Abstract: Based on the paired full-sib individuals selected from the established resource populations of Sutai pig that were characterized as resistant or sensitive to ETEC F18, Agilent double labeled cDNA microarray was used to identify the gene expression profiles in duodenum on purpose of investigating the genes related to Escherichia coli F18 receptor, which may cause edema disease and post-weaning diarrhea in piglets, as well as exploring the molecular mechanism about the differences involved in two different lineages. The results showed that thirteen differently expressed genes were found in one matched group including sensitive ones with GG genotype comparing with resistant ones with AA genotype at a two-fold filter, where there were 6 up-regulated genes and 7 down-regulated genes. In the other matched group composed of sensitive ones with AG genotype, 4 up-regulated genes and 2 down-regulated genes, 6 in total were screened out. GO analy-sis revealed that the differently expressed genes participated in many biological processes, such as immune response, ex-tracellular region, bacterial binding, response to external stimulus and so on. Meanwhile, these genes were mainly related to the Glycan Biosynthesis and Metabolism and Immune System pathways. Actually, the roles that they may play in edema disease and post-weaning diarrhea need further study and verification.

Key words: porcine, Escherichia coli F18, gene chip, gene expression profile