小麦近缘种低分子量麦谷蛋白亚基基因Glu-B3克隆及系统发育分析

doi:10.3724/SP.J.1005.2010.00613

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (6): 613-624.doi: 10.3724/SP.J.1005.2010.00613

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Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species

WANG Lin-Hai¹, ZHOU Min², LI Hui-Ling³, HE Zhong-Hu^{1, 4}, XIA Xian-Chun¹

1. Institute of Crop Science, National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Im-provement, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, China;
2. Beijing Agricultural College, Beijing 102206, China;
3. The Department of Seed Administration, Langfang 065000, China;
4. International Maize and Wheat Improvement Center (CIMMYT) China Office, Beijing 100081, China

Received:2009-10-15 Revised:2009-12-31 Online:2010-06-20 Published:2010-05-24
Contact: ZHOU Min;XIA Xian-Chun E-mail:zmskx@yahoo.com.cn;xiaxianchun@caas.net.cn

Abstract

Abstract:

The common wheat relative species are important germplasm for wheat breeding. In the present study, novel allelic variants at Glu-B3 locus were cloned to provide gene resources for wheat quality improvement. Four Glu-B3-locus specific primer sets LB1F/LB1R, LB2F/LB2R, LB3F/LB3R, and LB4F/LB4R were employed to isolate novel allelic variants of GluB3-1, GluB3-2, GluB3-3, and GluB3-4 from seven common wheat relative species, i.e., T. durum, T. dicoccum, T. dicoccoides, Aegilops longissima, Ae. searsii, Ae. Bicornis, and Ae. speltoides, and the software MEGA 4 was used to construct a phylogenetic tree. In total, 16 novel allelic variants of GluB3-1, GluB3-3, and GluB3-4 genes were isolated from the seven common wheat relative species, designated GluB3-16, GluB3-35, GluB3-36, GluB3-37, GluB3-46, GluB3-47, GluB3-48, GluB3-49, GluB3-410, GluB3-411, GluB3-412, GluB3-413, GluB3-414, GluB3-415, GluB3-416 and GluB3-417, respectively. In detail, GluB3-16 was cloned from T. dicoccoides with LB1F/LB1R, and the molecular weight of the de-duced amino acid was 39.2 kDa. GluB3-35, GluB3-36, and GluB3-37 were isolated from T. durum and T. dicoccum with the primer set LB3F/LB3R, and the molecular weights of their deduced peptides were 44.5 kDa (GluB3-36) and 44.6 kDa (GluB3-35 and GluB3-37). The molecular weight of deduced peptides of GluB3-4 ranged from 38.6 kDa (GluB3-414) to 42.5 kDa (GluB3-413). All the 16 new allelic variants showed a single open reading frame (ORF), and their deduced amino-acid sequences had a typical sequence structure of LMW-GS. The allelic variants at Glu-B3 locus identified in com-mon wheat relative species provide potential gene resources for wheat quality breeding and gene transformation. The results suggested that these Glu-B3 genes originated from different evolution processes.

Key words: Triticum aestivum L., wheat related species, LMW-GS, Glu-B3, phylogenetics

WANG Lin-Hai, ZHOU Min, LI Hui-Ling, HE Zhong-Hu, JIA Xian-Chun. Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species[J]. HEREDITAS, 2010, 32(6): 613-624.

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Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species

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