江苏省植物细胞遗传学研究回顾与展望

doi:10.16288/j.yczz.20-448

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (5): 397-424.doi: 10.16288/j.yczz.20-448

• Review • Previous Articles Next Articles

Research progress of plant cytogenetics in Jiangsu province

Haiyan Wang¹, Zhiyun Gong², Jiafu Jiang¹, Baoliang Zhou¹, Qunfeng Lou¹, Qinghe Cao³, Mengli Xi⁴, Peidu Chen¹, Minghong Gu², Tianzhen Zhang⁶, Fadi Chen¹, Jinfeng Chen¹, Zongyun Li⁵, Xiue Wang¹

1. State Key Laboratory of Crop Genetics & Germplasm Enhancement/JCIC-MCP, Nanjing Agricultural University, College of Agriculture, Nanjing 201195, China;
2. Jiangsu Key Laboratory of Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of Ministry of Education/, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou 225009, China
3.Xuzhou Institute of Agricultural Sciences of the Xuhuai District, Jiangsu, Xuzhou 221131, China
4.Nanjing Forestry University, Nanjing 210037, China
5.School of Life Sciences Jiangsu Normal University, Xuzhou 221116, China
6.College of Agriculture and Biotechnology Zhejiang University, Hangzhou 310058, China

Received:2020-12-29 Revised:2021-02-25 Online:2021-05-20 Published:2021-03-16
Supported by:
supported by the National Key Research and Development Program of China No. 2016YFD0102001(2016YFD0102001);International Cooperation and Exchange Programme of the National Natural Science Foundation of China No(31661143005);the National Natural Science Foundation of China No(31771782)

Abstract

Abstract:

Cytogenetics was established based on the “Chromosome theory of inheritance”, proposed by Boveri and Sutton and evidenced by Morgan’s lab in early stage of the 20 ^th centrary. With rapid development of related research areas, especially molecular genetics, cytogenetics developed from traditional into a new era, molecular cytogenetics in late 1960s. Featured by an established technique named DNA in situ hybridization (ISH), molecular cytogenetics has been applied in various research areas. ISH provids vivid and straightforward figures showing the virtual presence of DNA, RNA or proteins. In combination with genomics and cell biology tools, ISH and derived techniques have been widely used in studies of the origin, evolution, domestication of human, animal and plant, as well as wide hybridization and chromosome engineering. The physical location and order of DNA sequences revealed by ISH enables the detection of chromosomal re-arrangments among related species and gaps of assembled genome sequences. In addition, ISH using RNA or protein probes can reveal the location and quantification of transcripted RNA or translated protein. Since the 1970s, scientists from universities or institutes belonging to the Jiangsu Society of Genetics have initiated cytogenetics researches using various plant species. In recent years, research platforms for molecular cytogenetics have also been well established in Nanjing Agricultural University, Yangzhou University, Nanjing Forestry University, Jiangsu Xuhuai Academy of Agricultural Sciences, and Jiangsu Normal University. The application of molecular cytogenetics in plant evolution, wide hybridization, chromosome engineering, chromosome biology, genomics has been successful. Significant progresses have been achieved, both in basic and applied researches. In this paper, we will review main research progresses of plant cytogenetics in Jiangsu province, and discuss the potential development of this research area.

Key words: Jiangsu Genetics Society, molecular and cytogenetics, DNA in situ hybridization, chromosome engineering, geneomics

Haiyan Wang, Zhiyun Gong, Jiafu Jiang, Baoliang Zhou, Qunfeng Lou, Qinghe Cao, Mengli Xi, Peidu Chen, Minghong Gu, Tianzhen Zhang, Fadi Chen, Jinfeng Chen, Zongyun Li, Xiue Wang. Research progress of plant cytogenetics in Jiangsu province[J]. Hereditas(Beijing), 2021, 43(5): 397-424.

