Abstract: The genetic variation in 5′- upstream （HinfⅠ-RFLP）and the second intron （HinfⅠ*-RFLP、Hae Ⅲ-RFLP）of heart fatty acid-binding protein（H-FABP）gene were detected with PCR-RFLP in 286 pigs including Mashen，Shanxi white pig and their crossbred populations. The results showed as follows：（1）Mashen presented only DD genotype while other populations varied，and Mashen crossbred populations had only 2 genotypes（DD、Dd）at the Hae Ⅲ-RFLP site；（2）At the HinfⅠ-RFLP site of the 5′-upstream region，the crossbred population of Shanxi white pig and Duroc presented only HH genotype while other populations varied. Frequency of h allele in Mashen was 0.9727. （3）At the HinfⅠ*-RFLP site of the second intron ，only Mashen presented 2 genotypes（BB、Bb），and frequency of B allele was 0.9667.（4）At the Hae Ⅲ-RFLP and HinfⅠ*-RFLP sites，all populations were in Hardy-weinberg equilibrium.