Abstract: The DNA sequence of a full-length Triticum astivum CV. Jinan 177ω-gliadin homologous gene (ω1236) containing partial 5′and 3′ flanking sequences with no intron was cloned by genomic PCR-based technology. Theω1236 sequence possibly encode a putative 47.2 kDa protein except for eight stop codons at amino acid residue positions 87, 117, 125, 157, 198, 313, 357 and 365 respectively. All the eight stop codons were caused by base transition. Sequence analysis revealed thatω1236 had 98% homology to aω-gliadin gene of wheat (AB059812). Like all other gliadin gene families characterized in cereals, this gene possessed all the features in other plant reported previously. Phylogenetic analysis of the completely sequenced gene as well as those ω-genes in wheat, ω-secalin and C-horden genes in rye and barley, and α-,β- andγ-gliadin genes in wheat indicated that theω1236 was more closely related toω-gliadin gene family, much less homology toα- , β- andγ-gliadin gene families. Short peptide was produced in the culture of transformed E. coli induced by IPTG in early 2 h. It indicated that stop codon would be inω1236. The result is consistent with that of the sequenced gene. The present paper could accumulate data useful for both ω-gliadin gene cloning by PCR and the study on structures and functions of these genes.