Figures/Tables 5

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Table 1

References 199

[1]	Gill BS, Friebe B, Endo TR . Standard karyotype and nomenclature system for description of chromosome bands and structural aberrations in wheat (Triticum aestivum). Genome, 1991,34(5):830-839.
[2]	Rayburn AL, Gill BS . Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered, 1985,76(2):78-81.
[3]	Rayburn AL, Gill BS . Molecular identification of the D-genome chromosomes of wheat. J Hered, 1986,77(4):253-255.
[4]	Rayburn AL, Gill BS . Molecular analysis of the D-genome of the Triticeae. Theor Appl Genet, 1987,73(3):385-388.
[5]	Mukai Y, Nakahara Y, Yamamoto M . Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes. Genome, 1993,36(3):489-494.
[6]	Zhang W, Zhang RQ, Feng YG, Bie TD, Chen PD . Distribution of highly repeated DNA sequences in Haynaldia villosa and its application in the identification of alien chromatin. Chin Sci Bull, 2013,58(8):890-897.
[7]	Dolezel J, Kubalaková M, Paux E, Bartos J, Feuillet C . Chromosome-based genomics in the cereals. Chromosome Res, 2007,15(1):51-66.
[8]	Doležel J, Vrána J, Cápal P, Kubaláková M, Burešová V, Simková H . Advances in plant chromosome genomics. Biotechnol Adv, 2014,32(1):122-136.
[9]	Kubaláková M, Valárik M, Barto J, Vrána J, Cíhalíkova J, Molnár-Láng M, Dolezel J . Analysis and sorting of rye (Secale Cereale L.) chromosomes using flow cytometry. Genome, 2003,46(5):893-905.
[10]	Hernandez P, Martis M, Dorado G, Pfeifer M, Gálvez S, Schaaf S, Jouve N, Šimková H, Valárik M, Doležel J, Mayer KFX . Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content. Plant J, 2012,69(3):377-386.
[11]	Chen PD, Gill BS . The Origin of chromosome 4A and genomes B and G of tetraploid wheats. Acta Agron Sin, 1984,10(3):146-153.
	陈佩度 , Gill BS. 四倍体小麦染色体4A和B, G染色体组的起源. 作物学报, 1984,10(3):146-153.
[12]	Liu DJ, Chen PD . N-banding in Haynaldia villosa and Triticum durum-H. villosa amphidiploid. Acta Genet Sin, 1984,11(2):106-108.
	刘大钧, 陈佩度 . 簇毛麦和硬粒小麦-簇毛麦双二倍体的染色体N-分带. 遗传学报, 1984,11(2):106-108.
[13]	Dong FG, Chen PD, Liu DJ . An improved C-banding technique for Haynaldia villosa chromosomes. Acta Genet Sin, 1991,18(6):525-528.
	董凤高, 陈佩度, 刘大钧 . 簇毛麦染色体的改良C-分带. 遗传学报, 1991,18(6):525-528.
[14]	Yan Y, Liu DJ . Production and cytogenetics of intergeneric hybrid between Roegneria ciliaris and Triticum aestivum. Sci Agric Sin, 1987,20(6):17-21.
	颜旸, 刘大钧 . 纤毛鹅观草与普通小麦属间杂种的产生及其细胞遗传学研究. 中国农业科学, 1987,20(6):17-21.
[15]	Weng YQ, Liu DJ . Morphology, scab resistance and cytogenetics of inter generic hybrids of Triticum aestivum L. with Roegneria C. Koch (Agropyron) species. Sci Agric Sin, 1989,22(5):1-7.
	翁益群, 刘大钧 . 鹅观草与普通小麦属间杂种F₁的形态—赤霉病抗性与细胞遗传学研究. 中国农业科学, 1989,22(5):1-7.
[16]	Jiang JM, Liu DJ . Morphology and cytogenetics of intergeneric hybrid between Roegneria kamoji and Hordeum vulgare. Acta Genet Sin, 1990: 373-376.
	蒋继明, 刘大钧 . 鹅观草与大麦属间杂种的形态和细胞遗传学研究. 遗传学报, 1990: 373-376.
[17]	Chen PD, Wang ZT, Wang SL, Wang L, Wang YZ, Liu DJ . Transfer of useful germplasm form Leymus racemosus Lam. to common wheat. III. Development of addition lines with wheat scab resistance. Acta Genet Sinica, 1995,22(3):206-210.
[18]	Liu DJ, Chen PD, Wu YL, Wang YN, Qiu BX, Wang SL . Triticum durum-Haynaldia villosa amphidiploid. Acta Agron Sin, 1986,12(3):155-162.
	刘大钧, 陈佩度, 吴沛良, 王耀南, 邱伯行, 王苏玲 . 硬粒小麦-簇毛麦双二倍体. 作物学报, 1986,12(3):155-162.
[19]	Pei GZ, Chen PD, Liu DJ . A cytogenetic analysis of some powdery mildew resistant strains of the hybrid progeny between wheat and H. villosa. J Nanjing Agric Univ, 1986,9(1):1-9.
	裴广铮, 陈佩度, 刘大钧 . 小麦与簇毛麦杂种后代抗白粉病株系的细胞遗传学分析. 南京农业大学学报, 1986,9(1):1-9.
[20]	Chen PD, Qi LL, Zhou B, Zhang SZ, Liu DJ . Development and molecular cytogenetic analysis of wheat- Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew. Theor Appl Genet, 1995,91(6-7):1125-1128.
[21]	Li WL, Chen PD, Qi LL, Liu DJ . Isolation, characterization and application of a species-specific repeated sequence from Haynaldia villosa. Theor Appl Genet, 1995,90(3-4):526-533.
[22]	Xu LY, Yang JX, Xuan P, Liu DJ, Yang SH . Analysis the relative relationship of wheat- Thinopyron using the C-banding of pollen mother cell and ditelosomics. Sci Agric Sin, 1996,29(4):23-28.
	徐利远, 杨吉秀, 宣朴, 刘大钧, 杨世湖 . 用花粉母细胞分带和端体研究小麦与偃麦草的亲缘关系. 中国农业科学, 1996,29(4):23-28.
[23]	Liu WX, Chen PD, Liu DJ . Development of Triticum aestivum-Leymus racemosus translocation lines by irradiating adult plants at meiosis. Acta Bot Sin, 1999,41(5):463-467.
	刘文轩, 陈佩度, 刘大钧 . 利用减数分裂期成株电离辐射选育小麦-大赖草易位系的研究. 植物学报, 1999,41(5):463-467.
[24]	Qi ZJ, Liu DJ, Chen PD . Development and identification of T. aestivum-S. cereale-H. villosa double translocation line 1RS·1BL, 6VS·6AL via chromosome C-banding and dual color FISH. Acta Genet Sin, 2001,28(3):267-273.
	亓增军, 刘大钧, 陈佩度 . 利用染色体C-分带和双色荧光原位杂交技术鉴定普通小麦-黑麦-簇毛麦双重易位系1RS·1BL,·6VS·6AL. 遗传学报, 2001,28(3):267-273.
[25]	Chen FD, Chen PD, Wang SL . Development of wheat-alien lines with added Leymus racemosus chromosomes and 6VS/6AL translocation chromosomes. Acta Bot Sin, 2001,43(4):359-363.
	陈发棣, 陈佩度, 王苏玲 . 普通小麦-大赖草-簇毛麦异附加、易位系的选育和鉴定. 植物学报, 2001,43(4):359-363.
[26]	Wang XE, Chen PD, Liu DJ, Zhang P, Zhou B, Friebe B, Gill BS . Molecular cytogenetic characterization of Roegneria ciliaris chromosome additions in common wheat. Theor Appl Genet, 2001,102:651-657.
[27]	Wang XE, Chen PD, Zhou B, Yuan JH, Liu WX, Gill BS, Liu DJ . RFLP analysis of Wheat- L. racemosus translocation lines. Acta Genet Sin, 2001,28(12):1142-1150.
	王秀娥, 陈佩度, 周波, 袁建华, 刘文轩, Gill BS, 刘大钧. 小麦-大赖草易位系的RFLP分析. 遗传学报, 2001,28(12):1142-1150.
[28]	Zhuang LF, Qi ZJ, Ying J, Chen PD, Liu DJ . Development and identification of a set of Triticum aestivum-Thinopyrum bessarabicum disomic alien addition lines. Acta Genet Sin, 2003,30(10):919-925.
	庄丽芳, 亓增军, 英加, 陈佩度, 刘大钧 . 普通小麦-百萨偃麦草二体异附加系的选育与鉴定. 遗传学报, 2003,30(10):919-925.
[29]	Chen PD, Liu WX, Yuan JH, Wang XE, Zhou B, Wang SL, Zhang SZ, Feng YG, Yang BJ, Liu GX, Liu DJ, Qi LL, Zhang P, Friebe B, Gill BS . Development and characterization of wheat- Leymus racemosus translocation lines with resistance to Fusarium head blight. Theor Appl Genet, 2005,111(5):941-948.
[30]	Du P, Zhuang LF, Wang YZ, Yuan L, Wang Q, Wang DR, Dawadondup , Tan LJ, Shen J, Xu HB, Zhao H, Chu CG, Qi ZJ,. Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes. Genome, 2017,60(2):93-103.
[31]	Wang YZ . Development and characterization of small segment translocations of Thinopyrum bessarabicum and cytological mapping of interest genes[Dissertation]. Nanjing Agricultural University, 2013.
	王艳芝 . 百萨偃麦草染色体小片段易位的创制、鉴定与基因定位分析[学位论文]. 南京农业大学, 2013.
[32]	Yang J, Liu ZT, Chen JY, Wang YZ, Zhuang LF, Qi ZJ . Accurate characterization of wheat- Thinopyrum bessarabicum alien chromosome translocations using oligonucleotide multiplex painting combined with genomic in situ hybridization and molecular marker analysis. J Trit Crops, 2018,38(3):253-261.
	杨骄, 刘志涛, 陈健泳, 王艳芝, 庄丽芳, 亓增军 . 寡核苷酸探针套涂染结合基因组原位杂交和分子标记分析准确鉴定小麦-百萨偃麦草异源易位系的研究. 麦类作物学报, 2018,38(3):253-261.
[33]	Wang DR, Du P, Pei ZY, Zhuang LF, Qi ZJ . Development and application of high resolution karyotypes of wheat “Chinese Spring” aneuploids. Acta Agron Sin, 2017,43(11):1575-1587.
	王丹蕊, 杜培, 裴自友, 庄丽芳, 亓增军 . 基于寡核苷酸探针套painting的小麦“中国春”非整倍体高清核型及应用. 作物学报, 2017,43(11):1575-1587.
[34]	Huang XY, Zhu MQ, Zhuang LF, Zhang SY, Wang JJ, Chen XJ, Wang DR, Chen JY, Bao YG, Guo J, Zhang JL, Feng YG, Chu CG, Du P, Qi ZJ, Wang HG, Chen PD . Structural chromosome rearrangements and polymorphisms identified in Chinese wheat cultivars by high- resolution multiplex oligonucleotide FISH. Theor Appl Genet, 2018,131(9):1967-1986.
