拟南芥ABI5基因对BR胁迫响应及其对下胚轴生长的调节作用

doi:10.16288/j.yczz.21-199

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Hereditas(Beijing) ›› 2021, Vol. 43 ›› Issue (9): 901-909.doi: 10.16288/j.yczz.21-199

• Research Article • Previous Articles Next Articles

Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana

Jianhui Lin¹(), Ziguang Liu², Nan Zhang¹, Rong Yan¹, Nan Zhang¹, Xinmiao He², Wentao Wang², Di Liu², Juan Wu¹()

1. College of Life Sciences, Northeast Forestry University, Key Laboratory of Northeast Salinity and Vegetation Restoration and Reconstruction, Ministry of Education, Harbin 150040, China
2. Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Key Laboratory of Combination of Planting and Breeding, Ministry of Agriculture and Rural Affairs, Harbin 150028, China

Received:2021-06-07 Revised:2021-08-16 Online:2021-09-20 Published:2021-09-09
Contact: Wu Juan E-mail:17862072380@139.com;wuj1970@163.com
Supported by:
Supported by the Special Fund for Basic Scientific Research Operation Fees of Central Universities No(2572020DY09);Heilongjiang Science Foundation Project No(LH2019C007);China Agriculture Research System of MOF and MARA

Abstract

Abstract:

Abscisic acid-insensitive 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor that is abundantly expressed in seeds. It plays a central role in regulating the abscisic acid (ABA) signal of seed germination and early seedling growth. Brassinosteroid (BR) is a new type of plant endogenous hormone, which has many physiological functions such as regulating plant growth and development and response to adversity stress. It has recently been discovered that under brassinolide stress, BIN2 (BRASSINOSTEROID INSENSITIVE2) and BES1 (BRI1-EMS-SUPPRESSOR 1) in the BR signaling pathway can inhibit the expression of ABI5 and promote Arabidopsis thaliana seed germination. In order to further explore the function of ABI5 under BR stress, this study analyzed the ABI5 expression characteristics during seed germination, identified Arabidopsis ABI5 gene deletion mutant abi5-1 and analyzed its function under BR stress, the results of which indicated that ABI5 was abundantly expressed in Arabidopsis dry seeds and responded to BR stress during germination. Under normal conditions, there was no significant difference between the hypocotyls of abi5-1 and wild-type seedlings; but under BR stress, the hypocotyls of abi5-1 seedlings were significantly longer than those of wild-type seedlings. These results reveal that ABI5 regulates the growth of Arabidopsis hypocotyls under BR stress, thereby providing a basis for in-depth understanding of the molecular mechanism of ABI5 regulation on plant development.

Key words: ABI5, brassinosteroid, Arabidopsis thaliana, hypocotyl

Jianhui Lin, Ziguang Liu, Nan Zhang, Rong Yan, Nan Zhang, Xinmiao He, Wentao Wang, Di Liu, Juan Wu. Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana[J]. Hereditas(Beijing), 2021, 43(9): 901-909.

