Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (4): 375-384.doi: 10.16288/j.yczz.21-005
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MDMPR: Mouse Developmental and Metabolic Phenotype Repository
Wenjing Zhu, Zhiwei Liu(
)
- Cambridge-Suda Genomic Resource Center, Soochow University, Suzhou 215123, China
-
Received:2021-01-05Revised:2021-03-04Online:2021-04-20Published:2021-04-20 -
Contact:Liu Zhiwei E-mail:liuzhiwei@cam-su.org -
Supported by:Supported by the National Key R&D Program of the Ministry of Science and Technology of China No(2018YFA0801100)
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Wenjing Zhu, Zhiwei Liu. MDMPR: Mouse Developmental and Metabolic Phenotype Repository[J]. Hereditas(Beijing), 2021, 43(4): 375-384.
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Fig. 1
Knockout first targeting strategy based on mutant mouse ES cells"
Fig. 1
Fig. 2
CRISPR/Cas9 Knockout strategy"
Fig. 2
Table 1
Summary of genetic background and microbial quality control"
| 质控 | 单位 | 检测方 | 检测频次 | 总检测次数 | 检测结果 |
|---|---|---|---|---|---|
| 遗传背景质控 | CAM-SU GRC | 苏州西山生物有限公司 | 半年 | 2 | 合格 |
| MARC | 江苏集萃药康生物科技有限公司 | 半年 | 2 | 合格 | |
| 微生物质控 | CAM-SU GRC | 苏州西山生物有限公司 | 隔月 | 8 | 合格 |
| MARC | 江苏集萃药康生物科技有限公司 | 季度 | 4 | 合格 |
Table 1
Fig. 3
Standardized phenotyping pipeline of development and metabolism"
Fig. 3
Fig. 4
Structure of experimental parameter"
Fig. 4
Fig. 5
MDMPR system structure"
Fig. 5
Fig. 6
Interface design of MDMPR"
Fig. 6
Table 1
Microbiological quality control standards of CAM-SU GRC"
| 病原体 | 检测项目 | 英文名称 | 质控标准 |
|---|---|---|---|
| 病毒 | 鼠痘病毒 | ECT (Mousepox (Ectromelia) Virus) | 国标必检项 |
| 小鼠肝炎病毒 | MHV (Mouse hepatitis virus) | 国标必检项 | |
| 仙台病毒 | SV (Sendai virus) | 国标必检项 | |
| 小鼠肺炎病毒 | PVM (Pneumonia virus of Mice) | 国标必检项 | |
| 呼肠孤病毒Ⅲ型 | REO-3 (Reovirus type 3) | 国标必检项 | |
| 小鼠细小病毒（MVM株） | MVM (Minute virus of Mice) | 国标必检项 | |
| 汉坦病毒 | HV (Hantavirus) | 国标选做项 | |
| 淋巴细胞脉络丛脑膜炎病毒 | LCMV (Lymphocytic choriomeningitis virus) | 国标选做项 | |
| 小鼠脑脊髓炎病毒 | TMEV (Theiler’s mouse encephalomyelitis virus) | 国标选做项 | |
| 小鼠腺病毒 | MAV (Mouse adenovirus type 1(FL) & 2(K87)) | 国标选做项 | |
| 多瘤病毒 | Poly (Polyoma virus) | 国标选做项 | |
| 小鼠轮状病毒 | EDIM (Mouse rotavirus) | 国际标准选做项 | |
| 小鼠巨细胞瘤病毒 | MCMV (Mouse Cytomegalovirus) | 国际标准选做项 | |
| 乳酸脱氢酶增高病毒 | LDV （Lactate dehydrogenase-elevating virus） | 国际标准选做项 | |
| 小鼠细小病毒 | MPV (Mouse parvovirus) | 国际标准选做项 | |
| 小鼠细小病毒NS-1 | PARV-NS1 (Parvovirus NS-1) | 国际标准选做项 | |
| K病毒 | K virus | 国际标准选做项 | |
| 小鼠诺如病毒 | MNV (Murine norovirus) | 国际标准选做项 | |
| 病原菌 | 支原体 | MYCO (Mycoplasma) | 国标选做项 |
| 沙门菌 | SALM (Salmonella spp.) | 国标必检项 | |
| 鼠棒状杆菌 | CKUT (Corynebacterium kutscheri) | 国标必检项 | |
| 泰泽病原体 | CPIL (Clostridium piliforme (Tyzzer’s organism)) | 国标必检项 | |
| 噬肺巴斯德杆菌 | PPNE (Pasteurella pneumotropica) | 国标必检项 | |
| 金黄色葡萄球菌 | SAUR (Staphylococcus aureus) | 国标必检项 | |
| 绿脓杆菌 | PAER (Pseudomonsa aeruginosa) | 国标必检项 | |