[35]	Guo JT, Lei YH, Zhang HT, Song DH, Liu X, Cao ZL, Chu CG, Zhuang LF, Qi ZJ . Frequent variations in tandem repeats pSc200 and pSc119.2 cause rapid chromosome evolution of open-pollinated rye. Mol Breeding, 2019,39:133.
[36]	Sun HJ, Song JJ, Lei J, Song XY, Dai KL, Xiao J, Yuan CX, An SM, Wang HY, Wang XE . Construction and application of oligo-based FISH karyotype of Haynaldia villosa. J Genet Genomics, 2018,45(8):463-466.
[37]	Xiao J, Dai KL, Fu L, Vrána J, Kubaláková M, Wan WT, Sun HJ, Zhao J, Yu CY, Wu YF, Abrouk M, Wang HY, Doležel J, Wang XE . Sequencing flow-sorted short arm of Haynaldia villosa chromosome 4V provides insights into its molecular structure and virtual gene order. BMC Genomics, 2017,18(1):791.
[38]	Lei J, Zhou JW, Sun HJ, Wan WT, Xiao J, Yuan CX, Karafiátová M, Doležel J, Wang HY, Wang XE . Development of oligonucleotide probes for FISH karyotyping in Haynaldia villosa, a wild relative of common wheat. Crop J, 2020,8(4):676-681.
[39]	Song XY, Song RR, Zhou JW, Yan WK, Zhang T, Sun HJ, Xiao J, Wu YF, Xi ML, Lou QF, Wang HY, Wang XE . Development and application of oligonucleotide- based chromosome painting for chromosome 4D of Triticum aestivum L. Chromosome Res. 2020,28(2):171-182.
[40]	Chen PD, You CF, Hu Y, Chen SW, Zhou B, Cao AZ, Wang XE . Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat. Mol Breeding, 2013,31:477-484.
[41]	Yang XM, Cao AZ, Sun YL, Chen PD . Tracing the location of powdery mildew resistance-related gene Stpk-V by FISH with a TAC clone in Triticum aestivum- Haynaldia villosa alien chromosome lines. Chin Sci Bull, 2013,58:4084-4091.
[42]	Cao AZ, Xing LP, Wang XY, Yang XM, Wang W, Sun YL, Qian C, Ni JL, Chen YP, Liu DJ, Wang XE, Chen PD . Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA, 2011,108(19):7727-7732.
[43]	Xing LP, Hu P, Liu JQ, Witek K, Zhou S, Xu JF, Zhou WH, Gao L, Huang ZP, Zhang RQ, Wang XE, Chen PD, Wang HY, Jones JDG, Karafiátová M, Vrána J, Bartoš J, Doležel J, Tian YC, Wu YF, Cao AZ . Pm21 from Haynaldia villosa encodes a CC-NBS-LRR protein conferring powdery mildew resistance in wheat. Mol Plant, 2018,11(6):874-878.
[44]	Bao WK . Breeding of octoploid triticale. Acta Genet Sin, 1974,1(1):36-49.
	鲍文奎 . 八倍体小黑麦的育种. 遗传学报, 1974,1(1):36-49.
[45]	Li JY. Selected some academic articles of Zhensheng. 2007, Science Press.
	李家洋 . 《李振声论文选集》. 2007, 科学出版社.
[46]	Hao S, He MY, Xu ZY, Zhang CS, Zou MQ, Zhang H, Sun P . Cytogenetic studies on five intermediate forms of progeny of Triticum aestivum × Agropyron glaucum. J Integr Plant Biol, 1979,21(3):259-261.
	郝水, 何孟元, 徐宗尧, 张传善, 邹明谦, 张汉, 孙平 . 小麦×天蓝冰草后代五个中间类型的细胞遗传学研究. 植物学报, 1979,21(3):259-261.
[47]	Li JL, Xu XL, Zhao LX, Xue X . The cell inheritance of Triticale by induced mutation. Sci Agric Sin, 1981,1:12-21.
	李集临, 徐香玲, 赵连喜, 薛玺 . 小黑麦(Triticale)诱发突变的细胞遗传. 中国农业科学, 1981,1:12-21.
[48]	Sun SC . The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sin, 1981,7(l):51-58.
	孙善澄 . 小偃麦新品种与中间类型的选育途径、程序和方法. 作物学报, 1981,7(l):51-58.
[49]	Chen PD, Liu DJ . Cytogenetic studies of hybrid progenies between Triticum aestivum and Haynaldia villosa. J Nanjing Agric Univ, 1982,4(4):1-16.
	陈佩度, 刘大钧 . 普通小麦与簇毛麦杂种后代的细胞遗传学研究. 南京农学院学报, 1982,4(4):1-16.
[50]	Chen PD, Zhou B, Qi LL, Liu DJ . Identification of wheat- Haynaldia villosa amphiploid, addition, substitution and translocation lines by in situ hybridization using biotin-labelled genomic DNA as a Probe. Acta Genet Sin, 1995,22(5):380-386.
	陈佩度, 周波, 齐莉莉, 刘大钧 . 用分子原位杂交(GISH)鉴定小麦-簇毛麦双倍体、附加系、代换系和易位系. 遗传学报, 1995,22(5):380-386.
[51]	Chen YP, Wang HZ, Cao AZ, Wang CM, Chen PD . Cloning of a resistance gene analog from wheat and development of a codominant PCR marker for Pm21. J Integr Plant Biol, 2006,48(6):715-721.
[52]	Cao AZ, Wang XE, Chen YP, Zou XW, Chen PD . A sequence-specific PCR marker linked with Pm21 distinguishes chromosomes 6AS, 6BS, 6DS of Triticum aestivum and 6VS of Haynaldia villosa. Plant Breeding, 2006,125(3):201-205.
[53]	Wang CM, Bie TD, Chen QZ, Cao AZ, Chen PD . Development and application of molecular markers specific to chromosome 6VS of Haynaldia villosa. Acta Agron Sin, 2007,33(10):1595-1600.
	王春梅, 别同德, 陈全战, 曹爱忠, 陈佩度 . 簇毛麦6V染色体短臂特异分子标记的开发和应用. 作物学报, 2007,33(10):1595-1600.
[54]	Zhao RH, Wang HY, Jia Q, Xiao J, Yuan CX, Zhang YJ, Hu QS, Wang XE . Development of EST-PCR markers for the chromosome 4V of Haynaldia villosa and their application in identification of 4V chromosome structural aberrants. J Integr Agricul, 2014,13(2):282-289.
[55]	Wang HY, Dai KL, Xiao J, Yuan CX, Zhao RH, Doležel J, Wu YF, Cao AZ, Chen PD, Zhang SZ, Wang XE . Development of intron targeting (It) markers specific for chromosome arm 4VS of Haynaldia Villosa by chromosome sorting and next-generation sequencing. BMC Genomics, 2017,18(1):167.
[56]	Zhang XD, Wei X, Xiao J, Yuan CX, Wu YF, Cao AZ, Xing LP, Chen PD, Zhang SZ, Wang XE, Wang HY . Whole genome development of intron targeting (IT) markers specific for Dasypyrum villosum chromosomes based on next-generation sequencing technology. Mol Breeding, 2017,37:115.
[57]	Sun HJ, Song JJ, Xiao J, Xu T, Wei X, Yuan CX, Cao AZ, Xing LP, Wang HY, Wang XE . Development of EST-PCR markers specific to the long arm of chromosome 6V of Dasypyrum villosum. J Integr Agricul, 2018,17(8):1720-1726.
[58]	Qi LL, Chen PD, Liu DJ, Zhou B, Zhang SZ . New resource of wheat-Haynaldia villosa translocation resistant to wheat powdery mildew. Crop Var Resour, 1994,2:52-53.
	齐莉莉, 陈佩度, 刘大钧, 周波, 张守忠 . 小麦白粉病的新抗源—普通小麦簇毛麦易位系. 作物品种资源, 1994,2:52-53.
[59]	Bie TD, Cao YP, Chen PD . Mass production of intergeneric chromosomal translocations through pollen irradiation of Triticum durum-Haynaldia villosa amphiploid. J Integr Plant Biol, 2007,49(11):1619-1626.
[60]	Chen SW, Chen PD, Wang XE . Inducement of chromosome translocation with small alien segments by irradiating mature female gametes of the whole arm translocation line. Sci China C Life Sci, 2008,51(4):346-352.
[61]	Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin Y, Gill BS . A novel robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet, 2011,123(1):159-167.
[62]	Zhang RQ, Feng YG, Li HF, Yuan HX, Dai JL, Cao AZ, Xing LP, Li HL . Cereal cyst nematode resistance gene CreV effective against Heterodera filipjevi transferred from chromosome 6VL of Dasypyrum villosum to bread wheat. Mol Breeding, 2016,36:122.
[63]	Zhang RQ, Sun BX, Chen J, Cao AZ, Xing LP, Feng YG, Lan CX, Chen PD . Pm55, a developmental-stage and tissue-specific powdery mildew resistance gene introgressed from Dasypyrum villosum into common wheat. Theor Appl Genet, 2016,129(10):1975-1984.
[64]	Zhao RH, Wang HY, Xiao J, Bie TD, Cheng SH, Jia Q, Yuan CX, Zhang RQ, Cao AZ, Chen PD, Wang XE . Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa. Theor Appl Genet, 2013,126(12):2921-2930.
[65]	Dai KL, Zhao RH, Shi MM, Xiao J, Yu ZY, Jia Q, Wang ZK, Yuan CX, Sun HJ, Cao AZ, Zhang RQ, Chen PD, Li YB, Wang HY, Wang XE . Dissection and cytological mapping of chromosome arm 4VS by the development of wheat- Haynaldia villosa structural aberration library. Theor Appl Genet, 2020,133(1):217-226.
[66]	Matsumoto TE . International Rice Genome Sequencing Project. The map-based sequence of the rice genome. Nature, 2005,436:793-800.
[67]	Cheng ZK, Gu MH . Karyotype analysis for pachytene chromosome of indica, Japonica Rice and their hybrid. Acta Genet Sin, 1994,21(5):385-392.
	程祝宽, 顾铭洪 . 籼、粳稻及其杂种粗线期的核型分析. 遗传学报, 1994,21(5):385-392.
