| [1] 卢宝荣, 张文驹, 李博. 转基因的逃逸及生态风险. 应用生态学报, 2003, 14(6): 989-994.[2] 章家恩, 骆世明. 农业生态安全及其生态管理对策探讨. 生态学杂志, 2004, 23(6): 59-62.[3] Daley M, Knauf VC, Summerfelt KR, Turner JC. Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep, 1998, 17(6-7): 489-496.[4] Komari T, Hiei Y, Saito Y, Murai N, Kumashiro T. Vectors carrying two separate T-DNAs for co-transformation for higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J, 1996, 10(1): 165-174.[5] Huang S, Gilbertson LA, Adams TH, Malloy KP, Reisen-bigler EK, Birr DH, Snyder MW, Zhang Q, Luethy MH. Generation of marker-free transgenic maize by regular two border Agrobacterium transformation vectors. Trans Res, 2004, 13(5): 451-461.[6] Ow DW. Recombinase-directed plant transformation for the post-genomic era. Plant Mol Biol, 2002, 48(1-2): 183-200.[7] Dale EC, Ow DW. Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA, 1991, 88(23): 10558-10562.[8] Lloyd AM, Davis RW. Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol Genet Genomics, 1994, 242(6): 653-657.[9] Shan XY, Li B, Zhang JR. Production of marker-free transgenic tobacco plants by FLP/frt recombination system. Chin J Biotechnol, 2006, 22(5): 744-749.[10] Fladung N, Nowitzki O, Kumar S, Hoenicka H. The site-specific recombination systems Cre-lox and FLP-FRT are functionally active in poplar. For Genet, 2005, 12(2): 121-130.[11] Fladung M, Becker D. Targeted integration and removal of transgenes in hybrid aspen (Populus tremula L. ×P. tremuloides Michx.) using site-specific recombination systems. Plant Biol (Stuttg), 2010, 12(2): 334-340.[12] Fladung M, Schenk TMH, Polak O, Becker D. Elimination of marker genes and targeted integration via FLP⁄FRT recombination system from yeast in hybrid aspen (Populus tremula L. ×P. tremuloides Michx.). Tree Genes Genomes, 2010, 6(2): 205-217.[13] Yoder JI, Goldsbrough AP. Transformation systems for generating marker-free transgenic plants. Nat Biotechnol, 1994, 12(3): 263-267.[14] Goldsbrough AP, Lastrella CN, Yoder JI. Transposition mediated re-positioningan subsequent eiimination of marker genes from transgenic tomato. Nat Biotechnol, 1993, 11(11): 1286-1292.[15] Zubko E, Seutt C, Meyer P. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol, 2000, 18(4): 442-445.[16] Fu XD, Duc LT, Fontana S, Bong BB, Tinjuangjun P, Sud-hakar D, Twyman RM, Christou P, Kohli A. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res, 2000, 9(1): 11-19.[17] 姚琴, 丛玲, 汪越胜, 陈明洁, 杨广笑, 何光源. 无载体框架序列转基因小麦中外源基因表达框的遗传分析. 遗传, 2006, 28(6): 695-698.[18] Romano A, Raemakers K, Bernardi J, Visser R, Mooibroek H. Transgene organisation in potato after particle bombardment-mediated (co-)transformation using plasmids and gene cassettes. Transgenic Res, 2003, 12(4): 461-473.[19] Gao XR, Wang GK, Su Q, Wang Y, An LJ. Phytase expression in transgenic soybeans: stable transformation with a vector-less construct. Biotechnol Lett, 2007, 29(11): 1781-1787.[20] Wu W, Su Q, Xia XY, Wang Y, Luan YS, An LJ. The Suaeda liaotungensis kitag betaine aldehyde dehy-drogenase gene improves salt tolerance of transgenic maize mediated with minimum linear length of DNA fragment. Euphytica, 2008, 159(1-2): 17-25.[21] Hohn B, |
