二色补血草LbGRP基因的克隆及抗逆能力分析

doi:10.3724/SP.J.1005.2010.00278

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (3): 278-286.doi: 10.3724/SP.J.1005.2010.00278

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Cloning and stress tolerance analysis of an LbGRP gene in Limonium bicolor

PAN Yan, WANG Yu-Cheng, ZHANG Da-Wei, YANG Chuan-Ping

Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Ministry of Education, Harbin 150040, China

Received:2009-07-27 Revised:2009-09-26 Online:2010-03-20 Published:2010-03-15
Contact: YANG Chuan-Beng E-mail:yangchuanpingnefu@yahoo.com

Abstract

Abstract:

Glycine-rich RNA-binding proteins (GRP) are involved in post-transcriptional regulation, and play important roles in plant growth, development and stress response. In the present study, the full-length cDNA of a GRP gene (LbGRP, GenBank accession number: GQ398238) was cloned from a cDNA library of Limonium bicolor. To investigate the stress tolerance of LbGRP, the recombinant plasmid pYES2-LbGRP was constructed by inserting the LbGRP gene into the yeast expression vector pYES2. The recombinant plasmid pYES2-LbGRP was transformed into yeast Saccharomyces cerevisiae INVSc1, and the INVSc1 strain transformed with empty pYES2 was used as the control. The recombinant yeast INVSc1 harboring LbGRP (pYES2-LbGRP) and the control INVSc1 harboring empty pYES2 were treated with NaCl, KCl, NaHCO₃, Na₂CO₃, drought and frezzing, respectively, and their survial rates were compared under these stress conditions. The results showed that the survival rates of the recombinant yeast INVScl (pYES2-LbGRP) were higher than that of the control strain under these stress conditions, indicating that the LbGRP is tolerant to NaCl, KCl, NaHCO₃, Na₂CO₃, drought and frezzing stresses. These results suggested that LbGRP plays a role in stress tolerance of L. bicolor.

Key words: Limonium bicolor, glycine-rich RNA-binding protein, stress tolerance, yeast expression

BO Yan, WANG Yu-Cheng, ZHANG Da-Wei, YANG Chuan-Beng. Cloning and stress tolerance analysis of an LbGRP gene in Limonium bicolor[J]. HEREDITAS, 2010, 32(3): 278-286.

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Cloning and stress tolerance analysis of an LbGRP gene in Limonium bicolor

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