北京油鸡MSAP毛细管电泳荧光检测技术的建立

doi:10.3724/SP.J.1005.2014.0495

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HEREDITAS(Beijing) ›› 2014, Vol. 36 ›› Issue (5): 495-502.doi: 10.3724/SP.J.1005.2014.0495

Establishment of fluorescence labeling and capillary electrophoresis in MSAP for Beijing You chicken

Jinlong Li¹, Shaoqing Tang², Zhiyuan Zou³, Haichao Wang¹, Qing Xu¹

1. Institute of Life Science and Biotechnology, Beijing Jiaotong University, Beijing 100044, China;
2. Beijing Animal Husbandry Station, Beijing 100029, China;
3. Institute of Software, Beijing Jiaotong University, Beijing 100044, China

Received:2013-09-29 Revised:2013-12-09 Online:2014-05-20 Published:2014-05-25

Abstract

Abstract:

The DNA methylated states in muscle tissues from Beijing You chicken were analyzed using methylation sensitive amplified polymorphism (MSAP) combined with fluorescence labeling and capillary electrophoresis technology. We optimized the MSAP experimental condition including the genomic DNA concentration, dilution of pre-amplification prod-uct, the internal standard content and the concentrations of selective primers, Mg2+ and dNTPs. Repeated experiments showed that this method can automatically detect the global DNA methylation states in Beijing You chicken, and can be extended to other animals or plants with complex genomes and rich methylation polymorphism.

Key words: methylation sensitive amplified polymorphism (MSAP), capillary electrophoresis, fluorescence labeling, Beijing You chicken

Jinlong Li, Shaoqing Tang, Zhiyuan Zou, Haichao Wang, Qing Xu. Establishment of fluorescence labeling and capillary electrophoresis in MSAP for Beijing You chicken[J]. HEREDITAS(Beijing), 2014, 36(5): 495-502.

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Establishment of fluorescence labeling and capillary electrophoresis in MSAP for Beijing You chicken

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