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HEREDITAS ›› 2006, Vol. 28 ›› Issue (12): 1580-1580~1584.doi: 10.1360/yc-006-1580

• 研究报告 • Previous Articles     Next Articles

Expression of Rabbit Neutrophile Peptide-1 in Nitrate Reductase- deficient Mutant of Chlorella ellipsoidea

ZHANG Xiao-Yu1,2, WANG Peng1, ZHAO Shi-Min1, LI Xia2, SHEN Xin2, SUN Yong-Ru1, CHU Cheng-Cai1, WANG Yi-Qin1
  

  1. (1. State Key Laboratory of Plant Genomics and National Plant Gene Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 2. College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China)
  • Received:2006-03-10 Revised:2006-05-12 Online:2006-12-10 Published:2006-12-10
  • Contact: WANG Yi-Qin

Abstract:

Application of transgenic Cholrella as bioreactor to express rabbit neutrophile pepetide-1 (NP-1) shows great practical value. In this paper, an NP-1 expression vector containing two selective marker genes NPTII and nitrate reductase gene was constructed. The NP-1 gene was transformed into the nitrate reductase-deficient mutant nrm-4 of Chlorella ellipsoidea via electroporation, and the transgenic alga expressed the active NP-1 were obtained.

CLC Number: