欧洲千里光花发育相关基因SvGLOBOSA功能研究

doi:10.16288/j.yczz.22-055

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Hereditas(Beijing) ›› 2022, Vol. 44 ›› Issue (6): 521-530.doi: 10.16288/j.yczz.22-055

• Research Article • Previous Articles Next Articles

Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris

Sinan Luan¹(), Lele Liu¹, Jiayuan Zhou¹, Nurasya Imam², Minlong Cui¹, Chunlan Piao¹()

1. Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China
2. Wushi County Bureau of Agriculture and Rural Affairs, Aksu 843000, China

Received:2022-03-22 Revised:2022-05-05 Online:2022-06-20 Published:2022-06-06
Contact: Piao Chunlan E-mail:1842239577@qq.com;2488335116@qq.com
Supported by:
Supported by Cooperative R & D project of Zhejiang Academy of Agricultural Sciences No(H20210384)

Abstract

Abstract:

The unique capitulum of Asteraceae has important ornamental and research value. Few studies have described the complex molecular mechanism of flower development. In this study, SvGLOBOSA(SvGLO), the MADS-box gene of Senecio vulgaris, was identified by screening the transcriptome data, and its function was examined. The gene structure was analyzed and its function was predicted through bioinformatics. The relative expression levels in different tissues of wild-type S. vulgaris were analyzed by qRT-PCR. SvGLO was overexpressed in Solanum nigrum and morphological observations were made. Histological staining was used in analyzing the histological changes in the ovary of transgenic S. nigrum. The results showed that the open reading frame of SvGLO was 591 bp long, encoding 196 amino acids. It has typical MADS-box and K-box domains and contains a PI motif at the C-terminal. SvGLO belongs to the PI/GLO subfamily of class B MADS-box genes. qRT-PCR results showed that SvGLO was highly expressed in inflorescence tissues but not in vegetative organs. In SvGLO-overexpressed S. nigrum, the sepals showed some characteristics of petals, carpels transformed into stamen-like organs, and fruit development was abnormal. Histological staining revealed that the morphology of ovary wall cells of transgenic S. nigrum was similar to that of anther wall cells of the stamen of wild-type S. vulgaris. Therefore, SvGLO may be involved in the regulation of petal and stamen development in S. vulgaris.

Key words: S. vulgaris, SvGLO, flower development, functional research

Sinan Luan, Lele Liu, Jiayuan Zhou, Nurasya Imam, Minlong Cui, Chunlan Piao. Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris[J]. Hereditas(Beijing), 2022, 44(6): 521-530.

Figures/Tables 8

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引物名称	引物序列(5′→3′)	用途
SvGLO	F: ATGGGTAGAGGAAAGATAGA	DNA鉴定
SvGLO	R: TCACATCCTCTCTTGCAAGTTA	DNA鉴定
NPT Ⅱ	F: AGATGGATTGCACGCAGGTTC	DNA鉴定
NPT Ⅱ	R: GAGGTCGAATGGGCAGGTAG	DNA鉴定
qSvGLO	F: CTTGAAAATGGGCTCACCAACA	qRT-PCR
qSvGLO	R: TCGTGACCTTGATAATCTCCCAAT	qRT-PCR
qSn18s	F: TTCTGTCGGCGATGCGTCC	qRT-PCR
qSn18s	R: GCCTCAAACTTCCGCGGCCT	qRT-PCR
qSv18s	F: ATAGCAGAACGACCTGTGAA	qRT-PCR
qSv18s	R: GAAGCAAGATCCAACGCAAT	qRT-PCR

Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris

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