[68]	Cheng Z, Yan H, Yu H, Tang S, Jiang J, Gu M, Zhu L . Development and applications of a complete set of rice telotrisomics. Genetics, 2001,157(1):361-368.
[69]	Tang XM, Bao WD, Zhang WL, Cheng ZK . Identification of chromosomes from multiple rice genomes using a universal molecular cytogenetic marker system. J Integr Plant Biol, 2007,49(6):953-960.
[70]	Liu XY, Sun S, Wu Y, Zhou Y, Gu SW, Yu HX, Yi CD, Gu MH, Jiang JM, Liu B, Zhang T, Gong ZY . Dual- color oligo-FISH can reveal chromosomal variations and evolution in Oryza species. Plant J, 2020,101(1):112-121.
[71]	Cheng Z, Buell CR, Wing RA, Gu M, Jiang J . Toward a cytological characterization of the rice genome. Genome Res, 2001,11(12):2133-2141.
[72]	Nakayama S . Molecular cytological diversity in cultivated rice Oryza sativa subspecies japonica and indica. Breeding Sci, 2005,55:425-430.
[73]	Ohtsubo H, Umeda M , Ohtsubo E. Organization of DNA sequences highly repeated in tandem in rice genomes. Jpn J Genet, 1991,66(3):241-254.
[74]	Fan CZ, Zhang Y, Yu Y, Rounsley S, Long MY, Wing RA . The subtelomere of Oryza sativa chromosome 3 short arm as a hot bed of new gene origination in rice. Mol Plant, 2008,1(5):839-850.
[75]	Jiang JM, Birchler JA, Parrott WA, Dawe RK . A molecular view of plant centromeres. Trends Plant Sci, 2003,8(12):570-575.
[76]	Dong F, Miller JT, Jackson SA, Wang GL, Ronald PC, Jiang J . Rice (Oryza sativa) centromeric regions consist of complex DNA. Proc Natl Acad Sci USA, 1998,95(14):8135-8140.
[77]	Cheng ZK, Dong FG, Langdon T, Ouyang S, Buell CR, Gu MH, Blattner FR, Jiang JM . Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell, 2002,14(8):1691-1704.
[78]	Wu ZG, Fang DM, Yang R, Gao F, An XY, Zhuo XX, Li YF, Yi CD, Zhang T, Liang CZ, Cui P, Cheng ZK, Luo Q . De novo genome assembly of Oryza granulata reveals rapid genome expansion and adaptive evolution. Commun Biol, 2018,1:84.
[79]	Gao DY, Gill N, Kim HR, Walling JG, Zhang WL, Fan CZ, Yu Y, Ma JX, SanMiguel P, Jiang N, Cheng ZK, Wing RA, Jiang JM, Jackson SA. A lineage-specific centromere retrotransposon in Oryza brachyantha. Plant J, 2009,60(5):820-831.
[80]	Gong ZY, Xue C, Liu XX, Zhang ML, Zhou Y, Yu HX, Gu MH . Centromere inactivation in a dicentric rice chromosome during sexual reproduction. Chin Sci Bull, 2013,58(36):4602-4607.
[81]	Gong ZY, Yu HX, Huang J, Yi CD, Gu MH . Unstable transmission of rice chromosomes without functional centromeric repeats in asexual propagation. Chromosome Res, 2009,17(7):863-872.
[82]	Zhang H, Dawe RK . Total centromere size and genome size are strongly correlated in ten grass species. Chromosome Res, 2012,20(4):403-412.
[83]	Yan HH, Kikuchi S, Neumann P, Zhang WL, Wu YF, Chen F, Jiang JM . Genome-wide mapping of cytosine methylation revealed dynamic DNA methylation patterns associated with genes and centromeres in rice. Plant J, 2010,63(3):353-365.
[84]	Wu YF, Kikuchi S, Yan HH, Zhang WL, Rosenbaum H, Iniguez AL, Jiang JM . Euchromatic subdomains in rice centromeres are associated with genes and transcription. Plant Cell, 2011,23(11):4054-4064.
[85]	He RF, Wang YY, Shi ZY, Ren X, Zhu LL, Weng QM, He GC . Construction of a genomic library of wild rice and agrobacterium-mediated transformation of large insert DNA linked to BPH resistance locus. Gene, 2003,321:113-121.
[86]	Tan GX, Ren X, Weng QM, Shi ZY, Zhu LL, He GC . Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genet Sin, 2004,31(7):724-729.
[87]	Huang Z, He G, Shu L, Li X, Zhang Q . Identification and mapping of two brown planthopper resistance genes in rice. Theor Appl Genet, 2001,102:929-934.
[88]	Yan HH, Xiong ZM, Min SK, Hu HY, Zhang ZT, Tian SL, Fu Q . The production and cytogenetical studies of Oryza sativa-Oryza eichingeri amphiploid. Acta Genet Sin, 1997,24(1):30-35.
	颜辉煌, 熊振民, 闵绍楷, 胡慧英, 张志涛, 田淑兰, 傅强 . 栽培稻-紧穗野生稻双二倍体的产生及其细胞遗传学研究. 遗传学报, 1997,24(1):30-35.
[89]	Sebastian P, Schaefer H, Telford IRH, Renner SS . Cucumber (Cucumis sativus) and melon (C. melo) have numerous wild relatives in Asia and Australia, and the sister species of melon is from Australia. Proc Natl Acad Sci USA, 2010,107(32):14269-14273.
[90]	Chen JF, Staub JE, Jiang JM . A reevaluation of karyotype in cucumber (Cucumis sativus L.). Genet Resour Crop Ev, 1998,45(4):301-305.
[91]	Qian CT, Chen JF, Luo XD, Cao QH, Lei C . C-banding of prometaphase chromosomes in Cucumber and the procedure for slide preparation. Acta Hortic Sin, 2004,31(6):814-816.
	钱春桃, 陈劲枫, 罗向东, 曹清河, 雷春 . 黄瓜前中期染色体C-分带及其制备方法研究. 园艺学报, 2004,31(6):814-816.
[92]	Zhang YX, Cheng CY, Li J, Yang SQ, Wang YZ, Li ZA, Chen JF, Lou QF . Chromosomal structures and repetitive sequences divergence in Cucumis species revealed by comparative cytogenetic mapping. BMC Genomics, 2015,16(1):730.
[93]	Wang YZ, Zhang ZT, Jia L, Li ZA, Li J, Lou QF, Chen JF . Molecular and cytogenetic analyses provide evidence of the introgression of chromosomal segments from the wild Cucumis hystrix into the cultivated cucumber through the bridge of a synthetic allotetraploid. Mol Breeding, 2017,37(7):89.
[94]	Chen JF, Stau JE, Adelberg JW, Jiang JM . Physical mapping of 45S rRNA genes in Cucumis species by fluorescence in situ hybridization. Can J Bot, 1999,77:389-393.
[95]	Zhang ZT, Yang SQ, Li ZA, Zhang YX, Wang YZ, Cheng CY, Li J, Chen JF, Lou QF . Comparative chromosomal localization of 45S and 5S rDNAs and implications for genome evolution in Cucumis. Genome, 2016,59(7):449-457.
[96]	Lou QF, Zhang YX, He YH, Li J, Jia L, Cheng CY, Guan W, Yang SQ, Chen JF . Single-copy gene-based chromosome painting in cucumber and its application for chromosome rearrangement analysis in Cucumis. Plant J, 2014,78(1):169-179.
[97]	Li ZA, Bi YF, Wang X, Wang YZ, Yang SQ, Zhang ZT, Chen JF, Lou QF . Chromosome identification in Cucumis anguria revealed by cross-species single-copy gene FISH. Genome, 2018,61(6):397-404.
[98]	Bi YF, Zhao QZ, Yan WK, Li MX, Liu YX, Cheng CY, Lu Z, Yu XQ, Li J, Qian CT, Wu YF, Chen JF, Lou QF . Flexible chromosome painting based on multiplex PCR of oligonucleotides and its application for comparative chromosome analyses in Cucumis. Plant J, 2020,102(1):178-186.
[99]	Zhao QZ, Wang YZ, Bi YF, Zhai YF, Yu XQ, Cheng CY, Wang PQ, Li J, Lou QF, Chen JF . Oligo-painting and GISH reveal meiotic chromosome biases and increased meiotic stability in synthetic allotetraploid Cucumis ×hytivus with dysploid parental karyotypes. BMC Plant Biol, 2019,19(1):471.
[100]	Yang SQ, Cheng CY, Qin XD, Yu XQ, Lou QF, Li J, Qian CT, Chen JF . Comparative cyto-molecular analysis of repetitive DNA provides insights into the differential genome structure and evolution of five Cucumis species. Hortic Plant J, 2019,5(5):192-204.
[101]	Han YH, Zhang ZH, Liu CX, Liu JH, Huang SW, Jiang JM, Jin WW . Centromere repositioning in cucurbit species: implication of the genomic impact from centromere activation and inactivation. Proc Natl Acad Sci USA, 2009,106(35):14937-14941.
[102]	Yang LM, Koo DH, Li YH, Zhang XJ, Luan FS, Havey MJ, Jiang JM, Weng YQ . Chromosome rearrangements during domestication of cucumber as revealed by high- density genetic mapping and draft genome assembly. Plant J, 2012,71(6):895-906.
[103]	Cao QH, Chen JF, Qian CT . Identification and characterization of a Cucumber alien translocation lineCT201 possessing resistance to downy mildew. Acta Hortic Sin, 2005,32(6):1098-1101.
	曹清河, 陈劲枫, 钱春桃 . 黄瓜抗霜霉病异源易位系CT201的筛选与鉴定. 园艺学报, 2005,32(6):1098-1101.
