Related genes and characteristic analysis of trophoblast cells during early embryo developmental cessation
Received date: 2020-08-08
Revised date: 2020-10-02
Online published: 2020-10-15
Supported by
Supported by the National Nature Science Foundation of China No(31771662);the National Science and Technology Major Project No(2018YFC1002803-3)
Trophoblast cells play essential roles in the maintenance of normal embryo implantation, growth and development. The study of abnormal gene changes in trophoblastic cells from arrested embryos is helpful to understand the developmental mechanism of embryo developmental cessation or adverse pregnancy outcomes. In this study, we sequenced and analyzed the transcriptomes of the villi from ten women who have undergone abortion with either normal pregnancy or embryo development cessation. We found that there were 436 differentially expressed genes, of which 406 mRNA were significantly up-regulated and 32 mRNA were significantly down-regulated. Gene enrichment analysis showed that these genes were significantly enriched in immune-related functions and intercellular adhesion, such as lymphocyte activation, myeloid cell activation, extracellular matrix and collagen junction. And their potential regulatory pathways were enriched in terms of complement and coagulation cascade, extracellular matrix degradation. In addition, in this study the co-expression analysis of WGCNA was used to obtain the lncRNA with co-expression relationship with the differential genes. According to the different functions of the modules, two network diagrams were drawn, and four key genes were obtained, namely VSIG4, C1QC, CD36 and SPP1. These differential genes obtained in this study can be used as key molecules with potential effects on embryo development cessation. The enriched entries can provide a theoretical basis and new direction for further understanding of the etiology and mechanism of embryo development cessation or adverse pregnancy outcomes.
Key words： trophoblast; RNA-seq; enrichment analysis; genetic traits
Yue Zhang, Ying Feng, Fang Ma . Related genes and characteristic analysis of trophoblast cells during early embryo developmental cessation[J]. Hereditas(Beijing), 2020 , 42(10) : 1004 -1016 . DOI: 10.16288/j.yczz.20-144
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