Abstract:

Four hundred and five Qinchuan cattle at the age of 24 months were used to detect SNPs of adiponectin gene by PCR-SSCP and sequencing technology and to analyze the correlation of SNPs with carcass and meat quality traits using the general linear model (GLM) in SPSS program. Five genetypes (AA, AB, BB, CC, CD) were detected,with one G→C
mutation at 64 bp in exon2 of adiponectin in AB、BB genetypes and one C→T mutation at 50 bp in exon3 of adiponectin in
CD genetype. G→C mutation resulted glutamic acid (GGA) into glutamine (GCA) and C→T mutation resulted serine
(TCA) into leucine (TTA). Statistical analysis revealed that Qinchuan cattle with AA genotype was higher than BB genotype in slaughter weight, back fat thickness, carcass weight, loin muscle area (P<0.05). The crural girth of AA genotype was significantly higher than AB and BB genotypes (P<0.01). Qinchuan cattle with CD genotype was higher than CC genotype in slaughter weight, subcutaneous fat thickness, back fat thickness, crural girth, and tenderness (P<0.05). Adiponectin gene was proved to be closely related to carcass and meat quality traits (P<0.05), which can be used as a candidate molecular marker for production of high-grade meat in Qinchuan beef cattle.