Abstract: Peptide Nucleic Acids(PNA) are DNA analogues in which the naturally occurring nucleobases are attached via methylene carbonyl linkages to an achiral pseudopeptide backbone of N-(2-aminoethyl) glycine units. PNA can bind to both DNA and RNA targets in a sequence-specific manner. PNA provides a powerful tool to study the machanism of gene replication, transcription and an innovative strategy to regulate target gene expression. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilized for genetic investigations and diagnosis. Peptide Nucleic Acids(PNA) which recognizes and binds to complementary nucleic acid sequence presents unique physicochemical properties and has been incorporated into an expanding set of biological investigations.