Genome editing in plants directed by CRISPR/Cas ribonucleoprotein complexes
Received date: 2020-01-13
Revised date: 2020-04-27
Online published: 2020-05-21
The CRISPR/Cas system is the most popular genome editing technology in recent years and has been widely used in crop improvement. Compared with introducing the CRISPR/Cas system into plant cells with DNA constructs, introducing CRISPR/Cas ribonucleoprotein (RNP) to perform genome editing excels in rapid action, low off-target rates and is free of DNA insertions in editing plants. However, efficient delivery of CRISPR/Cas RNP into plant cells and achieving high editing frequency are still very challenging, which limits the extensive implementation of CRISPR/Cas RNP-mediated genome editing in plants. In this review, we summarize the progress of protein and RNP delivery methods in plant cells, and provide new perspectives of further development and future applications of the CRISPR/Cas RNP technology in plant genome editing.
Key words： CRISPR/Cas; genome editing; ribonucleoprotein (RNP); DNA-free; plant transformation
Xia Li, Wan Shi, Lizhao Geng, Jianping Xu . Genome editing in plants directed by CRISPR/Cas ribonucleoprotein complexes[J]. Hereditas(Beijing), 2020 , 42(6) : 556 -564 . DOI: 10.16288/j.yczz.20-017
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