[104]	Chen JF, Lin MS, Qian CT, Zhuang FY, Stephen L . Identification of Meloidogyne incognita (kofoid & white) chitwood resistance in C ucumis hystrix Chakr. and the progenies of its interspecific hybrid with cucumber( C. sativus L.). J Nanjing Agric Univ, 2001,1:21-24.
	陈劲枫, 林茂松, 钱春桃, 庄飞云 , Stephen Lewis. 甜瓜属野生种及其与黄瓜种间杂交后代抗根结线虫初步研究. 南京农业大学学报, 2001,1:21-24.
[105]	Chen JF, Adelberg J . Interspecific hybridization in Cucumis-progress, problems, and perspectives. Hort Sci, 2000,35(1):11-15.
[106]	Chen J, Staub J, Qian C, Jiang J, Luo X, Zhuang F . Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr. × Cucumis sativus L. Theor Appl Genet, 2003,106(4):688-695.
[107]	Chen JF, Luo XD, Staub JE, Jahn MM, Qian CT, Zhuang FY, Ren G . An allotriploid derived from a amphidiploid × diploid. Euphytica, 2003,131(2):235-241.
[108]	Chen JF, Luo XD, Qian CT, Jahn MM, Staub JE, Zhuang FY, Lou QF, Ren G . Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses. Theor Appl Genet, 2004,108(7):1343-1348.
[109]	Li MX, Zhao QZ, Liu YX, Qin XD, Hu W, Davoudi M, Chen JF, Lou QF . Development of alien addition lines from Cucumis hystrix in Cucumis sativus: cytological and molecular marker analyses. Genome, 2020,63(12):629-641.
[110]	Guan B, Wang K, Zhou BL, Guo WZ, Zhang TZ . Establishment of a multi-color genomic in situ hybridization technique to simultaneously discriminate the three interspecific hybrid genomes in Gossypium. J Integr Plant Biol, 2008,50(3):345-351.
[111]	Wang K, Guo WZ, Zhang TZ . Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L. Theor Appl Genet, 2007,115(5):675-682.
[112]	Wang K, Guan B, Guo WZ, Zhou BL, Hu Y, Zhu YC, Zhang TZ . Completely distinguishing individual A-genome chromosomes and their karyotyping analysis by multiple bacterial artificial chromosome-fluorescence in situ hybridization. Genetics, 2008,178(2):1117-1122.
[113]	Wang K, Song XL, Han ZG, Guo WZ, Yu JZ, Sun J, Pan JJ, Kohel RJ, Zhang TZ . Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theor Appl Genet, 2006,113(1):73-80.
[114]	Wang KB, Wang ZW, Li FG, Ye WW, Wang JY, Song GL, Yue Z, Cong L, Shang HH, Zhu SL, Zou CS, Li Q, Yuan YL, Lu CR, Wei HL, Gou CY, Zheng ZQ, Yin Y, Zhang XY, Liu K, Wang B, Song C, Shi N, Kohel RJ, Percy RG, Yu JZ, Zhu YX, Wang J, Yu SX . The draft genome of a diploid cotton Gossypium raimondii. Nat Genet, 2012,44(10):1098-1103.
[115]	Paterson AH, Wendel JF, Gundlach H, Guo H, Jenkins J, Jin DC, Llewellyn D, Showmaker KC, Shu SQ, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu GJ, Lee TH, Li JP, Lin LF, Liu T, Marler BS, Page JT, Roberts AW, Romanel E, Sanders WS, Szadkowski E, Tan X, Tang HB, Xu CM, Wang JP, Wang ZN, Zhang D, Zhang L, Ashrafi H, Bedon F, Bowers JE, Brubaker CL, Chee PW, Das S, Gingle AR, Haigler CH, Harker D, Hoffmann LV, Hovav R, Jones DC, Lemke C, Mansoor S, ur Rahman M , Rainville LN, Rambani Ai, Reddy UK, Rong JK, Saranga Y, Scheffler BE, Scheffler JA, Stelly DM, Triplett BA, Van Deynze A, Vaslin MFS, Waghmare VN, Walford SA, Wright RJ, Zaki EA, Zhang TZ, Dennis ES, Mayer KFX, Peterson DG, Rokhsar DS, Wang XY, Schmutz J. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature, 2012,492(7429):423-427.
[116]	Li FG, Fan GY, Wang KB, Sun FM, Yuan YL, Song GL, Li Q, Ma ZY, Lu CR, Zou CS, Chen WB, Liang XM, Shang HH, Liu WQ, Shi CC, Xiao GH, Gou CY, Ye WW, Xu X, Zhang XY, Wei HL, Li ZF, Zhang GY, Wang JY, Liu K, Kohel RJ, Percy RG, Yu JZ, Zhu YX, Yu SX . Genome sequence of the cultivated cotton Gossypium arboreum. Nat Genet, 2014,46(6):567-572.
[117]	Zhang TZ, Hu Y, Jiang WK, Fang L, Guan XY, Chen JD, Zhang JB, Saski CA, Scheffler BE, Stelly DM, Hulse-Kemp AM, Wan Q, Liu BL, Liu CX, Wang S, Pan MQ, Wang YK, Wang DW, Ye WX, Chang LJ, Zhang WP, Song QX, Kirkbride RC, Chen XY, Dennis E, Llewellyn DJ, Peterson DG, Thaxton P, Jones DC, Wang Q, Xu XY, Zhang H, Wu HT, Zhou L, Mei GF, Chen SQ, Tian Y, Xiang D, Li XH, Ding J, Zuo QY, Tao LN, Liu YC, Li J, Lin Y, Hui YY, Cao ZS, Cai CP, Zhu XF, Jiang Z, Zhou BL, Guo WZ, Li RQ, Chen ZJ . Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement. Nat Biotech, 2015,33(5):531-537.
[118]	Li FG, Fan GY, Lu CR, Xiao GH, Zou CS, Kohel RJ, Ma ZY, Shang HH, Ma XF, Wu JY, Liang XM, Huang G, Percy RG, Liu K, Yang WH, Chen WB, Du XG, Shi CC, Yuan YL, Ye WW, Liu X, Zhang XY, Liu WQ, Wei HL, Wei SJ, Huang GD, Zhang XL, Zhu SJ, Zhang H, Sun FM, Wang XF, Liang J, Wang JH, He Q, Huang LH, Wang J, Cui JJ, Song GL, Wang KB, Xu X, Yu JZ, Zhu YX, Yu SX . Genome sequence of cultivated upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution. Nat Biotech, 2015,33(5):524-530.
[119]	Cao YJ . Studies of centromere-specific sequences in cotton[Dissertation]. Nanjing Agricultural University, 2013.
	曹玉洁 . 棉花着丝粒区特异序列的发掘与分析[学位论文]. 南京农业大学, 2013.
[120]	Zhang WP, Cao YJ, Wang K, Zhao T, Chen JD, Pan MQ, Wang Q, Feng SL, Guo WZ, Zhou BL, Zhang TZ . Identification of centromeric regions on the linkage map of cotton using centromere-related repeats. Genomics, 2014,104(6 Pt B):587-593.
[121]	Zhang ZX . The isolation of telomeric DNA sequences G. arboreum and G. raimondii[Dissertation]. Nanjing Agricultural University, 2013.
	张忠鑫 . 亚洲棉及雷蒙德氏棉端粒DNA序列的发掘与分析[学位论文]. 南京农业大学, 2013.
[122]	Chen Y, Wang YY, Zhao T, Yang JW, Feng SL, Nazeer W, Zhang TZ, Zhou BL . A new synthetic amphiploid (AADDAA) between Gossypium hirsutum and G. arboreum lays the foundation for transferring resistances to verticillium and drought. PLoS One, 2015,10(6):e0128981.
[123]	Chen Yu, Wang YY, Wang K, Zhu XF, Guo WZ, Zhang TZ, Zhou BL . Construction of a complete set of alien chromosome addition lines from Gossypium australe in Gossypium hirsutum: morphological, cytological, and genotypic characterization. Theor Appl Genet, 2014,127(5):1105-1121.
[124]	Wang YY, Feng SL, Li S, Tang D, Chen Y, Chen Y, Zhou BL . Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum. BMC Genomics, 2018,19(1):15.
[125]	Wang XX, Wang YY, Wang C, Chen Y, Chen Y, Feng SL, Zhao T, Zhou BL . Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers. BMC Plant Biol, 2016,16(1):218.
[126]	Wang C . Development of alien addition lines of Gossypium hirsutum-Gossypium anomalum and evaluation of its excellent characters[Dissertation]. Nanjing Agricultural University, 2018.
	王琛 . 陆地棉-异常棉异附加系创制与优异性状鉴定 [学位论文]. 南京农业大学, 2018.
[127]	Tang D, Feng SL, Li S, Chen Y, Zhou BL . Ten alien chromosome additions of Gossypium hirsutum-Gossypium bickii developed by integrative uses of GISH and species-specific SSR markers. Mol Genet Genomics, 2018,293(4):945-955.
[128]	Tanaka R . On the speciation and karyotypes in diploid and tetraploid species of Chrysanthemum. Cytologia, 1960,25(1):43-58.
[129]	Fedorov AV, Fedorov AA, Fedorov YA . Chromosome numbers of flowering plants. West Genabt: Otto Koeltz Science Publishers, 1974.
[130]	Li MX, Zhang XF, Chen JY . Cytological studies on some Chinese wild dendranthema species and chrysanthemum cultivars. Acta Hortic Sin, 1983, ( 3):199-206.
	李懋学, 张斅方, 陈俊愉 . 我国某些野生和栽培菊花的细胞学研究. 园艺学报, 1983, ( 3):199-206.
[131]	Du BQ, Liu QH, Zhu CY, Ke SQ . Karyotype studies of two species on dendranthema. J Wuhan Bot Res, 1989, ( 3):293-296.
	杜冰群, 刘启宏, 朱翠英, 柯善强 . 两种菊属植物的核型研究. 武汉植物学研究, 1989, ( 3):293-296.
[132]	Wang JW, Yang J, Li MX . Karyotype study of five species of chinese dendranthema. Acta Bot Yunnanica, 1991, ( 4):411-416.
	汪劲武, 杨继, 李懋学 . 国产五种菊属植物的核型研究. 云南植物研究, 1991, ( 4):411-416.
[133]	Wang JW, Yang J, Li MX . The morphological variation and the karyotypical characters of Dendranthema indicum and D. lavandulifolium. Acta Phytotaxon Sin, 1993,31(2):140-146.
	汪劲武, 杨继, 李懋学 . 野菊和甘菊的形态变异及其核型特征. 植物分类学报, 1993,31(2):140-146.
