Abstract:

Erwinia carotovora subsp. carotovora CSDS001 elicits hypersensitive reaction (HR) in tobacco. From the genomic libraries of Erwinia carotovora subsp. carotovora CSDS001, the hrpN_CSDS001 gene (Genebank number AY939927), was isolated. The hrpN_CSDS001 fusion protein was produced in Escherichia coli, and was used to induce HR by injecting into tobacco. We further examined the global regulation of Arabidopsis thaliana genes in response to Harpin_CSDS001 at a concentration of 30 mg/mL. We indicated that 912、1787、2393、1833 and 1,755 genes that were regulated significantly (log ratio ≤-1 or ≥1) at 3 h、12 h、24 h、36 h and 48 h respectively after the treatment. Analysis of some transcription factors (TF) showed that 13 TF families responded to Harpin_CSDS001including ZIM、BES1、TCP、C2C2、AP2/EREBP、WRKY、

bHLH、bZIP、GARP、MYB、NAC、HB、C2H2. These families mainly function in biological processes of plant defense、photosynthesis、development and flowering.