The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells
Received date: 2019-05-21
Revised date: 2019-11-19
Online published: 2019-12-05
Supported by
Supported by the National Natural Science Foundation of China No(31872322);Central University Innovation Research Fund No(2662017PY030)
Porcine skeletal muscle development is a complex biological process, and differentiation of skeletal muscle satellite cells is an important part of skeletal muscle development. In recent years, it has been found that lncRNA plays an important role in the differentiation of skeletal muscle satellite cells. Here we investigate the effect of lncRNA TCONS_00815878 on the differentiation of porcine skeletal muscle satellite cells. We first used qRT-PCR to detect the expression levels of TCONS_00815878 in six tissues (heart, spleen, lung, kidney, back muscles and leg muscles) of Yorkshire piglets within seven days of birth. At the same time, the expression levels of TCONS_00815878 at five different time points from the embryonic stage to the postnatal stage (35 d, 45 d, 55 d of embryos, and 7 d, 200 d of postpartum leg muscles) were examined. The expression of the differentiation marker genes MyoD, MyoG and MyHC was examined by knocking down TCONS_00815878 in porcine skeletal muscle satellite cells using antisense oligonucleotides (ASO). The target gene of TCONS_00815878 was predicted by bioinformatics analysis, and the function and pathway of its target gene were predicted online using DAVID software. The results showed that TCONS_00815878 had the highest expression level in pig myocardium and leg muscles. Within seven days after birth, TCONS_00815878 increased in the muscle tissue of pigs, and reached the peak of expression level on the 7th day. During the process of proliferation and differentiation of porcine skeletal muscle satellite cells, the expression level of TCONS_00815878 increased during the differentiation stage and peaked at 30 h of differentiation. After knocking down TCONS_00815878, the expression levels of MyoD, MyoG and MyHC were decreased, but the expression level of MyoD was significantly decreased (P<0.05). In addition, functional predictions revealed that the target gene of TCONS_00815878 is enriched in multiple biological processes, such as glycolysis and pyruvate metabolism, related to skeletal muscle satellite cell differentiation. This study speculates that lncRNA TCONS_00815878 may promote the differentiation of porcine skeletal muscle satellite cells.
Ziying Huang, Long Li, Qianqian Li, Xiangdong Liu, Changchun Li . The effect of lncRNA TCONS_00815878 on differentiation of porcine skeletal muscle satellite cells[J]. Hereditas(Beijing), 2019 , 41(12) : 1119 -1128 . DOI: 10.16288/j.yczz.19-146
| [1] | Groenen MAM, Archibald AL, Uenishi H, Tuggle CK, Takeuchi Y, Rothschild MF, Rogel-Gaillard C, Park C, Milan D, Megens HJ, Li ST, Larkin DM, Kim H, Frantz LAF, Caccamo M, Ahn H, Aken BL, Anselmo A, Anthon C, Auvil L, Badaoui B, Beattie CW, Bendixen C, Berman D, Blecha F, Blomberg J, Bolund L, Bosse M, Botti S, Bujie Z, Bystrom M, Capitanu B, Silva DC, Chardon P, Chen C, Cheng R, Choi SH, Chow W, Clark RC, Clee C, Crooijmans RPMA, Dawson HD, Dehais P, De Sapio F, Dibbits B, Drou N, Du ZQ, Eversole K, Fadista J, Fairley S, Faraut T, Faulkner GJ, Fowler KE, Fredholm M, Fritz E, Gilbert JGR, Giuffra E, Gorodkin J, Griffin DK, Harrow JL, Hayward A, Howe K, Hu ZL, Humphray SJ, Hunt T, Hornsh?j H, Jeon JT, Jern P, Jones M, Jurka J, Kanamori H, Kapetanovic R, Kim J, Kim JH, Kim KW, Kim TK, Larson G, Lee K, Lee KT, Leggett R, Lewin HA, Li YR, Liu WS, Loveland JE, Lu Y, Lunney JK, Ma J, Madsen