Progress on Z genome biosynthetic pathway of bacteriophage
Received date: 2023-05-26
Revised date: 2023-08-17
Online published: 2023-08-18
Supported by
National Natural Science Foundation of China(32122024);Shanghai Frontiers Science Center for Bomacromolecules and Precision Medicine
There are abundant base modifications in bacteriophages’ genomes, mainly for avoiding the digestion of host endonucleases. More than 40 years ago, researchers discovered that 2-amino-adenine (Z) completely replaced adenine (A) and forms a complementary pairing with three hydrogen bonds with thymine (T) in the DNA of cyanophage S-2L, forming a distinct “Z-genome”. In recent years, researchers have discovered and validated the biosynthetic pathway of Z-genome in various bacteriophages, constituting a multi-enzyme system. This system includes the phage-encoded enzymes deoxy-2′-aminoadenylosuccinate synthetase (PurZ), deoxyadenosine triphosphate hydrolase (dATPase/DatZ), deoxyadenosine/deoxyguanosine triphosphate pyrophosphatase (DUF550/MazZ) and DNA polymerase (DpoZ). In this review, we provide a concise overview of the historical discovery on diversely modified nucleosides in bacteriophages, then we comprehensively summarize the research progress on multiple enzymes involved in the Z-genome biosynthetic pathway. Finally, the potential applications of the Z-genome and the enzymes in its biosynthetic pathway are discussed in order to provide reference for research in this field.
Key words： Z-genome; bacteriophage; DNA modification; 2-aminoadenine; biosynthetic pathway
Huiyu Chen, Suwen Zhao . Progress on Z genome biosynthetic pathway of bacteriophage[J]. Hereditas(Beijing), 2023 , 45(10) : 887 -903 . DOI: 10.16288/j.yczz.23-059
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