Abstract: By using 265 pigs from eight breeds including Duroc,Landrace,Large White,Neijiang,Rongchang,Hanjiang Black,Hanzhong White,Bamei and wild ones, the genetic variations of 5`-upstream region from and the second intron in porcine H-FABP gene were checked by PCR-RFLP molecular marker with HinfI、Hae III and MspI，and effect of H-FABP gene on IMF content was then analyzed by least square analysis.The results showed as follows:(1) 8 pig breeds and wild pig had polymorphism at Hinf I-RFLP site. In above detected breeds,large white,Bamei pig, Hanjiang Black,Hanzhong White pig breeds and wild pig presented low polymorphism while other breeds have mediate polymorphism；(2)Among the tested breeds only 4 Chinese local pig breeds had no polymorphism at the Hae Ⅲ-RFLP and Msp I-RFLP sites,but Duroc,Landrace,Largewhite, Hanzhong White pig breeds and wild pig had polymorphism. Wild pig at the Hae Ⅲ-RFLP , Landrace,Largewhite and wild pig at the Hae Ⅲ-RFLP and Msp I-RFLP sites were low polymorphism,others were mediate polymorphism；（3）H-FABP gene increased IMF content significantly(P<0.05). Genetic effect of H-FABP gene on IMF content were HH>Hh>hh，DD