CRISPR/Cas9系统在培育抗病毒植物新种质中的应用

doi:10.16288/j.yczz.16-134

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Hereditas(Beijing) ›› 2016, Vol. 38 ›› Issue (9): 811-820.doi: 10.16288/j.yczz.16-134

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Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm

Daowei Zhang¹, Chaofan Zhang^{1, 2}, Fang Dong², Yanlan Huang¹, Ya Zhang¹, Hong Zhou¹

1. Hunan Crop Research Institute, Changsha 410125, China;
2. Longping Branch of Graduate School of Central South University, Changsha 410125, China

Received:2016-04-18 Online:2016-09-20 Published:2016-09-20
Supported by:
[Supported by China Agriculture Research System of Sweet Potato (No; CARS-11-C-16) and Agriculture Research System of Upland Crops in Hunan Province (the Post of Prevention and Control of Pest and Disease)]

Abstract

Abstract: With the development and improvement of CRISPR/Cas9 system in genomic editing technology, the system has been applied to the prevention and control of animal viral infectious diseases, which has made considerable achievements. It has also been applied to the study of highly efficient gene targeting editing in plant virus genomes. The CRISPR/Cas9-mediated targeted gene modification has not only achieved the genome editing of plant DNA virus, but also showed the genome editing potential of plant RNA virus. In addition, the CRISPR/Cas9 system functions at the gene transcriptional and post-transcriptional level, indicating that the system could regulate the replication of plant viruses through different ways. Compared with other plant viral disease control strategies, this system is more accurate in genome editing, more stable in gene expression regulation, and has broader spectrum of resistance to virus disease. In this review, we summarized the advantages, main problems and development tendency of CRISPR/cas9 system in breeding of new antiviral plant germplasms.

Key words: CRISPR/Cas9 System, virus disease prevention and control, genome targeting modification

Daowei Zhang, Chaofan Zhang, Fang Dong, Yanlan Huang, Ya Zhang, Hong Zhou. Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm[J]. Hereditas(Beijing), 2016, 38(9): 811-820.

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Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm

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