680. |
| [5] | Segami S, Kono I, Ando T, Yano M, Kitano H, Miura K, Iwasaki Y . Small and round seed 5 gene encodes alpha-tubulin regulating seed cell elongation in rice. Rice, 2012,5(1):4. |
| [6] | Cho SH, Yoo SC, Zhang H, Pandeya D, Koh HJ, Hwang JY, Kim GT, Paek NC . The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol, 2013,198(4):1071-1084. |
| [7] | Jin J, Shi JL, Liu B, Liu YC, Huang Y, Yu Y, Dong AW . MORF-RELATED GENE702, a reader protein of trimethylated histone H3 lysine 4 and histone H3 lysine 36, Is involved in brassinosteroid-regulated growth and flowering time control in rice. Plant Physiol, 2015,168(4):1275-1285. |
| [8] | Liu Y, Whittier RF . Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics, 1995,25(3):674-681. |
| [9] | Liu F, Zhang XQ, Zhang ZM, Chen ZG, Zhu HT, Wang J, Zhang JL, Zhang GQ . Transpositional behaviour of the Ds element in the Ac/Ds system in rice. Chin Sci Bull, 2007,52(20):2789-2796. |
| [10] | Liu XD, Li YJ. General Genetics Comprehensive Experiment. Beijing: China Agriculture Press, 2011. |
| [10] | 刘向东, 李亚娟 . 普通遗传学综合性实验. 北京: 中国农业出版社, 2011. |
| [11] | Jeon JS, An G . Gene tagging in rice: a high throughput system for functional genomics. Plant Sci, 2001,161(2):211-219. |
| [12] | Park SH, Kim CM, Je BI, Park SH, Park SJ, Piao HL, Xuan YH, Choe MS, Satoh K, Kikuchi S, Lee KH, Cha YS, Ahn BO, Ji HS, Yun DW, Lee MC, Suh SC, Eun MY, Han CD . A Ds-insertion mutant of OSH6(Oryza sativa Homeobox 6) exhibits outgrowth of vestigial leaf-like structures, bracts, in rice. Planta, 2007,227(1):1-12. |
| [13] | Zhu QH, Dennis ES, Upadhyaya NM . Compact shoot and leafy head 1, a mutation affects leaf initiation and developmental transition in rice ( Oryza sativa L.). Plant Cell Rep, 2007,26(4):421-427. |
| [14] | Qiang GR, Wang H, Yang ZP, Wang HB, Fang WJ . Research and practice on teaching methods of college organic chemistry experiment. Res Explor Lab, 2017,36(03):200-202, 211. |
| [14] | 强根荣, 王红, 杨振平, 王海滨, 方文军 . 大学有机化学实验教学方法研究与实践. 实验室研究与探索, 2017,36(03):200-202, 211. |
| [15] | Zu Q, Liu JB, Sun YM, Hu K . Reform of experimental teaching method in colleges and universities. Exper Sci Technol, 2015,13(03):88-90. |
| [15] | 祖强, 刘加彬, 孙岳明, 胡凯 . 大学本科实验教学方法改革初析. 实验科学与技术, 2015,13(03):88-90. |
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