Role of ppp6r3 in zebrafish gonadal differentiation and gametogenesis
Received date: 2025-04-02
Revised date: 2025-04-24
Online published: 2025-05-13
Supported by
National Key R&D Program of China(2022YFF0710500);National Key R&D Program of China(2024YFD2402300);STI 2030-Major Projects(2023ZD04069)
The gonad differentiation and gametogenesis of fish is regulated by various factors. Protein phosphatase (PP) have the function of catalyzing the dephosphorylation of proteins in organisms. As a member of the protein phosphatase family, protein phosphatase type 6 (PP6) plays an important role in gonadal development and gametogenesis. The role of ppp6r3, which encodes the regulatory subunit 3 of protein phosphatase 6, in gonadal differentiation and gametogenesis is still unclear. In this study, two zebrafish ppp6r3 mutant lines were constructed using CRISPR/Cas9 technology. It was found that the absence of ppp6r3 leads to the development of only male zebrafish, and these mutants are incapable of fertilizing wild-type eggs. Further investigations revealed that in the testes of ppp6r3 mutants, the transition of spermatocytes to mature sperm was blocked, disrupting spermatogenesis. In summary, this study established a ppp6r3 mutant model, exhibiting defects in gonadal differentiation and gametogenesis, which provides a new model for further research on the mechanisms by which Ppp6r3 regulates germ cell proliferation, differentiation, and sex determination.
Key words： Ppp6r3; zebrafish; gonadal differentiation; gametogenesis
Yunhai Gao, Jiajie Deng, Xiao Xiao, Luyuan Pan, Mudan He, Yunbin Zhang . Role of ppp6r3 in zebrafish gonadal differentiation and gametogenesis[J]. Hereditas(Beijing), 2025 , 47(9) : 1023 -1031 . DOI: 10.16288/j.yczz.25-093
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