镜鲤体长性状的QTL定位分析

doi:10.3724/SP.J.1005.2011.01245

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (11): 1245-1250.doi: 10.3724/SP.J.1005.2011.01245

Mapping and genetic effect analysis of quantitative trait loci related to body length in mirror carp (Cyprinus carpio L.)

ZHANG Tian-Qi^1,2, ZHANG Xiao-Feng², TAN Zhao-Jun^1,2, CAO Zhu^1,2, WANG Xuan-Peng^2,3, LI Wen-Sheng^2,3, CHU Zhi-Yuan^1,2, SUN Xiao-Wen²

1. College of Life Science and Technology, Dalian Ocean University, Dalian 116023, China 2. Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China 3. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China

Received:2010-12-16 Revised:2011-03-30 Online:2011-11-20 Published:2011-11-25
Contact: SUN Xiao-Wen E-mail:sunxw2002@163.com

Abstract

Abstract: A group of F₂ hybrids of mirror carp including 68 individuals was used to construct a linkage map using JoinMap4.0 software. Of 560 markers genotyped, 507 markers (143 SSR markers, 33 EST-SSR markers, and 321 SNP markers) were assigned to the map, which comprised of 62 linkage groups. Quantitative traits loci (QTLs) associated with body length were identified by interval mapping of the software MapQTL 5.0. A linkage group wide permutation test (1 000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group. Twelve QTLs were identified for body length on the linkage groups of BL-1-1 (SNP0137-SNP1481), BL-4-1 (SNP0092-HLJ797), BL-5-1 (SNP1268-HLJ423), BL-7-1 (HLJ870-SNP0702), BL-12-1 (SNP0922-HLJ639), BL-16-1 (HLJE351-SNP0674), BL-25-1 (SNP0394-SNP0862), BL-35-1 (HLJ668-SNP0832), BL-43-1 (SNP0389-SNP1425), BL-47-1 (HLJ057-HLJ1113), BL-47-2 (HLJ1439-HLJ14180), which explained 13.8% to 64.9% of the total variation of body length. Eight major QTLs explained over 20% of the phenotypic variation, which were major QTLs associated with the body length of mirror carp.

Key words: mirror carp (Cyprinus carpio L.), body length, quantitative trait locus mapping, molecular maker–assisted selection

ZHANG Tian-Qi, ZHANG Xiao-Feng, TAN Zhao-Jun, CAO Zhu, WANG Xuan-Peng, LI Wen-Sheng, CHU Zhi-Yuan, SUN Xiao-Wen. Mapping and genetic effect analysis of quantitative trait loci related to body length in mirror carp (Cyprinus carpio L.)[J]. HEREDITAS, 2011, 33(11): 1245-1250.

References

[1] 孙效文, 鲁翠云, 匡友谊, 金万昆, 沈俊宝, 朱晓东, 李大宇, 马海涛, 于东梅. 镜鲤两个繁殖群体的遗传结构和几种性状的基因型分析. 水产学报, 2007, 31(3): 273-279.
[2] 梁利群, 孙效文. 鲤耐寒性状分子标记在遗传连锁图上的定位. 大连水产学院学报, 2003, 18(4): 278-281.
[3] Sun XW, Liang LQ. A genetic linkage map of common carp (Cyprinus earpio L.) and mapping of a locus associated with cold tolerance. Aquaculture, 2004, 238(1-4): 165-172.
[4] 张研, 梁利群, 常玉梅, 侯宁, 鲁翠云, 孙效文. 鲤鱼体长性状的QTL定位及其遗传效应分析. 遗传, 2007, 29(10): 1243-1248.
[5] 侯宁, 张研, 鲁翠云, 李勇, 李大宇, 季旭, 丁雷, 孙效文. 微卫星DNA标记分析德国镜鲤的遗传潜力. 遗传, 2007, 29(12): 1509-1518.
[6] 张义凤, 张研, 鲁翠云, 曹顶臣, 孙效文. 鲤鱼微卫星标记与体重、体长和体高性状的相关分析. 遗传, 2008, 30(5): 613-619.
[7] 刘继红, 张研, 常玉梅, 梁利群, 鲁翠云, 张晓峰, 徐美佳, 孙效文. 鲤鱼 (Cyprinus carpio L.) 头长、眼径、眼间距QTL的定位. 遗传, 2009, 31(5): 508-514.
[8] 伍献文. 中国鲤科鱼类志·上卷. 上海: 科学技术出版社, 1964: 4(绪论).
[9] Ohno S. The enormous diversity in genome sizes of fish as a reflection of natures′ extensive experiments with gene duplication. Trans Amer Fish Soc, 1970, 99: 120-130.
[10] 孙效文. 鱼类分子育种学. 北京: 海洋出版社, 2010, 5.
[11] 杨晶, 张晓峰, 储志远, 孙效文. 鲤的微卫星标记与体质量、体长、体高和吻长的相关分析. 中国水产科学, 2010, 17(4): 2-10.
[12] Tan YF, Xing YZ, Li JX, Yu SB, Xu CG, Zhang QF. Ge-netic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor Appl Genet, 2000, 101(5-6): 823-829.
[13] Xing YZ, Tan YF, Xu CG, Hua JP, Sun XL. Mapping quantitative trait loci for grain appearance traits of rice using a recombinant inbred line population. Acta Bot Sin, 2001, 43(8): 840-845.
[14] Reid DP, Szanto A, Glebe B, Danzmann RG, Ferguson MM. QTL for body weight and condition factor in Atlantic salmon (Salmo salar): comparative analysis with rainbow trout (Oncorhynchus mykiss) and Arctic charr (Salvelinus alpinus). Heredity, 2005, 94(2): 166-172.
[15] 岳志芹, 孔杰, 戴继勋. 水产动物遗传连锁图谱的研究现状及应用展望. 遗传, 2004, 26(1): 97-102.
[16] 孙效文, 张晓峰, 赵莹莹, 张研, 贾智英, 常玉梅, 鲁翠云, 梁利群. 水产生物微卫星标记技术研究进展及其应用. 中国水产科学, 2008, 15(4): 689-703.

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Mapping and genetic effect analysis of quantitative trait loci related to body length in mirror carp (Cyprinus carpio L.)

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