Functional study of the soybean stamen-preferentially expressed gene <i>GmFLA22a</i> in regulating male fertility

Zhenlin Cao, Jinhong Li, Minhui Zhou, Manting Zhang, Ning Wang, Yifei Chen, Jiaxin Li, Qingsong Zhu, Wenjun Gong, Xuchen Yang, Xiaolong Fang, Jiaxian He, Meina Li

doi:10.16288/j.yczz.24-030

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Hereditas(Beijing) >

2024 , Vol. 46 >Issue 4: 333 - 345

DOI: https://doi.org/10.16288/j.yczz.24-030

Research Article

Functional study of the soybean stamen-preferentially expressed gene GmFLA22a in regulating male fertility

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Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design,Innovative Center of Molecular Genetics and Evolution, Guangzhou University, Guangzhou 510006, China

Received date: 2024-01-24

Revised date: 2024-03-07

Online published: 2024-03-29

Supported by

National Natural Science Foundation of China(32072084);National Science Fund Young Scholars(32101751)

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Abstract

China has a high dependence on soybean imports, yield increase at a faster rate is an urgent problem that need to be solved at present. The application of heterosis is one of the effective ways to significantly increase crop yield. In recent years, the development of an intelligent male sterility system based on recessive nuclear sterile genes has provided a potential solution for rapidly harnessing the heterosis in soybean. However, research on male sterility genes in soybean has been lagged behind. Based on transcriptome data of soybean floral organs in our research group, a soybean stamen-preferentially expressed gene GmFLA22a was identified. It encodes a fasciclin-like arabinogalactan protein with the FAS1 domain, and subcellular localization studies revealed that it may play roles in the endoplasmic reticulum. Take advantage of the gene editing technology, the Gmfla22a mutant was generated in this study. However, there was a significant reduction in the seed-setting rate in the mutant plants at the reproductive growth stage. The pollen viability and germination rate of Gmfla22a mutant plants showed no apparent abnormalities. Histological staining demonstrated that the release of pollen grains in the mutant plants was delayed and incomplete, which may due to the locule wall thickening in the anther development. This could be the reason of the reduced seed-setting rate in Gmfla22a mutants. In summary, our study has preliminarily revealed that GmFLA22a may be involved in regulating soybean male fertility. It provides crucial genetic materials for further uncovering its molecular function and gene resources and theoretical basis for the utilization of heterosis in soybean.

Key words： soybean; heterosis; male sterility; gene editing; reverse genetics

Cite this article

Zhenlin Cao, Jinhong Li, Minhui Zhou, Manting Zhang, Ning Wang, Yifei Chen, Jiaxin Li, Qingsong Zhu, Wenjun Gong, Xuchen Yang, Xiaolong Fang, Jiaxian He, Meina Li . Functional study of the soybean stamen-preferentially expressed gene GmFLA22a in regulating male fertility[J]. Hereditas(Beijing), 2024 , 46(4) : 333 -345 . DOI: 10.16288/j.yczz.24-030

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