O, Mann K, Matthews L, McLaren S, Morozumi T, Murtaugh MP, Narayan J, Nguyen DT, Ni PX, Oh SJ, Onteru S, Panitz F, Park EW, Park HS, Pascal G, Paudel Y, Perez-Enciso M, Ramirez-Gonzalez R, Reecy JM, Zas SR, Rohrer GA, Rund L, Sang YM, Schachtschneider K, Schraiber JG, Schwartz J, Scobie L, Scott C, Searle S, Servin B, Southey BR, Sperber G, Stadler P, Sweedler JV, Tafer H, Thomsen B, Wali R, Wang J, Wang J, White S, Xu X, Yerle M, Zhang GJ, Zhang JG, Zhang J, Zhao SH, Rogers J, Churcher C, Schook LB . Analyses of pig genomes provide insight into porcine demography and evolution. Nature, 2012,491(7424):393-398. |
| [2] | Almada AE, Wagers AJ . Molecular circuitry of stem cell fate in skeletal muscle regeneration, ageing and disease. Nat Rev Mol Cell Biol, 2016,17(5):267-279. |
| [3] | Sacco A, Doyonnas R, Kraft P, Vitorovic S, Blau HM . Self-renewal and expansion of single transplanted muscle stem cells. Nature, 2008,456(7221):502-506. |
| [4] | Allen RE, Merkel RA, Young RB . Cellular aspects of muscle growth: myogenic cell proliferation. J Anim Sci, 1979,49(1):115-127. |
| [5] | Buckingham M . Myogenic progenitor cells and skeletal myogenesis in vertebrates. Curr Opin Genet Dev, 2006,16(5):525-532. |
| [6] | Jathar S, Kumar V, Srivastava J, Tripathi V . Technological developments in lncRNA biology. Adv Exp Med Biol, 2017,1008:283-323. |
| [7] | Yang F, Yi F, Cao HQ, Liang ZC, Du Q . The emerging landscape of long non-coding RNAs. Hereditas(Beijing), 2014,36(5):456-468. |
| [7] | 杨峰, 易凡, 曹慧青, 梁子才, 杜权 . 长链非编码RNA研究进展. 遗传, 2014,36(5):456-468. |
| [8] | Lu C, Huang YH . Progress in long non-coding RNAs in animals. Hereditas(Beijing), 2017,39(11):1054-1065. |
| [8] | 路畅, 黄银花 . 动物长链非编码RNA研究进展. 遗传, 2017,39(11):1054-1065. |
| [9] | Weikard R, Demasius W, Kuehn C . Mining long noncoding RNA in livestock. Anim Genet, 2017,48(1):3-18. |
| [10] | Li H, Feng JC, Li GL, Wang X, Li MZ, Liu HF . The effect of lnc-RAP3 on 3T3-L1 preadipocyte differentiation in mouse. Hereditas(Beijing), 2018,40(9):758-766. |
| [10] | 李欢, 冯晋川, 李贵林, 王讯, 李明洲, 刘海峰 . Lnc- RAP3对小鼠3T3-L1前脂肪细胞分化的影响. 遗传, 2018,40(9):758-766. |
| [11] | Xie SH . Integrated analysis of miRNA and mRNA expression profiles of muscle development in Changbai and Lantang pigs[Dissertation]. Sun Yat-Sen University, 2017. |
| [11] | 谢水华 . 长白猪和蓝塘猪肌肉发育差异miRNA和mRNA表达谱的整合分析[学位论文]. 中山大学, 2017. |
| [12] | Tang ZL, Wu Y, Yang YL, Yang YCT, Wang ZS, Yuan JP, Yang Y, Hua CJ, Fan XH, Niu GL, Zhang YB, Lu ZJ, Li K . Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa. Sci Rep, 2017,7:43166. |
| [13] | Zhou ZY, Li AM, Adeola AC, Liu YH, Irwin DM, Xie HB, Zhang YP . Genome-Wide identification of long intergenic noncoding RNA genes and their potential association with domestication in pigs. Genome Biol Evol, 2014,6(6):1387-1392. |
| [14] | Yun L, Liu JP, Zhuang ZX, Yang LQ, Zhang RL, Ye XM, Cheng JQ . Real-time RT-PCR gene expression relative quantification REST ? software analysis compared with 2 (-ΔΔCT)method . J Trop Med, 2007,7(10):956-958. |
| [14] | 庾蕾, 刘建平, 庄志雄, 杨淋清, 张仁利, 叶小明, 程锦泉 . 实时RT-PCR基因表达相对定量REST ?软件分析与2 (-ΔΔCT)法比较 . 热带医学杂志, 2007,7(10):956-958. |
| [15] | Chen G, Shi TL, Shi LM . Characterizing and annotating the genome using RNA-seq data. Sci China Life Sci, 2017,60(2):116-125. |