[134]	Endo N . The chromosome survey on the cultivated chrysanthemums, Chrysanthemum morifolium Ram. J Jpn Soc Hortic Sci, 1969,38(3):267-274.
[135]	Endo M . On the occurrence of B chromosome in the garden chrysanthemum, Chrysanthemum morifolium Ramat. J Jpn Soc Hortic Sci, 1990,59(3):613-620.
[136]	Chen FD, Zhao HB, Li C, Chen SM, Fang WM . Advances in cytology and molecular cytogenetics of the genus Dendranthema. J Nanjing Agric Univ, 2008,31(1):118-126.
	陈发棣, 赵宏波, 李畅, 陈素梅, 房伟民 . 菊属植物细胞学与分子细胞遗传学研究进展. 南京农业大学学报, 2008,31(1):118-126.
[137]	Kim JS, Pak JH, Seo BB, Tobe H . Karyotypes of metaphase chromosomes in diploid populations of Dendranthema zawadskii and related species ( Asteraceae) from Korea: diversity and evolutionary implications. J Plant Res, 2003,116(1):47-54.
[138]	Li C, Chen FD, Zhao HB, Chen SM . Karyotype diversity of 17 chrysanthemum cultivars with small inflorescences. Acta Hortic Sin, 2008,35(1):71-80.
	李畅, 陈发棣, 赵宏波, 陈素梅 . 栽培小菊17个品种的核型多样性. 园艺学报, 2008,35(1):71-80.
[139]	Tang FP, Chen FD, Chen SM, Teng NJ, Fang WM . Intergeneric hybridization and relationship of genera within the tribe Anthemideae Cass. (I. Dendranthema crassum(Kitam.) Kitam. × Crossostephium chinense, 2009,169:133-140.
[140]	Deng YM, Chen SM, Lu AM, Chen FD, Tang FP, Guan ZY, Teng NJ . Production and characterisation of the intergeneric hybrids between Dendranthema morifolium and Artemisia vulgaris exhibiting enhanced resistance to chrysanthemum aphid (Macrosiphoniella sanbourni). Planta, 2010,231(3):693-703.
[141]	Qi XY, Zhang F, Guan ZY, Wang HB, Jiang JF, Chen SM, Chen FD . Localization of 45S and 5S rDNA sites and karyotype of Chrysanthemum and its related genera by fluorescent in situ hybridization. Biochem Syst Ecol, 2015,62:164-172.
[142]	Chen JY . Studies on the origin of Chinese florist’s chrysanthemum. Acta Hortic, 1985,167:349-361.
[143]	Dai SL, Wang WK, Huang JP . Advances of researches on phylogeny of Dendranthema and origin of chrysanthemum. J Beijing Fore Univ, 2002,24(5/6):230-234.
	戴思兰, 王文奎, 黄家平 . 菊属系统学及菊花起源的研究进展. 北京林业大学学报, 2002,24(5/6):230-234.
[144]	Chen FD, Chen PD, Li HJ . Genome analysis and their phylogenetic relationships of several wild species of Dendranthema in China. Acta Hortic Sin, 1996,23(1):67-72.
	陈发棣, 陈佩度, 李鸿渐 . 几种中国野生菊的染色体组分析及亲缘关系初步研究. 园艺学报, 1996,23(1):67-72.
[145]	Chen FD, Chen PD, Fang WM, Li HJ . Cytological studies of three varieties of medicinal chrysanthemum. J Nanjing Agric Univ, 1997,20(2):28-31.
	陈发棣, 陈佩度, 房伟民, 李鸿渐 . 3个药用菊品种的细胞学研究. 南京农业大学学报, 1997,20(2):28-31.
[146]	Chen FD, Chen XL, Fang WM . On cytology of three geocotypes of Dendranthema Indicum. Adv Horticult(Ⅱ), 1998, 693-696.
	陈发棣, 陈秀兰, 房伟民 . 3个地理居群野菊的细胞学研究. 园艺学进展(第2辑), 1998, 693-696.
[147]	Li XL, Chen FD . RAPD analysis of wild species, cultivars and interspecific hybrids in Dendranthema. J Nanjing Agric Univ, 2004,27(3):29-33.
	李辛雷, 陈发棣 . 菊属野生种、栽培菊花及种间杂种的RAPD分析. 南京农业大学学报, 2004,27(3):29-33.
[148]	Cui NX . Studies on meiosis behaviors and genetic relationships of several Dendranthemum species and their hybrids[Dissertation]. Nanjing Agricultural University, 2004.
	崔娜欣 . 几种菊属植物及其杂种减数分裂行为与亲缘关系研究[学位论文]. 南京农业大学, 2004.
[149]	Chen JY. The origin of garden chrysanthemum. Hefei: Anhui Science and Technology Press. 2012, 67-74.
	陈俊愉. 菊花起源. 合肥:安徽科学技术出版社. 2012, 67-74.
[150]	Song C, Liu YF, Song AP, Dong, GQ, Zhao HB, Sun W, Ramakrishnan S, Wang Y, Wang SB, Li TZ, Niu Y, Jiang JF, Dong B, Xia Y, Chen SM, Hu ZG, Chen FD, Chen SL . The Chrysanthemum nankingense genome provides insights into the evolution and diversification of chrysanthemum flowers and medicinal traits. Mol Plant, 2018,11(12):1482-1491.
[151]	Hirakawa H, Sumitomo K, Hisamatsu T, Nagano S, Shirasawa K, Higuchi Y, Kusaba M, Koshioka M, Nakano Y, Yagi M, Yamaguchi H, Taniguchi K, Nakano M, Isobe SN . De novo whole-genome assembly in Chrysanthemum seticuspe, a model species of Chrysanthemums, and its application to genetic and gene discovery analysis. DNA Res, 2019,26(3):195-203.
[152]	Lan W, Chen SM, Yin DM, Chen FD . Studies on in vitro conservation of Chrysanthemum yoshinaganthum. Acta Hortic Sin, 2010,37(12):2007-2016.
	兰伟, 陈素梅, 尹冬梅, 陈发棣 . 那贺川野菊的离体保存. 园艺学报, 2010,37(12):2007-2016.
[153]	Xu Y, Chen FD . The LT50 and cold tolerance adaptability of Chrysanthemum during a natural drop in temperature. Acta Hortic Sin, 2008,35(4):559-564.
	许瑛, 陈发棣 . 菊花8个品种的低温半致死温度及其抗寒适应性. 园艺学报, 2008,35(4):559-564.
[154]	Li N, Fang WM, Chen FD, Chen SM, Chen Y . Physiological indexes in florets of two winter cut chrysanthemum cultivars under low temperature and their cold tolerance. Acta Bot Boreali-Occident Sin, 2010,30(4):741-746.
	李娜, 房伟民, 陈发棣, 陈素梅, 陈煜 . 切花寒菊小花对低温胁迫的生理响应及其抗寒性分析. 西北植物学报, 2010,30(4):741-746.
[155]	He JP, Chen FD, Chen SM, Fang WM . Aphid-resistance of chrysanthemum cultivars. Chin J Ecol, 2010,29(7):1382-1386.
	何俊平, 陈发棣, 陈素梅, 房伟民 . 不同菊花品种抗蚜虫性鉴定. 生态学杂志, 2010,29(7):1382-1386.
[156]	Sun Y . Studies on aphid resistance and mechanisms in chrysanthemum and it’s related species at seedling stage [Dissertation]. Nanjing Agricultural University, 2011.
	孙娅 . 菊花近缘种属植物苗期抗蚜虫性鉴定与抗蚜机理研究[学位论文]. 南京农业大学, 2011.
[157]	Zhang FJ, Wang ZQ, Dong W, Sun CQ, Wang HB, Song AP, He LZ, Fang WM, Chen FD, Teng NJ . Transcriptomic and proteomic analysis reveals mechanisms of embryo abortion during chrysanthemum cross breeding. Sci Rep, 2014,4:6536.
[158]	Deng YM, Chen SM, Chen FD, Cheng X, Zhang F . The embryo rescue derived intergeneric hybrid between chrysanthemum and Ajania przewalskii shows enhanced cold tolerance. Plant Cell Rep, 2011,30(12):2177-2186.
[159]	Cheng X, Chen SM, Chen FD, Fang WM, Deng YM, She LF . Interspecific hybrids between Dendranthema morifolium(Ramat.) Kitamura and D. nankingense , 2010,172:101-108.
[160]	Deng YM, Jiang JF, Chen SM, Huang CB, Fang WM, Chen FD . Drought tolerance of intergeneric hybrids between Chrysanthemum morifolium and Ajania przewalskii. Sci Hortic, 2012,148(4):17-22.
[161]	Sun CQ, Chen FD, Teng NJ, Liu ZL, Fang WM, Hou XL . Interspecific hybrids between Chrysanthemum grandiflorum(Ramat.) Kitamura and C. indicum, 2010,174:51-60.
[162]	Tang FP, Wang HB, Chen SM, Chen FD, Teng NJ, Liu ZL . First intergeneric hybrids within the tribe Anthemideae Cass.III. Chrysanthemum indicum L. Des Moul. × Opisthopappus taihangensis( Ling) Shih. Biochem Syst Ecol, 2012,43:87-92.
[163]	Zhu WY, Jiang JF, Chen SM, Wang L, Xu LL, Wang HB, Li PL, Guan ZY, Chen FD . Intergeneric hybrid between Chrysanthemum × morifolium and Artemisia japonica achieved via embryo rescue shows salt tolerance. Euphytica, 2013,191:109-119.
[164]	Zhu WY, Zhang F, Chen SM, Xu LL, Wang L, Wang HB, Qi XY, Li HY, Chen FD . Intergeneric hybrids between Chrysanthemum morifolium ‘Nannongxiaoli’ and Artemisia vulgaris ‘Variegata’ show enhanced resistance against both aphids and alternaria leaf spot. Euphytica, 2014,197:399-408.
[165]	Chen FD, Chen SM, Fang WM, Zhang F, Jiang JF, Teng NJ, Guan ZY, Wang HB, Song AP, Zhao S . Discovery of excellent chrysanthemum germplasms and germplasm enhancement. Bull Natl Nat Sci Found Chin, 2016,30(2):112-115.
	陈发棣, 陈素梅, 房伟民, 张飞, 蒋甲福, 滕年军, 管志勇, 王海滨, 宋爱萍, 赵爽 . 菊花优异种质资源挖掘与种质创新研究. 中国科学基金, 2016,30(2):112-115.
[166]	Bradshaw HD, Ceulemans R, Davis J, Stettler R . Emerging model systems in plant biology: poplar (Populus) as a model forest tree. J Plant Growth Regul, 2000,19(3):306-313.
[167]	Zhang Y, Zhang SG, Qi LW, Chen XQ, Chen RY, Song WQ . Poplar as a model for forest tree in genome research. Chin Bull Bot, 2006,23(3):286-293.
	张勇, 张守攻, 齐力旺, 陈小强, 陈瑞阳, 宋文芹 . 杨树——林木基因组学研究的模式物种. 植物学报, 2006,23(3):286-293.
