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Hereditas(Beijing) ›› 2023, Vol. 45 ›› Issue (4): 273-278.doi: 10.16288/j.yczz.23-047

• Frontier Focus • Previous Articles     Next Articles

Research progress on the study of transcriptome-wide poly(A) tails in mammalian oocytes and early embryos

Wenlong Wang1,2(), Chunxia Zhang1,2()   

  1. 1. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Development Biology, Chinese Academy of Sciences, Beijing 100101, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2023-03-02 Revised:2023-03-13 Online:2023-04-20 Published:2023-03-14
  • Contact: Zhang Chunxia E-mail:wl.wang@genetics.ac.cn;cxzhang@genetics.ac.cn

Abstract:

During mammalian oocyte-to-embryo transition, before zygotic genome activation, the transcription in oocytes and embryos is silenced, so the post-transcriptional regulation of mRNA plays an essential role in this process. Poly(A) tail is an important post-transcriptional modification that affects mRNA metabolism and translation efficiency. With the development of sequencing technology and analysis tools, especially the methods based on third-generation sequencing technology, the length and composition of poly(A) tails can be accurately measured, greatly expanding our understanding of poly(A) tails in mammalian early embryonic development. This review focuses on the achievements of poly(A) tail sequencing methods and the research progress of poly(A) tail in regulating oocyte-to-embryo transition, discussing the future applications for the investigation of mammalian early embryonic development and infertility related diseases.

Key words: poly(A) tail, PAIso-seq, early embryogenesis, oocyte-to-embryo transition