| [16] | Djebali S, Davis CA, Merkel A, Dobin A, Lassmann T, Mortazavi A, Tanzer A, Lagarde J, Lin W, Schlesinger F, Xue C, Marinov GK, Khatun J, Williams BA, Zaleski C, Rozowsky J, R?der M, Kokocinski F, Abdelhamid RF, Alioto T, Antoshechkin I, Baer MT, Bar NS, Batut P, Bell K, Bell I, Chakrabortty S, Chen X, Chrast J, Curado J, Derrien T, Drenkow J, Dumais E, Dumais J, Duttagupta R, Falconnet E, Fastuca M, Fejes-Toth K, Ferreira P, Foissac S, Fullwood MJ, Gao H, Gonzalez D, Gordon A, Gunawardena H, Howald C, Jha S, Johnson R, Kapranov P, King B, Kingswood C, Luo OJ, Park E, Persaud K, Preall JB, Ribeca P, Risk B, Robyr D, Sammeth M, Schaffer L, See LH, Shahab A, Skancke J, Suzuki AM, Takahashi H, Tilgner H, Trout D, Walters N, Wang H, Wrobel J, Yu Y, Ruan X, Hayashizaki Y, Harrow J, Gerstein M, Hubbard T, Reymond A, Antonarakis SE, Hannon G, Giddings MC, Ruan Y, Wold B, Carninci P, Guigó R, Gingeras TR . Landscape of transcription in human cells. Nature, 2012,489(7414):101-108. |
| [17] | Deniz E, Erman B . Long noncoding RNA (lincRNA), a new paradigm in gene expression control. Funct Integr Genomics, 2017,17(2-3):135-143. |
| [18] | Jandura A, Krause HM . The new RNA world: growing evidence for long noncoding RNA functionality. Trends Genet, 2017,33(10):665-676. |
| [19] | Li YY, Chen XN, Sun H, Wang HT . Long non-coding RNAs in the regulation of skeletal myogenesis and muscle diseases. Cancer Lett, 2018,417:58-64. |
| [20] | Flynn RA, Chang HY . Long noncoding RNAs in cell-fate programming and reprogramming. Cell Stem Cell, 2014,14(6):752-761. |
| [21] | Qin CY, Cai H, Qing HR, Li L, Zhang HP . Recent advances on the role of long non-coding RNA H19 in regulating mammalian muscle growth and development. Hereditas(Beijing), 2017,39(12):1150-1157. |
| [21] | 秦辰雨, 蔡禾, 卿涵睿, 李利, 张红平 . 长链非编码RNA H19对哺乳动物肌肉生长发育的调控. 遗传, 2017,39(12):1150-1157. |
| [22] | Zhou R, Wang YX, Long KR, Jiang AA, Jin L . Regulatory mechanism for lncRNAs in skeletal muscle development and progress on its research in domestic animals. Hereditas(Beijing), 2018,40(4):292-304. |
| [22] | 周瑞, 王以鑫, 龙科任, 蒋岸岸, 金龙 . LncRNA调控骨骼肌发育的分子机制及其在家养动物中的研究进展. 遗传, 2018,40(4):292-304. |
| [23] | Li MX, Huang T, Ma LP, Liu Y, Li T, Gong HB, Qiu MY, Xie S, Sun XM . Cloning of porcine lncRNA-ENSSSCT00000018610 and its expression pattern in porcine ovarian follicles. Acta Vet Et Zootech Sin, 2018,49(9):1830-1839. |
| [23] | 李梦寻, 黄涛, 马力鹏, 刘乙, 李涛, 公红斌, 邱梅玉, 谢苏, 孙晓梅 . 猪lncRNA-ENSSSCT00000018610的克隆及其在猪卵泡中的表达. 畜牧兽医学报, 2018,49(9):1830-1839. |
| [24] | Picard B, Lefaucheur L, Berri C, Duclos MJ . Muscle fibre ontogenesis in farm animal species. Reprod Nutr Dev, 2002,42(5):415-431. |
| [25] | Lefaucheur L, Vigneron P . Post-natal changes in some histochemical and enzymatic characteristics of three pig muscles. Meat Sci, 1986,16(3):199-216. |
| [26] | Zammit PS, Heslop L, Hudon V, Rosenblatt JD, Tajbakhsh S, Buckingham ME, Beauchamp JR, Partridge TA . Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers. Exp Cell Res, 2002,281(1):39-49. |
| [27] | Liao Q, Liu CN, Yuan XY, Kang SL, Miao RY, Xiao H, Zhao GG, Luo HT, Bu DC, Zhao HT, Skogerb? G, Wu ZD, Zhao Y . Large-scale prediction of long non-coding RNA functions in a coding-non-coding gene co-expression network. Nucleic Acids Res, 2011,39(9):3864-3878. |
| [28] | Sanoudou D, Haslett JN, Kho AT, Guo SQ, Gazda HT, Greenberg SA, Lidov HGW, Kohane IS, Kunkel LM, Beggs AH . Expression profiling reveals altered satellite cell numbers and glycolytic enzyme transcription in nemaline myopathy muscle. Proc Natl Acad Sci USA, 2003,100(8):4666-4671. |
| [29] | Mangano KM, Sahni S, Kerstetter JE, Kenny AM, Hannan MT . Polyunsaturated fatty acids and their relation with bone and muscle health in adults. Curr Osteoporos Rep, 2013,11(3):203-212. |
| [30] | Lambadiari V, Triantafyllou K, Dimitriadis GD . Insulin action in muscle and adipose tissue in type 2 diabetes: The significance of blood flow. World J Diabetes, 2015,6(4):626-633. |
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号
