A non-synonymous variant of C21R in the ABCG2 gene is significantly associated with brown eggshell color of Lueyang black-boned chicken
Received date: 2024-08-05
Revised date: 2024-09-27
Online published: 2024-11-19
Supported by
Key Research and Development Program of Shaanxi Province(2024NC-YBXM-089);Project of Department of Agriculture and Rural Affairs of Shaanxi Province(K3031223078);Project of Department of Agriculture and Rural Affairs of Shaanxi Province(K3031223151)
Eggshell colors have a significant effect on sale of eggs. The aim of this study is to identify sequence variants associated with color variation of chicken brown eggs in the ABCG2, a protoporphyrin transport-related gene. Three successive eggs were collected from each of 381 Lueyang black-boned hens at the 31 weeks old. Eggshell color was quantified via the L*a*b color space. g.18378442T>C (a non-synonymous mutation C21R), g.18383682A>G and g.18391548A>T were genotyped via the MassARRAY platform. The associations of genotypes with color indexes were tested by one-way ANOVA. The conservation of the C21R locus was analyzed by multiple alignment of 52 avian genomes from the UCSC database. We predicted 3D structures of ABCG2 with C21 and R21 by homology modelling using the MODELLER program to analyze effect of C21R on protein structures. Results showed that g.18378442T>C was significantly associated with L and b values. The L value (75.3±4.0) of CC was significantly lower than ones of CT (76.6±4.3) and TT (78.0±4.4) genotypes. The b value (16.2±3.5) of CC was significantly higher than ones of CT (15.7±3.6) and TT (15.7±3.6). g.18383682A>G was only associated with the L value (P<0.05). There was no any association between g.18391548A>T and three color indexes (P>0.05)。The C21 was completely conserved among 45 bird species. In contrast, the R21 was only present in two species of Psittaciformes. Structure comparisons showed that C21R potentially cause 468 structure alternations, of which 49.6% belong to hydrogen bond alternations, 38.8% for solvent accessible, 8.1% for positive φ angle and 3.5% for changes of secondary structures. In conclusion, this study identifies C21R as a molecular marker that is associated with color variation of chicken brown eggs and has a potential effect on protein structures. The CC genotype of C21R is associated with dark brown color and the TT for light brown.
Deyang Zhang, Wenchuan Zhou, Jiale Li, Zhepeng Wang . A non-synonymous variant of C21R in the ABCG2 gene is significantly associated with brown eggshell color of Lueyang black-boned chicken[J]. Hereditas(Beijing), 2024 , 46(12) : 1066 -1075 . DOI: 10.16288/j.yczz.24-225
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