Figures/Tables 6

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References 29

[1]	Finkelstein RR, Lynch TJ. The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor. Plant Cell, 2000, 12(4):599-609. pmid: 10760247
[2]	Skubacz A, Daszkowska-Golec A, Szarejko I. The role and regulation of ABI5 (ABA-Insensitive 5) in plant development, abiotic stress responses and phytohormone crosstalk. Front Plant Sci, 2016, 7:1884. doi: 10.3389/fpls.2016.01884 pmid: 28018412
[3]	Yu FF, Wu YR, Xie Q. Precise protein post-translational modifications modulate ABI5 activity. Trends Plant Sci, 2015, 20(9):569-575. doi: 10.1016/j.tplants.2015.05.004
[4]	Kim JH, Hyun WY, Nguyen HN, Jeong CY, Xiong LM, Hong SW, Lee H. AtMyb7, a subgroup 4 R2R3 Myb, negatively regulates ABA-induced inhibition of seed germination by blocking the expression of the bZIP transcription factor ABI5. Plant Cell Environ, 2015, 38(3):559-571. doi: 10.1111/pce.2015.38.issue-3
[5]	Wang TJ, Huang SZ, Zhang A, Guo P, Liu YT, Xu CM, Cong WX, Liu B, Xu ZY. JMJ17-WRKY40 and HY5- ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis. New Phytol, 2021, 230(2):567-584. doi: 10.1111/nph.v230.2
[6]	Bi C, Ma Y, Wang XF, Zhang DP. Overexpression of the transcription factor NF-YC9 confers abscisic acid hypersensitivity in Arabidopsis. Plant Mol Biol, 2017, 95(4-5):425-439. doi: 10.1007/s11103-017-0661-1
[7]	Yuan TT, Xu HH, Zhang KX, Guo TT, Lu YT. Glucose inhibits root meristem growth via ABA INSENSITIVE 5, which represses PIN1 accumulation and auxin activity in Arabidopsis. Plant Cell Environ, 2014, 37(6):1338-1350. doi: 10.1111/pce.2014.37.issue-6
[8]	Guan CM, Wang XC, Feng J, Hong SL, Liang Y, Ren B, Zuo JR. Cytokinin antagonizes abscisic acid-mediated inhibition of cotyledon greening by promoting the degradation of abscisic acid insensitive5 protein in Arabidopsis. Plant Physiol, 2014, 164(3):1515-1526. doi: 10.1104/pp.113.234740
[9]	Zhu JY, Sae-Seaw J, Wang ZY. Brassinosteroid signalling. Development, 2013, 140(8):1615-1620. doi: 10.1242/dev.060590
[10]	Nolan TM, Vukašinović N, Liu DR, Russinova E, Yin YH. Brassinosteroids: Multidimensional regulators of plant growth, development, and stress responses. Plant Cell, 2020, 32(2):295-318. doi: 10.1105/tpc.19.00335
[11]	Li ZY, Xu ZS, He GY, Yang GX, Chen M, Li LC, Ma YZ. A mutation in Arabidopsis BSK5 encoding a brassinosteroid- signaling kinase protein affects responses to salinity and abscisic acid. Biochem Biophys Res Commun, 2012, 426(4):522-527. doi: 10.1016/j.bbrc.2012.08.118
[12]	Ruan JX, Chen HH, Zhu T, Yu YG, Lei YW, Yuan LB, Liu J, Wang ZY, Kuang JF, Lu WJ, Huang SZ, Li CL. Brassinosteroids repress the seed maturation program during the seed-to-seedling transition. Plant Physiol, 2021, 186(1):534-548. doi: 10.1093/plphys/kiab089
[13]	Zhang DW, Tan WR, Yang F, Han Q, Deng XG, Guo HQ, Liu BH, Yin YH, Lin HH. A BIN2-GLK1 signaling module integrates brassinosteroid and light signaling to repress chloroplast development in the dark. Dev Cell, 2021, 56(3): 310-324.e7. doi: 10.1016/j.devcel.2020.12.001
[14]	Zhong CM, Patra B, Tang Y, Li XK, Yuan L, Wang XJ. A transcriptional hub integrating gibberellin-brassinosteroid signals to promote seed germination in Arabidopsis. J Exp Bot, 2021, 72(13):4708-4720. doi: 10.1093/jxb/erab192
[15]	Hu YR, Yu DQ. BRASSINOSTEROID INSENSITIVE2 interacts with ABSCISIC ACID INSENSITIVE5 to mediate the antagonism of brassinosteroids to abscisic acid during seed germination in Arabidopsis. Plant Cell, 2014, 26(11):4394-4408. doi: 10.1105/tpc.114.130849
[16]	Zhao X, Dou LR, Gong ZZ, Wang XF, Mao TL. BES1 hinders ABSCISIC ACID INSENSITIVE5 and promotes seed germination in Arabidopsis. New Phytol, 2019, 221(2):908-918. doi: 10.1111/nph.2019.221.issue-2
[17]	Guo HQ, Li L, Aluru M, Aluru S, Yin YH. Mechanisms and networks for brassinosteroid regulated gene expression. Curr Opin Plant Biol, 2013, 16(5):545-553. doi: 10.1016/j.pbi.2013.08.002
[18]	Wang HJ, Tang J, Liu J, Hu J, Liu JJ, Chen YX, Cai ZY, Wang XL. Abscisic acid signaling inhibits brassinosteroid signaling through dampening the dephosphorylation of BIN2 by ABI1 and ABI2. Mol Plant, 2018, 11(2):315-325. doi: 10.1016/j.molp.2017.12.013
[19]	Cary AJ, Liu W, Howell SH. Cytokinin action is coupled to ethylene in its effects on the inhibition of root and hypocotyl elongation in Arabidopsis thaliana seedlings. Plant Physiol, 1995, 107(4):1075-1082. pmid: 7770519
[20]	Stamm P, Topham AT, Mukhtar NK, Jackson MDB, Tomé DFA, Beynon JL, Bassel GW. The transcription factor ATHB5 affects GA-mediated plasticity in hypocotyl cell growth during seed germination. Plant Physiol, 2017: 173(1):907-917. doi: 10.1104/pp.16.01099
[21]	Wang HF, Shang QM. Molecular mechanisms of cell elongation in angiosperm hypocotyl. Bull Bot, 2018, 53(2):276-287.
	王红飞, 尚庆茂. 被子植物下胚轴细胞伸长的分子机理. 植物学报, 2018, 53(2):276-287.
[22]	Jiang N, Wang C, Pan JW. Molecular regulatory mechanisms of hypocotyl elongation and phototropism in Arabidopsis. J Plant Physiol, 2014, 50(10):1435-1444.
	姜楠, 王超, 潘建伟. 拟南芥下胚轴伸长与向光性的分子调控机理. 植物生理学报, 2014, 50(10):1435-1444.
[23]	Oh E, Zhu JY, Wang ZY. Interaction between BZR1 and PIF4 integrates brassinosteroid and environmental responses. Nat Cell Biol, 2012, 14(8):802-809. doi: 10.1038/ncb2545
[24]	Zhang YQ, Liu ZJ, Wang JF, Chen YD, Bi YR, He JX. Brassinosteroid is required for sugar promotion of hypocotyl elongation in Arabidopsis in darkness. Planta, 2015, 242(4):881-893. doi: 10.1007/s00425-015-2328-y
[25]	Zhong SW, Shi H, Xue C, Wei N, Guo HW, Deng XW. Ethylene-orchestrated circuitry coordinates a seedling's response to soil cover and etiolated growth. Proc Natl Acad Sci USA, 2014, 111(11):3913-3920. doi: 10.1073/pnas.1402491111
[26]	Kunihiro A, Yamashino T, Mizuno T. PHYTOCHROME- INTERACTING FACTORS PIF4 and PIF5 are implicated in the regulation of hypocotyl elongation in response to blue light in Arabidopsis thaliana. Biosci Biotechnol Biochem, 2010, 74(12):2538-2541. doi: 10.1271/bbb.100586
[27]	Li J, Terzaghi W, Gong YY, Li CR, Ling JJ, Fan YY, Qin NX, Gong XQ, Zhu DM, Deng XW. Modulation of BIN2 kinase activity by HY5 controls hypocotyl elongation in the light. Nat Commun, 2020, 11(1):1592. doi: 10.1038/s41467-020-15394-7
[28]	Li TT, Kang XK, Lei W, Yao XH, Zou LJ, Zhang DW, Lin HH. SHY2 as a node in the regulation of root meristem development by auxin, brassinosteroids, and cytokinin. J Integr Plant Biol, 2020, 62(10):1500-1517. doi: 10.1111/jipb.v62.10
[29]	Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H. Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis. Biotechnol Lett, 2008, 30(7):1281-1288. doi: 10.1007/s10529-008-9678-5 pmid: 18317696

