全基因组扩增技术及其在法医个体识别中的应用

doi:10.3724/SP.J.1005.2010.01119

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HEREDITAS ›› 2010, Vol. 32 ›› Issue (11): 1119-1125.doi: 10.3724/SP.J.1005.2010.01119

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Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification

CAI Hai-Qiang¹, LIU Hai-Tao², SHI Bin², LI An², TANG Wen-Ru¹, LUO Ying¹

1. Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China; 2. Criminal investigation team, Kunming public security bureau, Kunming 650224, China

Received:2010-01-23 Revised:2010-03-29 Online:2010-11-20 Published:2010-11-25
Contact: LUO Ying;TANG Wen-Ru E-mail:luoyingabc@yahoo.com;twr@sina.com

Abstract

Abstract: Whole genome amplification (WGA) is a technique that could non-selectively amplify the genomic DNA. In recent years, intensive studies have been made on application of WGA in trace DNA amplification, which might be applied to amplify trace DNA collected from crime scenes and provide enough DNA templates for forensic individual identification. However, the problem of amplification bias happened in the complicated test materials from real crime scenes is still troublesome. Development of WGA method with low amplification bias and high yield is the main target of current forensic research. Here, we reviewed the advances and the application prospect of WGA in individual identification. This might provide a reference for solving the problem of the amplification bias.

Key words: whole genome amplification (WGA), short tandem repeat, individual identification, forensic science

CA Hai-Jiang, LIU Hai-Chao, SHI Bin, LI An, TANG Wen-Ru, LUO Ying. Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification[J]. HEREDITAS, 2010, 32(11): 1119-1125.

References

[1] Jeffreys AJ, Wilson V, Thein SL. Individual-specific ‘fingerprints’ of human DNA. Nature, 1985, 316(6023): 76–79. [2] Leclair B, Sgueglia JB, Wojtowicz PC, Juston AC, Frégeau CJ, Fourney RM. STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes. J Forensic Sci, 2003, 48(5): 1001–1013. [3] Sun GY, Kaushal R, Pal P, Wolujewicz M, Smelser D, Cheng H, Lu M, Chakraborty R, Jin L, Deka R. Whole-genome amplification: relative efficiencies of the current methods. Leg Med (Tokyo), 2005, 7(5): 279–286. [4] Moore P. Photocopiers for DNA. Nature, 2005, 435(7039): 237. [5] Hosono S, Faruqi AF, Dean FB, Du YF, Sun ZY, Wu XH, Du J, Kingsmore SF, Egholm M, Lasken RS. Unbiased whole-genome amplification directly from clinical samples. Genome Res, 2003, 13(5): 954–964. [6] Hawkins TL, Detter JC, Richardson PM. Whole genome amplification-applications and advances. Curr Opin Biotechnol, 2002, 13(1): 65–67. [7] Hanson EK, Ballantyne J. Whole genome amplification strategy for forensic genetic analysis using single or few cell equivalents of genomic DNA. Anal Biochem, 2005, 346(2): 246–257. [8] Dean FB, Hosono S, Fang LH, Wu XH, Faruqi AF, Bray-Ward P, Sun ZY, Zong QL, Du YF, Du J, Driscoll M, Song W, Kingsmore SF, Egholm M, Lasken RS. Comprehensive human genome amplification using multiple displacement amplification. Proc Natl Acad Sci USA, 2002, 99(8): 5261–5266. [9] Cheung VG, Nelson SF. Whole genome amplification using a degenerate oligonucleotide primer allows hundreds of genotypes to be performed on less than one nanogram of genomic DNA. Proc Natl Acad Sci USA, 1996, 93(25): 14676–14679. [10] Kittler R, Stoneking M, Kayser M. A whole genome amplification method to generate long fragments from low quantities of genomic DNA. Anal Biochem, 2002, 300(2): 237–244. [11] Lee CIP, Leong SH, Png AEH, Choo KW, Syn C, Lim DTH, Law HY, Kon OL. An isothermal method for whole genome amplification of fresh and degraded DNA for comparative genomic hybridization, genotyping and mutation detection. DNA Res, 2006, 13(2): 77–88. [12] Daigo Y, Chin SF, Gorringe KL, Bobrow LG, Ponder BAJ, Pharoah PDP, Caldas C. Degenerate oligonucleotide primed-polymerase chain reaction-based array comparative genomic hybridization for extensive amplicon profiling of breast cancers : a new approach for the molecular analysis of paraffin-embedded cancer tissue. Am J Pathol, 2001, 158(5): 1623–1631. [13] Marchio A, Terris B, Meddeb M, Pineau P, Duverger A, Tiollais P, Bernheim A, Dejean A. Chromosomal abnormalities in liver cell dysplasia detected by comparative genomic hybridisation. Mol Pathol, 2001, 54(4): 270–274. [14] Lee YS, Tsai CN, Tsai CL, Chang SD, Hsueh DW, Liu CT, Ma CC, Lin SH, Wang TH, Wang HS. Comparison of whole genome amplification methods for further quantitative analysis with microarray-based comparative genomic hybridization. Taiwan J Obstet Gynecol, 2008, 47(1): 32–41. [15] Dietmaier W, Hartmann A, Wallinger S, Heinmöller E, Kerner T, Endl E, Jauch KW, Hofstädter F, Rüschoff J. Multiple mutation analyses in single tumor cells with improved whole genome amplification. Am J Pathol, 1999, 154(1): 83–95. [16] 陈玲, 王慧君, 刘超. 改良扩增前引物延伸技术检测痕量DNA. 医学研究生学报, 2007, 20(4): 442-442, 444. [17] Bergen AW, Haque KA, Qi Y, Beerman MB, Garcia-Closas M, Rothman N, Chanock SJ. Comparison of yield and genotyping performance of multiple displacement amplification and OmniPlex whole genome amplified DNA generated from multiple DNA sources. Hum Mutat, 2005, 26(3): 262–270. [18] Ballantyne KN, van Oorschot RAH, Mitchell RJ. Comparison of two whole genome amplification methods for STR genotyping of LCN and degraded DNA samples. Forensic Sci Int, 2007, 166(1

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Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification

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