[168]	Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, Déjardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasjärvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Leplé JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouzé P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G , Van de Peer Y, Rokhsar D. The genome of black cottonwood, Populus trichocarpa( Torr. & Gray). Science, 2006,313(5793):1596-1604.
[169]	Yin TM . A review of the genomic and gene cloning studies in trees. Hereditas(Beijing), 2010,32(7):677-684.
	尹佟明 . 林木基因组及功能基因克隆研究概述. 遗传, 2010,32(7):677-684.
[170]	Ma T, Wang JY, Zhou GK, Yue Z, Hu QJ, Chen Y, Liu BB, Qiu Q, Wang Z, Zhang J, Wang K, Jiang DC, Gou CY, Yu LL, Zhan DL, Zhou R, Luo WC, Ma H, Yang YZ, Pan SK, Fang DM, Luo YD, Wang X, Wang GN , Wang, J, Wang Q, Lu X, Chen Z, Liu JC, Lu Y, Yin Y, Yang HM, Abbott RJ, Wu YX, Wan DS, Li J, Yin TM, Lascoux M, Difazio SP, Tuskan GA, Wang J, Liu JQ. Genomic insights into salt adaptation in a desert poplar. Nat Commun, 2013,4:2797.
[171]	Ma JC, Wan DS, Duan BB, Bai XT, Bai QX, Chen NN, Ma T . Genome sequence and genetic transformation of a widely distributed and cultivated poplar. Plant Biotechnol J, 2019,17(2):451-460.
[172]	Ribeiro T, Barão A, Viegas W, Morais-Cecíli L . Molecular cytogenetics of forest trees. Cytogenet Genome Res, 2008,120(3-4):220-227.
[173]	Islam-Faridi MN, Nelson CD, Difazio SP, Gunter LE, Tuskan GA . Cytogenetic analysis of Populus trichocarpa--ribosomal DNA, telomere repeat sequence, and marker-selected BACs. Cytogenet Genome Res, 2009,125(1):74-80.
[174]	Blackburn KB, Harrison JWH . A preliminary account of the chromosomes and chromosome behaviour in the Salicaceae. Ann Bot, 1924,38(2):361-378.
[175]	Einspahr DW, Van Buijtenen JP, Peckham JR . Natural variation and heritability in triploid aspe. Silvae Genet, 1963,12(2):51-58.
[176]	Kang XY, Zhu ZT, Zhang ZY . Cytogenetic studies on the origin of chinese white poplar. J Beijing Fore Univ, 1999,21(6):6-10.
	康向阳, 朱之悌, 张志毅 . 毛白杨起源的细胞遗传学研究. 北京林业大学学报, 1999,21(6):6-10.
[177]	Xin HY, Wang S, Liu GX, Zhen Y, Shi JS, Kuang HL, Xi ML . Relationship between the meiosis processes of microsporocytes and morphology of male flower buds and anthers in Populus deltoides Marsh. J Nanjing Forest Univ (Nat Sci Ed), 2016, ( 2):48-52.
	辛昊阳, 王帅, 刘光欣, 甄艳, 施季森, 匡华琳, 席梦利 . 美洲黑杨小孢子母细胞减数分裂进程与花芽及花药外观形态相关性研究. 南京林业大学学报(自然科学版), 2016, ( 2):48-52.
[178]	Lan Y, Xin HY, Zhao YL, Sun ZQ, Dai X, Xie ZW, Xi ML . Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr. J Nanjing Forest Univ(Nat Sci Ed), 2017, ( 6):177-180.
	兰月, 辛昊阳, 赵乙琏, 孙志强, 戴筱, 解志伟, 席梦利 . 小叶杨花粉母细胞减数分裂及花粉萌发力观测. 南京林业大学学报(自然科学版), 2017, ( 6):177-180.
[179]	Dong FP, Han SY, Zhang SG, Qi LW, Liu B, Li XL, Chen CB . Physical mapping of 25S rDNA on metaphase chromosomes of Populus(Salicaceae) in five sections by fluorescence in situ Hybridization. Acta Bot Yunnanica, 2007,29(4):423-428.
	董凤平, 韩素英, 张守攻, 齐力旺, 刘博, 李秀兰, 陈成彬 . 25S rDNA在杨属植物染色体上的定位. 云南植物研究, 2007,29(4):423-428.
[180]	Hu BQ, Dong FP, Wang CG, Qi LW, Song WQ, Chen CB . Multicolor fluorescence in situ Hybridization of seven Populus species-ribosomal DNA and telomere repeat sequence. Acta Sci Natl Univ Nankai(Nat Sci Ed), 2012,45(1):58-64.
	胡宝全, 董凤平, 王春国, 齐力旺, 宋文芹, 陈成彬 . 五派杨树多色荧光原位杂交分析(英文). 南开大学学报(自然科学版), 2012,45(1):58-64.
[181]	Xin HY, Zhang T, Wu YF, Zhang WL, Zhang PD, Xi ML, Jiang JM . An extraordinarily stable karyotype of the woody Populus species revealed by chromosome painting. Plant J, 2020,101(2):253-264.
[182]	Xin HY, Zhang T, Han YH, Wu YF, Shi JS, Xi ML, Jiang JM . Chromosome painting and comparative physical mapping of the sex chromosomes in Populus tomentosa and Populus deltoides. Chromosoma, 2018,127(3):313-321.
[183]	Nakajima G . Chromosome numbers in some crops and wild angiosperms. Jpn J Genet, 1931,12:211-217.
[184]	Liang GL, Xiang SQ, Wang WX, Yan Y, Li XL, Pei Y, Zhang DP . Karyotype analysis of the wild Ipomoea trifida(2x, 6x) complex closely related to the sweet potato. Adv Chromosome Sci, 2001,1:404-406.
	梁国鲁, 向素琼, 汪卫星, 闫勇, 李晓林, 裴炎, 张大鹏 . 甘薯近缘野生种Ipomoea trifida (2X、6X)的核型分析. Advances in Chromosome Sciences, 2001,1:404-406.
[185]	Cao QH, Ma DF, Zhang A . Chromosome karyotype and pollen ultrastructure of a related species of sweet potato. Acta Bot Boreali-Occident Sin, 2008,28(8):1610-1613.
	曹清河, 马代夫, 张安 . 甘薯近缘种染色体核型及花粉粒超微结构分析. 西北植物学报, 2008,28(8):1610-1613.
[186]	Tang JL, Qi DS, Zhang Y, Liu HJ, Sun JY, Cao QH, Ma DF, Li ZY . FISH analysis of chromosomes of sweet potato (Ipomoea batatas cv. Xushu No.18). Hereditas (Beijing), 2010,32(2):177-182.
	汤佳立, 戚大石, 张俞, 刘慧娟, 孙健英, 曹清河, 马代夫, 李宗芸 . 荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv. Xushuno.18)染色体. 遗传, 2010,32(2):177-182.
[187]	An TT, Tang JL, Sun JY, Cao QH, Ma DF , Li ZY. rDNA-FISH analysis and DAPI-karyotype of Ipomoea batatas cv.and Ipomoea hederacea Jacq. Acta Bot Boreali-Occident Sin, 2012,32(4):682-687.
	安婷婷, 汤佳立, 孙健英, 曹清河, 马代夫, 李宗芸 . 甘薯栽培种及其近缘野生种的DAPI核型及rDNA- FISH分析. 西北植物学报, 2012,32(4):682-687.
[188]	Xiang SQ, Wang WX, Li XL, Chen Y, Guo QG, He Q, Liang GL . GISH analysis of sweet potato wild relative Ipomoea trifida(4x). Acta Agron Sin, 2008,34(2):341-343.
	向素琼, 汪卫星, 李晓林, 陈瑶, 郭启高, 何桥, 梁国鲁 . 甘薯近缘野生种Ipomoea trifida (4x) GISH分析. 作物学报, 2008,34(2):341-343.
[189]	Sun JY, Yu LX, Cai ZX, Zhang A, Jin WW, Han YH, Li ZY . Comparative karyotype analysis among six Ipomoea species based on two newly identified repetitive sequences. Genome, 2019,62(4):243-252.
[190]	Shen JQ. A simple technique for the determination of cross incompatibility groups in sweet potato varieties. Bull Agric Sci Technol, 1984, 8: 12-13+2.
	沈稼青 . 甘薯品种杂交不亲和群的简易测定技术, 农业科技通讯, 1984, 8: 12-13+2.
[191]	Li ZY, Chen FY, Jiang JJ, Yu LX, Sun JY, Han YH . Cytogenetics research progress of sweet potato. J South- Central Univ for Natl(Nat Sci Ed), 2016,35(4):34-39.
	李宗芸, 陈孚尧, 蒋姣姣, 俞立璇, 孙健英, 韩永华 . 甘薯细胞遗传学研究进展. 中南民族大学学报(自然科学版), 2016,35(4):34-39.
[192]	Cao QH, Zhang A, Ma DF, Li HM, Li Q, Li P . Novel interspecific hybridization between sweetpotato (Ipomoea batatas(L.) Lam.) and its two diploid wild relatives. Euphytica, 2009,169(3):345-352.
[193]	Cao QH, Tang J, Li A, Gruneberg W, Huamani K, Ma DF . Ploidy Level and Molecular Phylogenic Relationship among Novel Ipomoea Interspecific Hybrids. Czech J Genet Plant Breed, 2014,50(1):32-38.
[194]	do Vale Martins L, Yu Fan, Zhao HN, Dennison T, Lauter N, Wang HY, Deng ZH, Thompson A, Semrau K, Rouillard JM, Birchler JA, Jiang JM . Meiotic crossovers characterized by haplotype specific chromosome painting in maize. Nat Commun, 2019,10(1):4064.
[195]	Koo DH, Molin WT, Saski CA, Jiang JM, Putta K, Jugulam M, Friebe M, Gill BS . Extrachromosomal circular DNA-based amplification and transmission of herbicide resistance in crop weed Amaranthus palmeri. Proc Natl Acad Sci USA, 2018,115(13):3332-3337.
[196]	Eng CHL, Lawson M, Zhu Q, Dries R, Koulena N, Takei Y, Yun J, Cronin C, Karp C, Yuan GC, Cai L . Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH. Nature, 2019,568(7751):235-239.
[197]	Battich N, Stoeger T, Pelkmans L . Image-based transcriptomics in thousands of single human cells at single-molecule resolution. Nat Methods, 2013,10(11):1127-1133.
[198]	Lubeck E, Cai L . Single-cell systems biology by super-resolution imaging and combinatorial labeling. Nat Methods, 2012,9(7):743-748.
[199]	Feng C, Yuan J, Bai H, Liu YL, Su HD, Liu Y, Shi LD, Gao Z, Birchler JA, Han FP . The deposition of CENH3 in maize is stringently regulated. Plant J, 2020,102(1):6-17.

