Transcription factor OsMADS25 improves rice tolerance to cold stress
Received date: 2021-06-20
Revised date: 2021-08-21
Online published: 2021-10-12
Supported by
Supported by the Hunan Science and Technology Major Project No(2018NK1010);the Hunan Science and Technology Talents Support Project No(2019TJ-Q08);the Research Initiation Fund of Hunan Agricultural University No(20154/5407419002);the Open Research Fund of the State Key Laboratory of Hybrid Rice, Hybrid Rice Research Center No(2020KF05);the Hunan Province Natural Science Fund No(2019JJ50714)
Cold stress is the limiting factor of rice growth and production, and it is important to clone cold stress tolerant genes and cultivate cold tolerance rice varieties. The MADS transcription factors play an important role in abiotic stress signaling in rice. This study showed that OsMADS25 was up-regulated by low temperature and abscisic acid (ABA), suggesting that OsMADS25 may be involved in ABA-dependent signaling. The OsMADS25 overexpression vector, pCambia1300-221-OsMADS25-Flag, was constructed and introduced into the rice variety Zhonghua 11 (ZH11) through Agrobacterium tumefacian-mediated genetic transformation. Two homozygous lines with high expression levels were selected for phenotypic identification. OsMADS25 overexpression lines show significantly improved cold stress tolerance and the sensitivity to ABA at the seedling stage of rice. Reactive oxygen species (ROS) was detected by diaminobenzidine (DAB) staining and nitroblue tetrazolium (NBT) staining. After treatment with cold stress, little ROS accumulation was observed in OsMADS25 overexpression lines compared to wild-type ZH11. In conclusion, OsMADS25 plays a role in scavenging reactive oxygen species (ROS) and could improve rice tolerance to cold stress involved in ABA-dependent pathway.
Lingyue Yan, Haojian Zhang, Yuqing Zheng, Yunqi Cong, Citao Liu, Fan Fan, Cheng Zheng, Guilong Yuan, Gen Pan, Dingyang Yuan, Meijuan Duan . Transcription factor OsMADS25 improves rice tolerance to cold stress[J]. Hereditas(Beijing), 2021 , 43(11) : 1078 -1087 . DOI: 10.16288/j.yczz.21-217
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