Abstract: The 5′ transcriptional promoter regulation region of FSHR gene was cloned and analyzed in Little-tailed Han sheep, Tan sheep and Australian Merino sheep. Comparison of 15 transcriptional regulatory elements in the FSHR gene showed no difference in the regulatory region of FSHR gene among the three breeds. The results showed no association between sheep breeds and 5′ transcriptional promoter regulation region and eliminated the possibility that the mutation of transcriptional regulation region can affect the transcription of FSHR gene in sheep.