| 肺炎克雷伯氏杆菌 | KPNE (Klebsiella pneumonia) | 国标必检项 | |
| 肺炎链球菌 | SPNE (Streptococcus pneumonia) | 国标选做项 | |
| 乙型溶血性链球菌 | BHS (Streptococcus (Beta-hemolytic)) | 国标选做项 | |
| 多杀巴斯德杆菌 | PMUL (Pasteurella multocida) | 国标选做项 | |
| 枸橼酸杆菌 | CROD (Citrobacter rodentium) | 国际标准选做项 | |
| 支气管鲍特杆菌 | BBRO (Bordetella bronchiseptica) | 国标选做项 | |
| 产酸克雷伯氏杆菌 | KOXY (Klebsiella oxytoca) | 国际标准选做项 | |
| 肺炎支原体 | MPUL (Mycoplasma pulmonis) | 国标必检项 | |
| 奇异变形杆菌 | PMIR (Proteus mirabilis) | 国际标准选做项 | |
| 乙型溶血性链球菌B群 | GBBHS (Beta strep.Sp.Group B) | 国标选做项 | |
| 乙型溶血性链球菌G群 | GGBHS (Beta strep.Sp.Group G) | 国标选做项 | |
| 寄生虫 | 体外寄生虫 | ECTO (Ectoparasites) | 国标必检项 |
| 弓形虫 | TOXO (Toxoplasma gondii) | 国标必检项 | |
| 全部蠕虫 | HELM (All Helminths) | 国标必检项 | |
| 鞭毛虫 | FLAG (Flagellates) | 国标必检项 | |
| 纤毛虫 | CILI (Ciliates) | 国标选做项 | |
| 兔脑原虫 | ECUN (Encephalitozoon cuniculi) | 国标选做项 |
Table 1
Supplementary Table 2
Microbiological quality control standards of MARC"
| 病原体 | 检测项目 | 英文名称 | 检测方法 |
|---|---|---|---|
| 病毒 | 鼠痘病毒 | ECT (Mousepox (Ectromelia) Virus) | 国标必检项 |
| 小鼠肝炎病毒 | MHV (Mouse hepatitis virus) | 国标必检项 | |
| 仙台病毒 | SV (Sendai virus) | 国标必检项 | |
| 小鼠肺炎病毒 | PVM (Pneumonia virus of Mice) | 国标必检项 | |
| 呼肠孤病毒Ⅲ型 | REO-3 (Reovirus type 3) | 国标必检项 | |
| 汉坦病毒 | HV (Hantavirus) | 国标选做项 | |
| 淋巴细胞脉络丛脑膜炎病毒 | LCMV (Lymphocytic choriomeningitis virus) | 国标选做项 | |
| 小鼠脑脊髓炎病毒 | TMEV (Theiler’s mouse encephalomyelitis virus) | 国标选做项 | |
| 小鼠腺病毒 | MAV (Mouse adenovirus type 1(FL) & 2(K87)) | 国标选做项 | |
| 多瘤病毒 | Poly (Polyoma virus) | 国标选做项 | |
| 小鼠轮状病毒 | EDIM (Mouse rotavirus) | 国际标准选做项 | |
| 小鼠巨细胞瘤病毒 | MCMV (Mouse Cytomegalovirus) | 国际标准选做项 | |
| 小鼠细小病毒 | MPV (Mouse parvovirus) | 国际标准选做项 | |
| K病毒 | K virus | 国际标准选做项 | |
| 病原菌 | 肺支原体 | MPUL (Mycoplasma pulmonis) | 国标必检项 |
| 沙门菌 | SALM (Salmonella spp.) | 国标必检项 | |
| 鼠棒状杆菌 | CKUT (Corynebacterium kutscheri) | 国标必检项 | |
| 泰泽病原体 | CPIL (Clostridium piliforme (Tyzzer’s organism)) | 国标必检项 | |
| 噬肺巴斯德杆菌 | PPNE (Pasteurella pneumotropica) | 国标必检项 | |
| 金黄色葡萄球菌 | SAUR (Staphylococcus aureus) | 国标必检项 | |
| 绿脓杆菌 | PAER (Pseudomonsa aeruginosa) | 国标必检项 | |
| 肺炎克雷伯氏杆菌 | KPNE (Klebsiella pneumonia) | 国标必检项 | |
| 肺炎链球菌 | SPNE (Streptococcus pneumonia) | 国标选做项 | |
| 乙型溶血性链球菌 | BHS (Streptococcus (Beta-hemolytic)) | 国标选做项 | |
| 皮肤病原真菌 | Pathogenic dermal fungi | 国标选做项 | |
| 假结核耶尔森菌 | Yersinia pseudotuberculosis | 国标选做项 | |
| 小肠结肠炎耶尔森菌 | Yesinia enterocolitica | 国标选做项 | |
| 念珠状链杆菌 | Streptobacillus moniliformis | 国标选做项 | |
| 大肠埃希菌O115 a,C,K(B) | Escherichia coli O115 a,C,K(B) | 国标选做项 | |
| 纤毛相关呼吸道杆菌 | CARB (Cilia-Associated Respiratory Bacillus) | 国际标准选做项 | |
| 寄生虫 | 体外寄生虫 | ECTO (Ectoparasites) | 国标必检项 |
| 弓形虫 | TOXO (Toxoplasma gondii) | 国标必检项 | |
| 蠕虫 | HELM (Helminth) | 国标必检项 | |
| 鞭毛虫 | FLAG (Flagellates) | 国标必检项 | |
| 卡式肺孢子虫 | Pneumocystis carinii | 国标选做项 | |
| 小鼠脑炎原虫 | ECUN (Encephalitozoon cuniculi) | 国标选做项 |
Supplementary Table 2
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