Massively parallel reporter assay: a novel technique for analyzing the regulation of gene expression
Received date: 2023-07-03
Revised date: 2023-09-30
Online published: 2023-10-07
Supported by
National Key Research and Development Program of China(2022YFF1000201);National Natural Science Foundation of China(31572394);China Agriculture Research System of MOF and MARA(CARS-41);Joint Guidance Project of Heilongjiang Natural Science Foundation(LH2021C036)
Massively parallel reporter assay (MPRA) is a high-throughput analysis method that can simultaneously investigate the activity of thousands of regulatory elements in the genome. MPRA introduces a uniquely identified barcode on a conventional luciferase reporter gene vector, sequences the DNA barcode before transfection and the mRNA barcode after transfection by next-generation sequencing technology, and uses the ratio of mRNA and DNA barcode reads to analyze the activity of cis-regulatory elements. Since MPRA was proposed, it has been widely used in the identification of genomic cis-regulatory elements and functional variants, the effect of post-transcriptional regulation on phenotypes and so on. In this review, we summarize the development history, basic principles, experimental procedures and statistical analysis methods of MPRA, and its applications in post-transcriptional regulation and cis-regulatory elements. It also provides prospects for its development and useful references for researchers in related fields to understand and apply MPRA.
Meng Yuan, Hui Li, Shouzhi Wang . Massively parallel reporter assay: a novel technique for analyzing the regulation of gene expression[J]. Hereditas(Beijing), 2023 , 45(10) : 859 -873 . DOI: 10.16288/j.yczz.23-180
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