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HEREDITAS ›› 2006, Vol. 28 ›› Issue (11): 1371-1375.doi: 10.1360/yc-006-1371

• 研究报告 • Previous Articles     Next Articles

Analysis of POU1F1 Gene Polymorphisms in Qinchuan Cattle and Chinese Holstein Cattle

YAN Lin-Jun1; LIU Bo2; FANG Xin-Tang1; CHEN Hong1,2; ZHANG Run-Feng2;
BAO Bin1; ZHANG Hai-Jun1
  

  1. 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Animal Science
    and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China
  • Received:2005-12-30 Revised:2006-02-27 Online:2006-11-05 Published:2006-11-05
  • Contact: CHEN Hong

Abstract:

PCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated HinfⅠ polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.232/0.768 and 0.132/0.868, respectively. The frequencies of three genotypes AA, AB and BB were 0.030/0.403/0.567 and 0.007/0.251/0.742, respectively. Qinchuan cattle population was at Hardy-Weinberg equilibrium at this locus, but Chinese Holstein cattle population was not. The gene heterozygosity/effective allele gene number/Shannon information entropy /polymorphism information content of Qinchuan cattle and Chinese Holstein cattle populations were listed for 0.356/1.553/0.541/0.292 and 0.229/1.297/0.390/0.203, respectively. All indices were higher in the Qinchuan cattle population.

CLC Number: