植物RNA沉默抗病机制与应用研究进展

doi:10.16288/j.yczz.23-322

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Hereditas(Beijing) ›› 2024, Vol. 46 ›› Issue (4): 266-278.doi: 10.16288/j.yczz.23-322

• Invited Review • Next Articles

Advances in the mechanisms and applications of RNA silencing in crop protection

Wen Tian¹^,²(), Ting Chen¹^,²(), Qingyan Liu¹^,², Bosen Zhang¹, Huishan Guo¹^,²(), Jianhua Zhao¹^,²()

1. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
2. CAS Center for Excellence in Biotic Interactions, University of the Chinese Academy of Sciences, Beijing 100049, China

Received:2023-12-29 Revised:2024-03-13 Online:2024-04-20 Published:2024-03-22
Contact: Huishan Guo, Jianhua Zhao E-mail:tianwen9797@163.com;chent202202@163.com;guohs@im.ac.cn;zhaojh@im.ac.cn
Supported by:
Xinjiang Production and Construction Corps Science and Technology Plan Project(2022DB014)

Abstract

Abstract:

RNA silencing (or RNA interference, RNAi) is a conserved mechanism for regulating gene expression in eukaryotes, which plays vital roles in plant development and response to biotic and abiotic stresses. The discovery of trans-kingdom RNAi and interspecies RNAi provides a theoretical basis for exploiting RNAi-based crop protection strategies. Here, we summarize the canonical RNAi mechanisms in plants and review representative studies associated with plant-pathogen interactions. Meanwhile, we also elaborate upon the principles of host-induced gene silencing, spray-induced gene silencing and microbe-induced gene silencing, and discuss their applications in crop protection, thereby providing help to establish novel RNAi-based crop protection strategies.

Key words: RNA silencing, trans-kingdom RNAi, interspecies RNAi, host-induced gene silencing, spray-induced gene silencing, microbe-induced gene silencing

Wen Tian, Ting Chen, Qingyan Liu, Bosen Zhang, Huishan Guo, Jianhua Zhao. Advances in the mechanisms and applications of RNA silencing in crop protection[J]. Hereditas(Beijing), 2024, 46(4): 266-278.

Figures/Tables 3

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抗病技术	寄主植物	病原微生物	靶标基因	参考文献
HIGS	大麦	白粉病菌Blumeria graminis	Avra10	[65]
		禾谷镰刀菌Fusarium graminearum	Chs3b, CYP51A, CYP51B, CYP51C	[55, 56]
		黄色镰刀菌Fusarium culmorum	FcFgl1, FcGls1, FcFmk1, FcGls1	[57]
	小麦	条锈病菌Puccinia striiformis f. sp. tritici	PsFUZ7, PsCPK1	[66]
	棉花	大丽轮枝菌Verticillium dahliae	VdH1, VdILV2, VdILV6	[61, 63]
	油菜	核盘菌Sclerotinia sclerotiorum	PG, CBH, OAH1	[67]
	水稻	稻瘟病菌Magnaporthe oryzae	Mo ABC1, Mo MAC1, Mo PMK1, RHO1, MoAP1	[68, 69]
	玉米	拟轮枝镰孢菌Fusarium verticillioides	Deo, Atg15, Frp1	[70]
	玉米	黄曲霉Aspergillus flavus	Pc2, aflR, amy1	[71~73]
	马铃薯	疫霉Phytophthora infestans	PiGPB1	[60]
SIGS	大麦	禾谷镰刀菌Fusarium graminearum	DIC1, TRI15, CYP51A, CYP51B, CYP51C	[74, 75]
	大麦	核盘菌Sclerotinia sclerotiorum	β2Tub	[76]
	油菜	灰霉病菌Botrytis cinerea	Bctim44, Bctrr1	[77]
	油菜	核盘菌Sclerotinia sclerotiorum	SS1G_01703, SS1G_02495, SS1G_09897, SS1G_07873	[77]
	莴苣	灰霉病菌Botrytis cinerea	VPS51, SAC1, DCTN1, DCL1/DCL2	[70]
	番茄	黑曲霉Aspergillusniger	PS51, SAC1, DCTN1, FOW2, ChsV	[70, 78]
	水稻	稻瘟病菌Magnaporthe oryzae	AP1, SSADH, MAC1, PMK1	[79]
	拟南芥	禾谷镰刀菌 Fusarium graminearum	CYP51A, CYP51B, CYP5C	[80]

Advances in the mechanisms and applications of RNA silencing in crop protection

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[1]	XIE Zhao-Hui. The roles of RNA silencing in plant biotic stress [J]. HEREDITAS, 2010, 32(6): 561-570.
[2]	ZHU Jian, YU Chao, ZHU You-Lin. RNA silencing and its application in plant [J]. HEREDITAS, 2007, 29(1): 22-22―28.