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名称	探针分子类型	探针标记物	杂交对象	研究目的	参考文献
GISH 基因组原位杂交	总基因组DNA	荧光素	有丝分裂中期染色体减数分裂粗线期染色体减数分裂中期I染色体	外源染色质鉴定染色体结构重排	[24,26,29]
FISH 荧光原位杂交	(1)重复序列DNA (2) BAC克隆DNA (BAC-FISH) (3)质粒DNA (4)单拷贝基因DNA 序列(single gene-FISH) (5)寡核苷酸DNA (oligo-FISH) (6)单基因寡核苷酸探针库(unigene oligo-painting)	荧光素/ 半抗原	有丝分裂中期染色体减数分裂粗线期染色体减数分裂中期I染色体间期核DNA纤维	外源染色质鉴定染色体上序列实际位置和顺序确定物种间进化关系染色体结构重排物种核型构建染色体构型分析转基因鉴定基因组拼接质量评价	[39,59,60,68~70,98,99,109,181, 182,193~195]
seqFISH RNA-seq荧光原位杂交 smFISH 单分子荧光原位杂交	RNA	荧光素	特定组织中的单个细胞	转录水平RNA位置和数量变化	[196~198]
Immuno -staining 蛋白免疫共定位	蛋白抗体	荧光素	有丝分裂和减数分裂的细胞或者特定组织中的细胞	翻译水平蛋白质的位置和数量变化,表观基因组修饰	[199]

Research progress of plant cytogenetics in Jiangsu province

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