Received date: 2020-11-23
Online published: 2021-01-27
Supported by
the National Science and Technology Major Project(2018ZX10101003-002-011);the Major Infectious Diseases Such as AIDS and Viral Hepatitis Prevention and Control Technology Major Projects(2018ZX10712-001-003)
Bacteria with the smallest genome contain genes necessary for self-sustaining replication only, giving the organisms advantages to serve as a potential industrial production platform. Many strains with reduced genomes have been constructed, owing to the development of high-throughput DNA sequencing and synthesis technology. This review first describes the concept of minimal genomes, summarizes the relevant research progress of bacterial essential genes, then systematically lists the work related to artificial reduction and synthesis of bacterial genomes, finally discusses the technical obstacles and limitations encountered in the process of designing and constructing reduced genomes, hoping to provide a theoretical basis for the experiment and application of artificially synthesized genomes.
Key words： bacteria; minimal genome; essential genes; synthetic biology; artificial design
Jinyu Li, Shan Yang, Yujun Cui, Tao Wang, Yue Teng
.
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