A method for constructing GLP-1 overexpression intestinal organoids
Received date: 2021-03-01
Revised date: 2021-04-14
Online published: 2021-07-20
Supported by
Supported by the National Key R&D Program of China No(2019YFA0110802)
As a potent insulinotrophic hormone, glucagon-like peptide 1 (GLP-1) is mainly secreted by intestinal L cells, which can effectively promote the release of insulin and thus reduce blood glucose. Therefore, GLP-1 and its analogs have a good prospect in the treatment of type 2 diabetes. In this study, we constructed mouse intestinal organoids that overexpress GLP-1 by optimizing the GLP-1 lentivirus infection method. We found that supernatants secreted by the GLP-1 overexpression organoids effectively enhanced glucose tolerance in wild-type and diabetic mouse. Thus, the GLP-1 overexpression organoids built in this study may provide a novel strategy for the treatment of type 2 diabetes.
Key words： GLP-1; type 2 diabetes; intestinal organoids
Zhiyang Zeng, Jiawei Lu, Xiya Cao, Xinyue Wang, Dali Li . A method for constructing GLP-1 overexpression intestinal organoids[J]. Hereditas(Beijing), 2021 , 43(7) : 694 -703 . DOI: 10.16288/j.yczz.20-423
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