Optimization of microsatellite genotyping system used for genetic diversity evaluation of <i>Ailuropoda melanoleuca</i>

Jie Kou, Yan Li, Peng Wang, Hong Liu, Jiawen Liu, Juan Wang, Ye Wang, Liang Zhang, Fujun Shen

doi:10.16288/j.yczz.21-348

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Hereditas(Beijing) >

2022 , Vol. 44 >Issue 3: 253 - 266

DOI: https://doi.org/10.16288/j.yczz.21-348

Technique and Method

Optimization of microsatellite genotyping system used for genetic diversity evaluation of Ailuropoda melanoleuca

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1. Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China
2. Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Chengdu 610081, China
3. School of Life Sciences and Engineering, Southwest University of Science and Technology, Mianyang 621010, China

Received date: 2021-10-08

Revised date: 2022-01-08

Online published: 2022-02-24

Supported by

Supported by the Chengdu Giant Panda Breeding Research Foundation No(CPF-2014-08);Chengdu Giant Panda Breeding Research Base No(CPF-2014-08);the Key R&D Project of Sichuan Province No(2018JZ0074)

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Abstract

Microsatellite DNA is one of the most widely used genetic markers of giant panda, especially in population size estimation, paternity testing, and genetic diversity analysis. However, there are few reports on the physical locations of microsatellite markers on the chromosomes of the giant panda (Ailuropoda melanoleuca) and research on the performance of microsatellite in genotyping system and the PCR amplification conditions. In this study, we analyzed the chromosomal locations and evaluated the application values of 34 microsatellite markers, based on the giant panda genome reference sequence (ASM200744v2). We optimized the PCR reaction systems and amplification procedures for these 34 microsatellite markers. We found the low value of the microsatellite marker of Ame-μ10 in genetic application, and the necessity in redesigning the primers for gpz-6. Our research helps to improve the reproducibility and reliability of genotyping results and is of great significance for promoting the establishment and standardized application of the “A Regulation for Giant Panda Population Genetic Archives” in giant panda conservation.

Key words： giant panda; genetic diversity; microsatellite; STR-PCR

Cite this article

Jie Kou, Yan Li, Peng Wang, Hong Liu, Jiawen Liu, Juan Wang, Ye Wang, Liang Zhang, Fujun Shen . Optimization of microsatellite genotyping system used for genetic diversity evaluation of Ailuropoda melanoleuca[J]. Hereditas(Beijing), 2022 , 44(3) : 253 -266 . DOI: 10.16288/j.yczz.21-348

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