The cutting edge of gene regulation approaches in model organism Drosophila
Received date: 2021-10-07
Revised date: 2021-12-21
Online published: 2022-01-04
Supported by
Supported by the National Natural Science Foundation of China Nos(20181300988);Supported by the National Natural Science Foundation of China Nos(20201300797);the National Key Technology Research and Development Program of the Ministry of Science and Technology of the People’s Republic of China No(2016YFE0113700)
Transgenic gene regulatory techniques play a critical role in biomedical fields since they are essential for researchers to study the molecular mechanisms of development and diseases. The currently prevalent transgenic techniques include specific gene mutation, transgenic RNAi and targeting activation, etc. Specifically, various gene regulatory tools have been developed to temporarily and spatially modulate genes in Drosophila melanogaster, which is a typical model organism with evolutionally conserved genome and without ethical issues in scientific research. Here, we introduce several gene regulatory techniques commonly used in Drosophila, including the principle, procedure, toolbox and related transgenic resource of CRISPR/Cas9-triggered heritable gene mutation system, next-generation transgenic RNAi and CRISPR/dCas9-based transcriptional activation (flySAM) approach. We wish to take this opportunity to raise the awareness of the importance of transgenic gene regulatory techniques development and related resource construction and thus to promote biomedical research progression.
Key words： Drosophila; gene regulation; CRISPR/Cas9; gene editing; transgenic RNAi; CRISPRa
Yuting Han, Bowen Xu, Yutong Li, Xinyi Lu, Xizhi Dong, Yuhao Qiu, Qinyun Che, Ruibao Zhu, Li Zheng, Xiaochen Li, Xu Si, Jianquan Ni . The cutting edge of gene regulation approaches in model organism Drosophila[J]. Hereditas(Beijing), 2022 , 44(1) : 3 -14 . DOI: 10.16288/j.yczz.21-347
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