单细胞基因组测序技术新进展及其在生物医学中的应用

doi:10.16288/j.yczz.20-363

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (2): 108-117.doi: 10.16288/j.yczz.20-363

• Review • Previous Articles Next Articles

Advances in single-cell whole genome sequencing technology and its application in biomedicine

Zhuo Wang¹(), Xiaohan Shen¹, Qihui Shi¹()

¹ Institutes of Biomedical Sciences, Fudan University, Shanghai 201100, China

Received:2020-12-01 Online:2021-02-16 Published:2021-01-22
Supported by:
China Postdoctoral Science Foundation Grant(2019M651377);Shanghai “Super Postdoctoral Fellow” Program(2018-2020)

Abstract

Abstract:

The advent and development of single-cell whole-genome sequencing (scWGS) technology has shed lights on the genomic heterogeneities within biosamples at the single-cell resolution. The technology is particularly well-established in the recent decade and witnesses a variety of clinical applications, such as circulating tumor cell (CTC) detection and preimplantation genetic diagnosis/screening (PGD/PGS). In this review, we summarize the latest practical breakthroughs of scWGS in the field of biomedicine, with the hope of providing a guideline to apply single-cell genomic sequencing in clinical researches.

Key words: single cell whole genome, high throughput sequencing, heterogeneity, circulating tumor cell

Zhuo Wang, Xiaohan Shen, Qihui Shi. Advances in single-cell whole genome sequencing technology and its application in biomedicine[J]. Hereditas(Beijing), 2021, 43(2): 108-117.

Figures/Tables 2

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Advances in single-cell whole genome sequencing technology and its application in biomedicine

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