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引物名称	引物序列(5ʹ→3ʹ)	复性温度(℃)	用途
Actin2-F	GGTAACATTGTGCTCAGTGGTGG	58	qRT-PCR
Actin2-R	AACGACCTTAATCTTCATGCTGC	58	qRT-PCR
abi5-1-F	CGAGGGTGGTGTTGGTGTCTTTA	60	突变体鉴定
abi5-1-R	CTACTCCATACTGACCTCCTA	60	突变体鉴定
ABI5-F(q)	AACCTAATCCAACCCGAACC	60	qRT-PCR
ABI5-R(q)	ACCCTCCTCCTCCTGTCC	60	qRT-PCR
BIN2-F(q)	ACAAAAGGATGCCCCCAGAA	59	qRT-PCR
BIN2-R(q)	TGAAGTTGAAGAGAGGCGGG	59	qRT-PCR
BES1-F(q)	GCAATTGTCTCCAAACACAGCAG	61	qRT-PCR
BES1-R(q)	CTCCAATCCTTCCTTCCGACATG	61	qRT-PCR
BZR1-F(q)	GCAGATGTCTCCAAATACTGCTG	61	qRT-PCR
BZR1-R(q)	GACATGCCATTTGGGTTTGCCTAG	61	qRT-PCR
PIN7-F(q)	GTGGGATGTGGCAATGCCTAA	60	qRT-PCR
PIN7-R(q)	TCCAATAGCCATTGCTGCCAC	60	qRT-PCR
EXP3-F(q)	GGAACTTGTACAGCCAAGGATA	57	qRT-PCR
EXP3-R(q)	AATAGATGGATTTCCCGGAACA	57	qRT-PCR

Stress response of ABI5 to BR stress and its regulation on hypocotyls growth in Arabidopsis thaliana

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