Genome-wide identification and expression analysis of the PEBP genes in Arabidopsis pumila
Received date: 2021-08-24
Revised date: 2021-11-15
Online published: 2022-01-06
Supported by
Supported by the National Natural Science Foundation of China No(U1303302);the International Science and Technology Cooperation Project of Shihezi University No(GJHZ201806);the Talent Introduction Start-up Fund Project of Anhui Science and Technology University(NXYJ202001)
The members of the phosphatidylethanolamine-binding protein (PEBP) family harbor a conserved phosphatidylethanolamine-binding protein domain, of which the FT and TFL1 proteins constitute a plant florigen-antiflorigen system regulating flowering time and plant structure. Arabidopsis pumila is an early spring and ephemeral plant, which grows in the southern boundary of the Gulban Tonggut desert and has a good adaptability to the extreme environment. In this study, a genome-wide identification revealed that the A. pumila genome contains 11 PEBP genes (one MFT, two FT, two TSF, two TFL1, two CEN, and two BFT), all of which consist of four exons and three introns. Collinearity analysis showed that there are 11 pairs of collinearity relationships between A. pumila and A. thaliana, and A. lyrata, respectively. The PEBP gene family obviously has expanded in the A. pumila genome, with duplication of ApPEBP genes in the forms of whole genome duplication/segmental duplication. Tissue expression analysis showed that ApMFT was highly expressed in seeds; ApFT and ApBFT were mainly expressed in flowers and siliques; ApTFL1 was highly expressed in shoot apex; and ApCEN was highly expressed in roots. In addition, the expression profiles of ApPEBP genes under four abiotic stresses were also analyzed in this study. The results revealed that ApPEBP genes were generally up-regulated under drought stress (10% PEG6000) and were generally down-regulated under low temperature (4℃) stress. Under salt stress (250 mmol/L NaCl), ApFT1/2 were down-regulated, ApTFL1-1/2 were initially down-regulated and then up-regulated; and ApCEN1/2 were strikingly up-regulated. Under high temperature (40℃) stress, the expressions of ApFT1/2 and ApTFL1-1/2 were significantly down-regulated, but ApCEN1/2 were noticeably up-regulated. Collectively, ApCEN1/2 expressions were significantly up-regulated under salt, drought, and heat stresses. Protein interaction network analysis showed that A. pumila PEBP proteins interacted with proteins in flowering pathways and ribosomal proteins. These results suggested that ApPEBP genes play an important role(s) in the regulation of growth, development, and flowering transition of A. pumila in response to desert adversities.
Key words： ephemeral plant; Arabidopsis pumila; flowering transition; PEBP; adversity
Yongguang Li, Yuhuan Jin, Li Guo, Hao Ai, Ruining Li, Xianzhong Huang . Genome-wide identification and expression analysis of the PEBP genes in Arabidopsis pumila[J]. Hereditas(Beijing), 2022 , 44(1) : 80 -91 . DOI: 10.16288/j.yczz.21-305
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